LOCUS       sequence-389470-       10376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding human ErbB1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Matsuda K, Hirayama D, Hino N, Kuno S, Sakaue-Sawano A, Miyawaki A, 
            Matsuda M, Terai K
  TITLE     Knockout of all ErbB-family genes delineates their roles in 
            proliferation, survival, and migration.
  JOURNAL   J Cell Sci. 2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199.
  PUBMED    37519219
REFERENCE   2  (bases 1 to 10376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199."
COMMENT     Sequence Label: pPBbleo-ErbB1
FEATURES             Location/Qualifiers
     source          1..10376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1667
                     /label=chicken beta-actin promoter
     intron          1668..2684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2608..2630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2692..2711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    6494..7043
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6638..6655)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6865..6884
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7046..7420
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(7460..7477)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7466..7690
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7737..7753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7761..7777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7785..7791,7792..7809,7810..7815))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7830..7851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   8085..8147
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8188..8208)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8188..8206)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8227..8243)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8251..8267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8275..8281,8282..8299,8300..8305))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8320..8341
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8458..8475)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8629..9217)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8709..8728)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9388..10179,10180..10248))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10249..10353)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gsgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc
     1561 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     1621 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     1681 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     1741 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     1801 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     1861 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     1921 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     1981 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2041 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2101 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2161 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2221 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2281 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2341 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2401 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2461 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     2521 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     2581 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2641 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2701 tggttattgt gctgtctcat cattttggca aagaattggg atccgaattc ctcgagacca
     2761 tgcgaccctc cggggacggc cggggcagcg ctcctggcgc tgctggctgc gctctgcccg
     2821 gcgagtcggg ctctggagga aaagaaagtt tgccaaggca cgagtaacaa gctcacgcag
     2881 ttgggcactt ttgaagatca ttttctcagc ctccagagga tgttcaataa ctgtgaggtg
     2941 gtccttggga atttggaaat tacctatgtg cagaggaatt atgatctttc cttcttaaag
     3001 accatccagg aggtggctgg ttatgtcctc attgccctca acacagtgga gcgaattcct
     3061 ttggaaaacc tgcagatcat cagaggaaat atgtactacg aaaattccta tgccttagca
     3121 gtcttatcta actatgatgc aaataaaacc ggactgaagg agctgcccat gagaaattta
     3181 caggaaatcc tgcatggcgc cgtgcggttc agcaacaacc ctgccctgtg caacgtggag
     3241 agcatccagt ggcgggacat agtcagcagt gactttctca gcaacatgtc gatggacttc
     3301 cagaaccacc tgggcagctg ccaaaagtgt gatccaagct gtcccaatgg gagctgctgg
     3361 ggtgcaggag aggagaactg ccagaaactg accaaaatca tctgtgccca gcagtgctcc
     3421 gggcgctgcc gtggcaagtc ccccagtgac tgctgccaca accagtgtgc tgcaggctgc
     3481 acaggccccc gggagagcga ctgcctggtc tgccgcaaat tccgagacga agccacgtgc
     3541 aaggacacct gccccccact catgctctac aaccccacca cgtaccagat ggatgtgaac
     3601 cccgagggca aatacagctt tggtgccacc tgcgtgaaga agtgtccccg taattatgtg
     3661 gtgacagatc acggctcgtg cgtccgagcc tgtggggccg acagctatga gatggaggaa
     3721 gacggcgtcc gcaagtgtaa gaagtgcgaa gggccttgcc gcaaagtgtg taacggaata
     3781 ggtattggtg aatttaaaga ctcactctcc ataaatgcta cgaatattaa acacttcaaa
     3841 aactgcacct ccatcagtgg cgatctccac atcctgccgg tggcatttag gggtgactcc
     3901 ttcacacata ctcctcctct ggatccacag gaactggata ttctgaaaac cgtaaaggaa
     3961 atcacagggt ttttgctgat tcaggcttgg cctgaaaaca ggacggacct ccatgccttt
     4021 gagaacctag aaatcatacg cggcaggacc aagcaacatg gtcagttttc tcttgcagtc
     4081 gtcagcctga acataacatc cttgggatta cgctccctca aggagataag tgatggagat
     4141 gtgataattt caggaaacaa aaatttgtgc tatgcaaata caataaactg gaaaaaactg
     4201 tttgggacct ccggtcagaa aaccaaaatt ataagcaaca gaggtgaaaa cagctgcaag
     4261 gccacaggcc aggtctgcca tgccttgtgc tcccccgagg gctgctgggg cccggagccc
     4321 agggactgcg tctcttgccg gaatgtcagc cgaggcaggg aatgcgtgga caagtgcaac
     4381 cttctggagg gtgagccaag ggagtttgtg gagaactctg agtgcataca gtgccaccca
     4441 gagtgcctgc ctcaggccat gaacatcacc tgcacaggac ggggaccaga caactgtatc
     4501 cagtgtgccc actacattga cggcccccac tgcgtcaaga cctgcccggc aggagtcatg
     4561 ggagaaaaca acaccctggt ctggaagtac gcagacgccg gccatgtgtg ccacctgtgc
     4621 catccaaact gcacctacgg atgcactggg ccaggtcttg aaggctgtcc aacgaatggg
     4681 cctaagatcc cgtccatcgc cactgggatg gtgggggccc tcctcttgct gctggtggtg
     4741 gccctgggga tcggcctctt catgcgaagg cgccacatcg ttcggaagcg cacgctgcgg
     4801 aggctgctgc aggagaggga gcttgtggag cctcttacac ccagtggaga agctcccaac
     4861 caagctctct tgaggatctt gaaggaaact gaattcaaaa agatcaaagt gctgggctcc
     4921 ggtgcgttcg gcacggtgta taagggactc tggatcccag aaggtgagaa agttaaaatt
     4981 cccgtcgcta tcaaggaatt aagagaagca acatctccga aagccaacaa ggaaatcctc
     5041 gatgaagcct acgtgatggc cagcgtggac aacccacatg tctgtcggct gctgggcatc
     5101 tgcctcacct ccaccgtgca actcatcacg cagctcatgc ccttcggctg cctcctggac
     5161 tatgtccggg aacacaaaga caatattggc tcccagtacc tgctcaactg gtgtgtgcag
     5221 atcgcaaagg gcatgaacta cttggaggac cgtcgcttgg tgcaccgcga cctggcagcc
     5281 aggaacgtac tggtgaaaac accgcagcat gtcaagatca cagattttgg gctggccaaa
     5341 ctgctgggtg cggaagagaa agaataccat gcagaaggag gcaaagtgcc tatcaagtgg
     5401 atggcattgg aatcaatttt acacagaatc tatacccacc agagtgatgt ctggagctac
     5461 ggggtgaccg tttgggagtt gatgaccttt ggatccaagc catatgacgg aatccctgcc
     5521 agcgagatct cctccatcct ggagaaagga gaacgcctcc ctcagccacc catatgtacc
     5581 atcgatgtct acatgatcat ggtcaagtgc tggatgatag acgcagatag tcgcccaaag
     5641 ttccgtgagt tgatcatcga attctccaaa atggcccgag acccccagcg ctaccttgtc
     5701 attcaggggg atgaaagaat gcatttgcca agtcctacag actccaactt ctaccgtgcc
     5761 ctgatggatg aagaagacat ggacgacgtg gtggatgccg acgagtacct catcccacag
     5821 cagggcttct tcagcagccc ctccacgtca cggactcccc tcctgagctc tctgagtgca
     5881 accagcaaca attccaccgt ggcttgcatt gatagaaatg ggctgcaaag ctgtcccatc
     5941 aaggaagaca gcttcttgca gcgatacagc tcagacccca caggcgcctt gactgaggac
     6001 agcatagacg acaccttcct cccagtgcct gaatacataa accagtccgt tcccaaaagg
     6061 cccgctggct ctgtgcagaa tcctgtctat cacaatcagc ctctgaaccc cgcgcccagc
     6121 agagacccac actaccagga cccccacagc actgcagtgg gcaaccccga gtatctcaac
     6181 actgtccagc ccacctgtgt caacagcaca ttcgacagcc ctgcccactg ggcccagaaa
     6241 ggcagccacc aaattagcct ggacaaccct gactaccagc aggacttctt tcccaaggaa
     6301 gccaagccaa atggcatctt taagggctcc acagctgaaa atgcagaata cctaagggtc
     6361 gcgccacaaa gcagtgaatt tattggagca tgagcggccg ctctagagtc gacgggccgc
     6421 ggtaacaatt gttaactaac ttaagctagc aacggtttcc ctctagcggg atcaattccg
     6481 cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     6541 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     6601 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     6661 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     6721 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     6781 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     6841 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     6901 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     6961 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     7021 ttcctttgaa aaacacgata ataccatggc caagttgacc agtgccgttc cggtgctcac
     7081 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     7141 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     7201 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     7261 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     7321 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     7381 cggcaactgc gtgcacttcg tggccgagga gcaggactga aagtcgagat gcatcgatga
     7441 tctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     7501 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     7561 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     7621 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     7681 ggctctatgg cttctgaggc ggaaagaacc tgcagcccaa gcttggcgta atcatggtca
     7741 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7801 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7861 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatctt aaaagttttg
     7921 ttactttata gaagaaattt tgagtttttg ttttttttta ataaataaat aaacataaat
     7981 aaattgtttg ttgaatttat tattagtatg taagtgtaaa tataataaaa cttaatatct
     8041 attcaaatta ataaataaac ctcgatatac agaccgataa aacacatgcg tcaattttac
     8101 gcatgattat ctttaacgta cgtcacaata tgattatctt tctagggtta atctagtata
     8161 cgcgtttaat taatccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     8221 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8281 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8341 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8401 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8461 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8521 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8581 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8641 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8701 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8761 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8821 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8881 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8941 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9001 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9061 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9121 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9181 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9241 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9301 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9361 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9421 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9481 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9541 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9601 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9661 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9721 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9781 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9841 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9901 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9961 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10021 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10081 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10141 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10201 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10261 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10321 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
