LOCUS       sequence-389524-       16811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU6
            promtoer in M. oryzae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16811)
  AUTHORS   Zhang YM, Zheng L, Xie K
  TITLE     CRISPR/dCas9-Mediated Gene Silencing in Two Plant Fungal Pathogens.
  JOURNAL   mSphere. 2023 Feb 21;8(1):e0059422. doi: 10.1128/msphere.00594-22. 
            Epub 2023 Jan 19.
  PUBMED    36655998
REFERENCE   2  (bases 1 to 16811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere."; 
            date: "2023-02-21"; volume: "8(1):e0059422. doi"; pages: " 
            10.1128/msphere.00594-22. Epub 2023 Jan 19"
COMMENT     Sequence Label: pIF4
FEATURES             Location/Qualifiers
     source          1..16811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      163..730
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene"
     protein_bind    803..824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(839..844,845..862,863..869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    877..893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     882..904
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     901..917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     901..917
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1915..3042
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             3049..3102
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3103..4128
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3103..4128
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      4298..4865
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene"
     primer_bind     complement(4934..4953)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5192..5209)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5192..5208)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5201..5223)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5411..5435
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6736..7365
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7794..8867
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8933..9127
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9363..9385)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9471..9611
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9526..9545
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9626..9643)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9797..10385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9877..9896)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10472..11266)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11691..11715
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    12391..12415
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             12439..12465
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             12469..12489
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(12499..12522,12523..12525,12526..15081,15082..15084,
                     15085..16599)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(12499..12522,12523..12525,12526..15012,15013..15015,
                     15016..16599)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             16603..16623
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             16648..16770
                     /codon_start=1
                     /product="transcriptional repressor domain from the mouse 
                     Max-interacting protein 1 (Schreiber-Agus et al., 1995)"
                     /label=Mxi1
                     /translation="MERVRMINVQRLLEAAEFLERRERECEHGYASSFPSMPSPR"
     protein_bind    complement(join(16801..16811,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtacttaa cgttactgaa atcatcaaac agcttgacga atctggatat
       61 aagatcgttg gtgtcgatgt cagctccgga gttgagacaa atggtgttca ggatctcgat
      121 aagatacgtt catttgtcca agcagcaaag agtgccttct agtgatttaa tagctccatg
      181 tcaacaagaa taaaacgcgt ttcgggttta cctcttccag atacagctca tctgcaatgc
      241 attaatgcat tggacctcgc aaccctagta cgcccttcag gctccggcga agcagaagaa
      301 tagcttagca gagtctattt tcattttcgg gagacgagat caagcagatc aacggtcgtc
      361 aatggaccta cgagactgag gaatccgctc ttggctccac gcgactatat atttgtctct
      421 aattgtactt tgacatgctc ctcttcttta ctctgatagc ttgactatga aaattccgtc
      481 accagcccct gggttcgcaa agataattgc actgtttctt ccttgaactc tcaagcctac
      541 aggacacaca ttcatcgtag gtataaacct cgaaaatcat tcctactaag atgggtatac
      601 aatagtaacc atggttgcct agtgaatgct ccgtaacacc caatacgccg gccgaaactt
      661 ttttacaact ctcctatgag tcgtttaccc agaatgcaca ggtacacttg tttagaggta
      721 atccttcttt ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      781 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      841 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      901 caggaaacag ctatgacatg attacgaatt cgagctcggt acccggggat cctctagagt
      961 cgacctgcag gcatgcatag gctgaggctc acgccgttga gcctgcgcga taacaacaac
     1021 acgaacgaag gtcgggccat gtcggaatat gtgacgccgc cgatgatggc gggtgaaaga
     1081 gattctgcgg cttgaggggt cgcgacatgg gagttttcgt cgggtcatcc tttggtttac
     1141 agcagcaaac cattccactt tatgaagtgt attttgaagg ttcaagaggg cttcttaatc
     1201 tccggtcggg agagttatta tgacactcac tgcgaaagga tgccaaggta tctatttgtg
     1261 gcgatttccc aactcaccat acccttgggt tgctaatgtg cctggaaagg aagggtccag
     1321 ggatggattc gcaatcatcg gttgacagct caggatcagc aagaaagcca gtgctgccag
     1381 aggtctgtgc tgttggcaaa tgtgcagagg agagggacac ggccgtccaa ggtacataag
     1441 tcactttgtt ttggacttct ttcctaagga gaccgaggtc tcggttttga attgggtact
     1501 caaattggtt cccgcttgac gacattccga aacccccaat ttcgcggctt cgaatcgtgg
     1561 ctacccttgg ccgaggttgc gatttctctg ccgtatctga caatgatcca accggccgga
     1621 tgtgtgggag ggcgcaaagg gtgtaattgg gtctaggcgt gagctgcatg tccgatggca
     1681 gaaaaagaac tactaaaact ctgtgacgtc ctcagggctg gtagctattt agcttgcccc
     1741 tccctcaccc cacctagctc aaacccaaca aaacacagca ccgaccacaa aaatctgcct
     1801 cttaccacct gctcatcacc ttttctcaca tataaagcag tgagctcagc ccgatttcca
     1861 ttcctcaatt caagtctatt aactctctca aagaggaacc caatcttcag atccatggct
     1921 tcgtacccct gccatcaaca cgcgtctgcg ttcgaccagg ctgcgcgttc tcgcggccat
     1981 agcaaccgac gtacggcgtt gcgccctcgc cggcagcaag aagccacgga agtccgcctg
     2041 gagcagaaaa tgcccacgct actgcgggtt tatatagacg gtcctcacgg gatggggaaa
     2101 accaccacca cgcaactgct ggtggccctg ggttcgcgcg acgacatcgt ctacgtaccc
     2161 gagccgatga cttactggca ggtgctgggg gcttccgaga caatcgcgaa catctacacc
     2221 acacaacacc gcctcgacca gggtgagatt tcggccgggg acgcggcggt ggtaatgaca
     2281 agcgcccaga taacaatggg catgccttat gccgtgaccg acgccgttct ggctcctcat
     2341 atcggggggg aggctggcag ctcacatgcc ccgcccccgg ccctcaccct catcttcgac
     2401 cgccatccca tcgccgccct cctgtgctac ccggccgcgc gataccttat gggcagcatg
     2461 accccccagg ccgtgctggc gttcgtggcc ctcatcccgc cgaccttgcc cggcacaaac
     2521 atcgtgttgg gggcccttcc agaggacaga cacatcgacc gcctggccaa acgccagcgc
     2581 cccggcgagc ggcttgacct ggctatgctg gccgcgattc gccgcgttta cgggctgctt
     2641 gccaatacgg tgcggtatct ccagggcggc gggtcgtggc gggaggattg gggacagctt
     2701 tcggggacgg ccgtgccgcc ccagggtgcc gagccccaga gcaacgcggg cccacgaccc
     2761 catatcgggg acacgttatt taccctgttt cgggcccccg agttgctggc ccccaacggc
     2821 gacctgtaca acgtgtttgc ctgggccttg gacgtcttgg ccaaacgcct ccgtcccatg
     2881 cacgtcttta tcctggatta cgaccaatcg cccgccggct gccgggacgc cctgctgcaa
     2941 cttacctccg ggatggtcca gacccacgtc accacccccg gctccatacc gacgatctgc
     3001 gacctggcgc gcacgtttgc ccgcgagatg ggggaggcta accgtgccga gggtcgtggt
     3061 tcgctcctca cctgtggtga cgtcgaggag aaccctggtc ctatgaaaaa gcctgaactc
     3121 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     3181 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     3241 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     3301 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc
     3361 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     3421 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     3481 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     3541 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     3601 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     3661 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     3721 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     3781 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     3841 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
     3901 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     3961 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtagggcgt
     4021 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     4081 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagag tagatgccga
     4141 ccgggatcca cttaacgtta ctgaaatcat caaacagctt gacgaatctg gatataagat
     4201 cgttggtgtc gatgtcagct ccggagttga gacaaatggt gttcaggatc tcgataagat
     4261 acgttcattt gtccaagcag caaagagtgc cttctagtga tttaatagct ccatgtcaac
     4321 aagaataaaa cgcgtttcgg gtttacctct tccagataca gctcatctgc aatgcattaa
     4381 tgcattggac ctcgcaaccc tagtacgccc ttcaggctcc ggcgaagcag aagaatagct
     4441 tagcagagtc tattttcatt ttcgggagac gagatcaagc agatcaacgg tcgtcaagag
     4501 agttacgaga ctgaggaatc cgctcttggc tccacgcgac tatatatttg tctctaattg
     4561 tactttgaca tgctcctctt ctttactctg atagcttgac tatgaaaatt ccgtcaccag
     4621 cccctgggtt cgcaaagata attgcactgt ttcttccttg aactctcaag cctacaggac
     4681 acacattcat cgtaggtata aacctcgaaa atcattccta ctaagatggg tatacaatag
     4741 taaccatggt tgcctagtga atgctccgta acacccaata cgccggccga aactttttta
     4801 caactctcct atgagtcgtt tacccagaat gcacaggtac acttgtttag aggtaatcct
     4861 tctttctaga ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     4921 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcaccacttc
     4981 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     5041 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     5101 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agatctagag
     5161 gatcccccga ctagtgctga ggcattatgg cactggccgt cgttttacaa cgtcgtgact
     5221 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     5281 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     5341 gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac
     5401 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     5461 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
     5521 gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata
     5581 gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt
     5641 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt
     5701 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga
     5761 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca
     5821 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg
     5881 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg
     5941 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg
     6001 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca
     6061 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc
     6121 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga
     6181 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg
     6241 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc
     6301 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg
     6361 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc
     6421 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga
     6481 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc
     6541 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
     6601 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
     6661 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat
     6721 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
     6781 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
     6841 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
     6901 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
     6961 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
     7021 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
     7081 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
     7141 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
     7201 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
     7261 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
     7321 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
     7381 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
     7441 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
     7501 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
     7561 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg
     7621 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
     7681 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg
     7741 gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg
     7801 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     7861 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     7921 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     7981 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     8041 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     8101 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     8161 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     8221 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     8281 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     8341 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     8401 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     8461 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     8521 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     8581 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     8641 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     8701 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     8761 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     8821 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     8881 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa agcactcttt cctgtggata
     8941 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     9001 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     9061 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     9121 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     9181 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     9241 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     9301 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     9361 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     9421 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     9481 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     9541 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     9601 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9721 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9781 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9961 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10201 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    10261 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10321 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10441 gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat
    10501 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
    10561 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt
    10621 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga
    10681 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct
    10741 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc
    10801 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta
    10861 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg
    10921 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga
    10981 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga
    11041 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat
    11101 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc
    11161 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt
    11221 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc
    11281 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
    11341 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
    11401 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca
    11461 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
    11521 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
    11581 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
    11641 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
    11701 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta
    11761 atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt
    11821 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
    11881 tttcttatat gctcaacaca tgagcggaat tcataaatgt aggtattacc tgtacatttt
    11941 atttattcga gaaaaacaaa caaaccaaaa cgatgcagcg atcactaccg gcgtcatccg
    12001 gaattgaaac ggcgcgcgtt gaggggggcc ttcttgttcg gacacacacg gggccgccaa
    12061 agtggggccc atacggctgc ccatcccttg ctctccaata ccctggtcat gtgactcgaa
    12121 attggagact cacaaagatg ctttgaaacc ccgccgggaa ctttttctca cagacacaat
    12181 cagtcgacct tatcatcgca aaatcgacaa acctcaaaaa gaactcgaag agtcagtctc
    12241 ccttgcggtt aaactgattg atatctcgtg ttctctaaac cttcaataag tgagttactg
    12301 cgcacaaagc cgactatact gagcaccaac gcgaattgct cacgttgtcg cctaacagat
    12361 cttggctttc gtaggaaccc aatcttcaaa acaagtttgt acaaaaaagc aggctccgaa
    12421 ttcaccggtg ccaccatgta cccatacgat gttccagatt acgcttcgcc gaagaaaaag
    12481 cgcaaggtcg aagcgtccga caagaagtac agcatcggcc tggccatcgg caccaactct
    12541 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg
    12601 ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc
    12661 ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg
    12721 aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac
    12781 agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg
    12841 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc
    12901 taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat
    12961 ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac
    13021 cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg
    13081 ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga
    13141 ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat
    13201 ggcctgttcg gcaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac
    13261 ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg
    13321 gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac
    13381 ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc
    13441 cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg
    13501 aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc
    13561 aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc
    13621 atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga
    13681 gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac
    13741 ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac
    13801 aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg
    13861 gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc
    13921 tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg
    13981 accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac
    14041 gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga
    14101 aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc
    14161 aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc
    14221 gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac
    14281 gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt
    14341 ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg
    14401 ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga
    14461 tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc
    14521 ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag
    14581 ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc
    14641 cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag
    14701 ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag
    14761 cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag
    14821 aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc
    14881 ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac
    14941 ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac
    15001 tacgatgtgg acgccatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag
    15061 gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc
    15121 gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga
    15181 aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc
    15241 ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg
    15301 gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg
    15361 atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg
    15421 cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc
    15481 gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg
    15541 tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag
    15601 tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc
    15661 gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat
    15721 aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg
    15781 aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac
    15841 agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac
    15901 agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag
    15961 aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag
    16021 aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc
    16081 atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc
    16141 tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc
    16201 ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa
    16261 cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag
    16321 ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac
    16381 aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg
    16441 accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg
    16501 tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg
    16561 tacgagacac ggatcgacct gtctcagctg ggaggcgaca gccccaagaa gaagagaaag
    16621 gtggaggcca gcgggccggc cggatccatg gaacgtgtga gaatgattaa tgtgcaaagg
    16681 ctgttagaag ccgcagagtt tttagaaaga agagaaagag aatgcgaaca cgggtatgcc
    16741 agttctttcc ctagcatgcc ctctcccaga ggctaaatcg attgattaat taagaattcg
    16801 acccagcttt c
//
