LOCUS       sequence-389531-       10780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid for expressing the DNMT3bCD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10780)
  AUTHORS   Ma Y, Budde MW, Zhu J, Elowitz MB
  TITLE     Tuning Methylation-Dependent Silencing Dynamics by Synthetic 
            Modulation of CpG Density.
  JOURNAL   ACS Synth Biol. 2023 Aug 12. doi: 10.1021/acssynbio.3c00078.
  PUBMED    37572041
REFERENCE   2  (bases 1 to 10780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 12. doi: 10.1021/acssynbio.3c00078."
COMMENT     Sequence Label: vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD
FEATURES             Location/Qualifiers
     source          1..10780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(83..103)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        88..417
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      268..403
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     330..349
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        465..512
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             531..929
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(989..1009)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    1051..1172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   1935..1969
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2460..2476)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2460..2476)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2473..2495)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2484..2500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2508..2514,2515..2532,2533..2538))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2553..2574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2691..2708)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2862..3450)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2942..2961)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3621..4412,4413..4481))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4244..4263
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4482..4586)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4612..5067)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4749..4770)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4961..4980
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5194..5216
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5208..5225
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5209..5225
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   5355..5417
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     intron          6094..7032
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6989..7009
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        7065..7083
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             7144..7521
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             7528..8235
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7673..7691)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             8239..8292
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(10377..10396)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10378..10396)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    join(10670..10780,1..11)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aatgtatctt aaagacacct cgagaccatt accctgttat ccctagaatt cgcggccgca
       61 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
      121 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
      181 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      241 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
      301 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
      361 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
      421 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
      481 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagc
      541 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
      601 ccatctctga agattacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
      661 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
      721 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
      781 ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga
      841 tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
      901 tgccctctgg ttatgtgtgg gagggctaag tcgacaatca acctctggat tacaaaattt
      961 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     1021 ctttaatgcc tttgtatcat gcgttaacta aacttgttta ttgcagctta taatggttac
     1081 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     1141 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggaattgact caaatgatgt
     1201 caattagtct atcagaagct catctggtct cccttccggg ggacaagaca tccctgttta
     1261 atatttaaac agcagtgttc ccaaactggg ttcttatatc ccttgctctg gtcaaccagg
     1321 ttgcagggtt tcctgtcctc acaggaacga agtccctaaa gaaacagtgg cagccaggtt
     1381 tagccccgga attgactgga ttcctttttt agggcccatt ggtatggctt tttccccgta
     1441 tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc
     1501 gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg
     1561 agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac
     1621 gtaattacat ccctgggggc tttggggggg ggctgtccct gatatctata acaagaaaat
     1681 atatatataa taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga
     1741 gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt
     1801 atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc
     1861 ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca
     1921 atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt taatctagct
     1981 gcatcaggat catatcgtcg ggtctttttt ccggctcagt catcgcccaa gctggcgcta
     2041 tctgggcatc ggggaggaag aagcccgtgc cttttcccgc gaggttgaag cggcatggaa
     2101 agagtttgcc gaggatgact gctgctgcat tgacgttgag cgaaaacgca cgtttaccat
     2161 gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt gaactgttcg ttcaggccac
     2221 ctgggatacc agttcgtcgc ggcttttccg gacacagttc cggatggtca gcccgaagcg
     2281 catcagcaac ccgaacaata ccggcgacag ccggaactgc cgtgccggtg tgcagattaa
     2341 tgacagcggt gcggcgctgg gatattacgt cagcgaggac gggtatcctg gctggatgcc
     2401 gcagaaatgg acatggatac cccgtgagtt acccggcggg cgcgcttggc gtaatcatgg
     2461 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     2521 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     2581 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     2641 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     2701 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2761 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2821 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2881 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2941 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3001 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3061 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3121 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3181 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3241 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3301 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3361 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3421 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3481 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3541 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3601 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3661 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3721 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3781 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3841 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3901 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3961 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4021 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4081 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4141 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4201 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4261 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4321 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4381 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4441 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4501 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4561 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     4621 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     4681 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     4741 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     4801 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     4861 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     4921 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     4981 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     5041 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     5101 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     5161 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     5221 ccagtgagcg cgcctcgttc attcacgttt ttgaacccgt ggaggacggg cagactcgcg
     5281 gtgcaaatgt gttttacagc gtgatggagc agatgaagat gctcgacacg ctgcagaaca
     5341 cgcagctaga ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg
     5401 tgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa
     5461 tagatattaa gttttattat atttacactt acatactaat aataaattca acaaacaatt
     5521 tatttatgtt tatttattta ttaaaaaaaa caaaaactca aaatttcttc tataaagtaa
     5581 caaaactttt atgagggaca gccccccccc aaagccccca gggatgtaat tacgtccctc
     5641 ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc gctccccccg
     5701 catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg cacgggatcg
     5761 ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg ggatacgggg
     5821 aaaaggcctc cacggccaga attctaacta taacggtcct aaggtagcga aggtctcgcg
     5881 tactgtgagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5941 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     6001 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     6061 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     6121 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
     6181 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     6241 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     6301 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     6361 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     6421 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     6481 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     6541 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     6601 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     6661 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     6721 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     6781 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     6841 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     6901 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     6961 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     7021 cttccatttc aggtgtcgtg agctagcgat tacgccaagc tcgaaattaa ccctcactaa
     7081 agggaacaaa agctggagct ccaccgcggt ggcggccgct ctagcccggg cggatccgcc
     7141 accatgccag agccagcgaa gtctgctccc gccccgaaaa agggctccaa gaaggcggtg
     7201 actaaggcgc agaagaaagg cggcaagaag cgcaagcgca gccgcaagga gagctattcc
     7261 atctatgtgt acaaggttct gaagcaggtc caccctgaca ccggcatttc gtccaaggcc
     7321 atgggcatca tgaattcgtt tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc
     7381 cgcctggcgc attacaacaa gcgctcgacc atcacctcca gggagatcca gacggccgtg
     7441 cgcctgctgc tgcctgggga gttggccaag cacgccgtgt ccgagggtac taaggccatc
     7501 accaagtaca ccagcgctaa gcctaggatg gtgagcaagg gcgaggagga taacatggcc
     7561 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     7621 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     7681 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     7741 tacggctcca aggcctacgt gaagcacccc gccgacattc ccgactactt gaagctgtcc
     7801 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     7861 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     7921 accaacttcc cctccgacgg ccccgtaatg cagaagaaaa ccatgggctg ggaggcctcc
     7981 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     8041 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     8101 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     8161 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     8221 gacgagctgt acaagtctga gggcaggggg agcctcctaa cttgcggaga cgtcgaggag
     8281 aatcctggcc catctagact ggacaagagc aaagtcataa acggagctct ggaattactc
     8341 aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag
     8401 cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc
     8461 gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc atggcaagac
     8521 tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg cgacggggct
     8581 aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc
     8641 gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg
     8701 ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc aaaagaggaa
     8761 agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat tgagctgttc
     8821 gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg
     8881 gagaaacagc taaagtgcga aagcggcggg ggtagcggca gcggtagcgc atgcacaacc
     8941 aatgactctg ctgcttctga gtccccccca cccaagcgcc tcaagacaaa tagctatggc
     9001 gggaaggacc gaggggagga tgaggagagc cgagaacgga tggcttctga agtcaccaac
     9061 aacaagggca atctggagga ccgctgtttg tcctgtggaa agaagaaccc tgtgtccttc
     9121 caccccctct ttgagggtgg gctctgtcag agttgccggg atcgcttcct agagctcttc
     9181 tacatgtatg atgaggacgg ctatcagtcc tactgcaccg tgtgctgtga gggccgtgaa
     9241 ctgctgctgt gcagtaacac aagctgctgc agatgcttct gtgtggagtg tctggaggtg
     9301 ctggtgggcg caggcacagc tgaggatgcc aagctgcagg aaccctggag ctgctatatg
     9361 tgcctccctc agcgctgcca tggggtcctc cgacgcagga aagattggaa catgcgcctg
     9421 caagacttct tcactactga tcctgacctg gaagaatttc aggagccacc caagttgtac
     9481 ccagcaattc ctgcagccaa aaggaggccc attagagtcc tgtctctgtt tgatggaatt
     9541 gcaacggggt acttggtgct caaggagttg ggtattaaag tggaaaagta cattgcctcc
     9601 gaagtctgtg cagagtccat cgctgtggga actgttaagc atgaaggcca gatcaaatat
     9661 gtcaatgacg tccggaaaat caccaagaaa aatattgaag agtggggccc gttcgacttg
     9721 gtgattggtg gaagcccatg caatgatctc tctaacgtca atcctgcccg caaaggttta
     9781 tatgagggca caggaaggct cttcttcgag ttttaccact tgctgaatta tacccgcccc
     9841 aaggagggcg acaaccgtcc attcttctgg atgttcgaga atgttgtggc catgaaagtg
     9901 aatgacaaga aagacatctc aagattcctg gcatgtaacc cagtgatgat cgatgccatc
     9961 aaggtgtctg ctgctcacag ggcccggtac ttctggggta acctacccgg aatgaacagg
    10021 cccgtgatgg cttcaaagaa tgataagctc gagctgcagg actgcctgga gttcagtagg
    10081 acagcaaagt taaagaaagt gcagacaata accaccaagt cgaactccat cagacagggc
    10141 aaaaaccagc ttttccctgt agtcatgaat ggcaaggacg acgttttgtg gtgcactgag
    10201 ctcgaaagga tcttcggctt ccctgctcac tacacggacg tgtccaacat gggccgcggc
    10261 gcccgtcaga agctgctggg caggtcctgg agtgtaccgg tcatcagaca cctgtttgcc
    10321 cccttgaagg actactttgc ctgtgaatag ggtaccaggt aagtgtaccc aattcgccct
    10381 atagtgagtc gtattacaat tcactcgatc gcccttccca acagttgcgc agcctgaatg
    10441 gcgaatggag atccaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
    10501 tgtattttag attcacagtc ccaaggctca tttcaggccc ctcagtcctc acagtctgtt
    10561 catgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
    10621 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    10681 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    10741 tttttcactg cattctagtt gtggtttgtc caaactcatc
//
