LOCUS       sequence-38958-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Nesterov A, Carter RE, Sorkina T, Gill GN, Sorkin A
  TITLE     Inhibition of the receptor-binding function of clathrin adaptor 
            protein AP-2 by dominant-negative mutant mu2 subunit and its effects
            on endocytosis.
  JOURNAL   EMBO J. 1999 May 4;18(9):2489-99.
  PUBMED    10228163
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "1999-05-4"; volume: "18(9)"; pages: "2489-99"
COMMENT     Sequence Label: u2-HA-D176A
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      310..319
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctttgt tggtaaaatg atcggaggct tattcatcta taatcacaag
      121 ggggaggtgc ttatctcccg agtctacaga gatgacatcg ggaggaatgc agtggatgcc
      181 tttcgcgtca atgtcattca cgcacggcag caggtgcgca gccctgtcac aaacatcgct
      241 cgcaccagct tcttccatgt taagcggtcc aacatctggc tggcagcagt caccaagcag
      301 aatgtcaatg ccgccatggt cttcgaattc ctctacaaga tgtgtgatgt aatggctgct
      361 tactttggca agatcagcga ggagaacatc aagaacaatt tcgtgctcat atacgagctg
      421 ctggacgaga ttctggactt tggctaccca cagaactcag agacaggtgc actgaaaacc
      481 ttcatcaccc aacagggtat caagagtcag catcagacaa aagaagagca gtcccagatc
      541 accagccagg tgaccgggca aattggctgg aggcgggaag gcatcaagta tcgtcggaat
      601 gaactcttcc tagctgttct ggagagtgtg aacctgctta tgtccccaca ggggcaggtg
      661 ctgagtgccc atgtgtcggg ccgggtggtg atgaagagtt acctgagcgg ctg
//
