LOCUS       sequence-389645-        7743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacteria reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Schulte M, Olschewski K, Hensel M
  TITLE     The protected physiological state of intracellular Salmonella 
            enterica persisters reduces host cell-imposed stress.
  JOURNAL   Commun Biol. 2021 May 4;4(1):520. doi: 10.1038/s42003-021-02049-6.
  PUBMED    33947954
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-05-4"; volume: "4(1):520. doi"; pages: " 
            10.1038/s42003-021-02049-6"
COMMENT     Sequence Label: p5426
FEATURES             Location/Qualifiers
     source          1..7743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             341..352
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             356..1033
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASTEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(486..504)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(536..556)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     754..773
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     948..971
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             2405..2596
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2590..2612)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2698..2838
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2753..2772
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2853..2870)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3024..3612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3104..3123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3783..4574,4575..4643))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4406..4425
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4644..4735)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(4754..4781)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4873..4959)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5112..5129
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(5171..5884)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        5906..5961
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    5907..5925
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5937..5955
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             5977..6603
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVISSALELLNEVGIEGLTTRKLAQRLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(6053..6072)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             complement(6839..7717)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      301 ctagcaggag gaattcacca tggtcgatat cggtaccggg aaagaggaga aaagtatggc
      361 atccaccgag gacgtcatca aggagttcat gcgcttcaaa gtgcgcatgg agggctccgt
      421 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
      481 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
      541 cccccagttc cagtacggct ccaaggtgta cgtgaagcac cccgccgaca tccccgacta
      601 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
      661 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggctgcttca tctacaaggt
      721 gaagttcatc ggcgtgaact tcccctccga cggccccgta atgcagaaga agactatggg
      781 ctgggagccc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
      841 caaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaagt ccatctacat
      901 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gactccaagc tggacatcac
      961 ctcccacaac gaggactaca ccatcgtgga gcagtacgaa cgcaccgagg gccgccacca
     1021 cctgttcctg tagccatgga tcgatagctg gtcgacaacc attccttgcg gcggcggtgc
     1081 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
     1141 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     1201 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     1261 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
     1321 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
     1381 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
     1441 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
     1501 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
     1561 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
     1621 tcattggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
     1681 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
     1741 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
     1801 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
     1861 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg
     1921 cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct
     1981 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg
     2041 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
     2101 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat
     2161 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg
     2221 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt
     2281 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat
     2341 cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc
     2401 atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac
     2461 attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga
     2521 atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga
     2581 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     2641 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     2701 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     2761 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     2821 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2881 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2941 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3001 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3061 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3121 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3181 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3241 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3301 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3361 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3421 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     3481 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3541 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3601 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3661 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3721 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3781 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3841 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3901 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3961 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4021 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4081 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4141 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4201 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4261 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4321 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4381 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     4441 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4501 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4561 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4621 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4681 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaaagagt
     4741 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttaattt gatgcctggc
     4801 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcgcaa cgttcaaatc
     4861 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     4921 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     4981 tcgcatgggg agaccccaca ctaccatcgg cgctacggcg tttcacttct gagttcggca
     5041 tggggtcagg tgggaccacc gcgctactgc cgccaggcaa attctgtttt atcagaccgc
     5101 ttctgcgttc tgatttaatc tgtatcaggc tgaaaatctt ctctcatccg ccaaaacaga
     5161 agttttatta tttatacagt tcatccatgc catgcgtaat acccgccgcg gtcacaaatt
     5221 ccagcagcac catatggtcg cgtttttcgt tcggatcttt gctcaggacg ctctgggtgc
     5281 tcagataatg gttatccggc agcagcaccg ggccatcgcc aatcggggtg ttctgctgat
     5341 aatgatccgc cagctgcacg ctgccatctt ccacgttgtg gcggatttta aagttcgctt
     5401 tgatgccgtt tttctgttta tccgcggtaa tatacacgtt atggctgtta aagttgtatt
     5461 ccagtttatg gcccaggatg ttgccatctt ctttaaaatc gatgcctttc agttcaatgc
     5521 ggttcaccag ggtatcgcct tcaaatttca cttccgcacg ggttttatag gtgccatcat
     5581 ctttaaagct aatggtgcgt tcctgcacat agccttccgg catcgcgctt ttaaaaaaat
     5641 catgctgttt catatgatcc ggataacgcg caaagcactg cacgccatag gtcagggtgg
     5701 tcaccagggt cggccacggc accggcagtt tgcccgtggt gcaaatgaat ttcagggtca
     5761 gtttgccgtt ggtcgcatcg ccttcgcctt cgccacgcac gctaaattta tggccgttca
     5821 catcgccatc cagttccacc agaatcggca ccacaccggt aaacagttct tcgcctttgc
     5881 gcatgatatc ctcctcttgc catctttctc tatcactgat agggagtggt aaaataactc
     5941 tatcaatgat agagtgtcaa caaaaattag gaattaatga tgtctagatt agataaaagt
     6001 aaagtgatta gcagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc
     6061 cgtaaactcg cccagaggct aggtgtagag cagcctacat tgtattggca tgtaaaaaat
     6121 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt
     6181 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga
     6241 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa
     6301 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta
     6361 gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa
     6421 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg
     6481 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta
     6541 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtct
     6601 taaaagcagc ataacctttt tccgtgatgg taacttcacg gtaaccaaga tgtcgagtta
     6661 accacccatc gaagccaccg cggtggagag aaaataataa aaaagccgga ttaataatct
     6721 ggctttttat attctctgcg gccatcgatg cataatgtgc ctgtcaaatg gacgaagcag
     6781 ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt
     6841 atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg
     6901 ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa
     6961 cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc
     7021 tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat
     7081 gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc
     7141 tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg
     7201 gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta
     7261 tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca
     7321 ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata
     7381 ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt
     7441 gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca
     7501 gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac
     7561 cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat
     7621 cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt
     7681 atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca
     7741 gag
//
