LOCUS       sequence-389649-        7634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacteria reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Schulte M, Olschewski K, Hensel M
  TITLE     The protected physiological state of intracellular Salmonella 
            enterica persisters reduces host cell-imposed stress.
  JOURNAL   Commun Biol. 2021 May 4;4(1):520. doi: 10.1038/s42003-021-02049-6.
  PUBMED    33947954
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-05-4"; volume: "4(1):520. doi"; pages: " 
            10.1038/s42003-021-02049-6"
COMMENT     Sequence Label: p4928
FEATURES             Location/Qualifiers
     source          1..7634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..48
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     RBS             73..84
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      874..901
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(931..950)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(932..950)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(960..977)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(960..976)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(969..991)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1118..1573
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1205..1224)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1415..1436
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1599..1703
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1704..1772,1773..2564)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1922..1941)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(3585..4535)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4583..4805
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     complement(5947..5967)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             complement(6042..6755)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        6777..6832
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    6778..6796
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6808..6826
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             6848..7474
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVISSALELLNEVGIEGLTTRKLAQRLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(6924..6943)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgactc agggcccgat
       61 atcggtaccg ggaaagagga gaaaagtatg gtgagcaaag gcgaagaact gattaaagaa
      121 aacatgcata tgaaactgta tatggaaggc accgtgaaca accatcattt taaatgcacc
      181 agcgaaggcg aaggcaaacc gtatgaaggc acccagacca tgcgcattaa agtggtggaa
      241 ggcggtccgc tgccgtttgc gtttgatatt ctggcgacca gctttatgta tggcagccgc
      301 acctttatta accataccca gggcattccg gattttttta aacagagctt tccggaaggc
      361 tttacctggg aacgcgtgac cacctatgaa gatggcggcg tgctgaccgc gacccaggat
      421 accagcctgc aggatggctg cctgatttat aacgtgaaaa ttcgtggcgt gaactttccg
      481 agcaatggcc cggtgatgca gaaaaaaacc ctgggctggg aagcgaacac cgaaatgctg
      541 tatccggcgg atggcggcct ggaaggccgt accgatatgg cgctgaaact ggtgggcggt
      601 ggccatctga tttgcaactt taaaaccacc tatcgcagca aaaaaccggc gaaaaacctg
      661 aaaatgccgg gcgtgtatta tgtggatcat cgtctggaac gcattaaaga agcggataaa
      721 gaaacctatg tggaacagca tgaagtggcg gtggcgcgtt attgcgatct gccgagcaaa
      781 ctgggccata aactgaacgg catggatgaa ctgtataaat aataaccatg gatcgatagc
      841 tggtgttaac gaattccagg catcaaatta agcagaaggc catcctgacg gatggccttt
      901 ttgcgtttct acaaactgag ctccaattcg ccctatagtg agtcgtatta cgcgcgctca
      961 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     1021 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     1081 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     1141 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     1201 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     1261 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     1321 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     1381 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     1441 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     1501 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     1561 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1621 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1681 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     1741 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     1801 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     1861 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     1921 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     1981 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2041 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2101 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2161 tgggggatca tgtaactcgc cttgatcgct gggaaccgga gctgaatgaa gccataccaa
     2221 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2281 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2341 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2401 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2461 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     2521 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     2581 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2641 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2701 cgtcagatct ctgttcgggg gttcactgac gctgcgtgac tacgatggtc aggttgcgga
     2761 ggctatggcc ctggtacgag cgctgaacaa aatgacgaaa gcaggtatgc ctgaaagcgt
     2821 gcgtattgcc tgaaaacaca acccgctacg ggggagactt acccgaaatc tgatttattc
     2881 aacaaagcca ctttcagtat ggatttgggt gttacggggg ggggttggtg cttaaaatcg
     2941 cgctgaggat ttctgatggt gttaagcggg cggttttgag atgtaaactc gcccgtttaa
     3001 cataatggat cttgcgcgca ccgcccgaac accactcgcc acaaaaaacc gccggaacgt
     3061 ccaaaagtac gggttttgct gcccgcaaac gggctgttct ggtgttgcta gtttgttatc
     3121 agaatcgcag atccggcttc agccggtttg ccggctgaaa gcgctatttc ttccagaatt
     3181 gccatgattt tttccccacg ggaggcgtca ctggctcccg tgttgtcggc agctttgatt
     3241 cgataagcag catcgcctgt ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt
     3301 gtctcaggtg ttcaatttca tgttctagtt gctttgtttt actggtttca cctgttctat
     3361 taggtgttac atgctgttca tctgttacat tgtcgatctg ttcatggtga acagctttga
     3421 atgcaccaaa aactcgtaaa agctctgatg tatctatctt ttttacaccg ttttcatctg
     3481 tgcatatgga cagttttccc tttgatatgt aacggtgaac agttgttcta cttttgtttg
     3541 ttagtcttga tgcttcactg atagatacaa gagccataag aacctcagat ccttccgtat
     3601 ttagccagta tgttctctag tgtggttcgt tgtttttgcg tgagccatga gaacgaacca
     3661 ttgagatcat acttactttg catgtcactc aaaaattttg cctcaaaact ggtgagctga
     3721 atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc cgttatgtag gtaggaatct
     3781 gatgtaatgg ttgttggtat tttgtcacca ttcattttta tctggttgtt ctcaagttcg
     3841 gttacgagat ccatttgtct atctagttca acttggaaaa tcaacgtatc agtcgggcgg
     3901 cctcgcttat caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt acttattggt
     3961 ttcaaaaccc attggttaag ccttttaaac tcatggtagt tattttcaag cattaacatg
     4021 aacttaaatt catcaaggct aatctctata tttgccttgt gagttttctt ttgtgttagt
     4081 tcttttaata accactcata aatcctcata gagtatttgt tttcaaaaga cttaacatgt
     4141 tccagattat attttatgaa tttttttaac tggaaaagat aaggcaatat ctcttcacta
     4201 aaaactaatt ctaatttttc gcttgagaac ttggcatagt ttgtccactg gaaaatctca
     4261 aagcctttaa ccaaaggatt cctgatttcc acagttctcg tcatcagctc tctggttgct
     4321 ttagctaata caccataagc attttcccta ctgatgttca tcatctgagc gtattggtta
     4381 taagtgaacg ataccgtccg ttctttcctt gtagggtttt caatcgtggg gttgagtagt
     4441 gccacacagc ataaaattag cttggtttca tgctccgtta agtcatagcg actaatcgct
     4501 agttcatttg ctttgaaaac aactaattca gacatacatc tcaattggtc taggtgattt
     4561 taatcactat accaattgag atgggctagt caatgataat tactagtcct tttcctttga
     4621 gttgtgggta tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa ttctgctaga
     4681 ccctctgtaa attccgctag acctttgtgt gttttttttg tttatattca agtggttata
     4741 atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat
     4801 aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct
     4861 cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc
     4921 aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct gtctttttcg
     4981 tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat ggcactacag
     5041 gcgcctttta tggattcatg caaggaaact acccataata caagaaaagc ccgtcacggg
     5101 cttctcaggg cgttttatgg cgggtctgcg gagtggtgaa tccgttagcg aggtgccgcc
     5161 ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca
     5221 gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc
     5281 ggcataaatc gccgtgacga tcagcggtcc aatgatcgaa gttaggctgg taagagccgc
     5341 gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg
     5401 caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca
     5461 gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat
     5521 aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag
     5581 ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa
     5641 gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc ctgtcctacg agttgcatga
     5701 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
     5761 tgactgggtt gaaggctctc aagggcatcg gcattcgcca ttcaggctgc ctcctaccaa
     5821 gacgacttca aatccgaccc gtagagatcg taccgggccc cccctcgaga aaaaaaagcc
     5881 cgctcattag gcgggctagc tcattatttg tagagctcat ccatgccatg tgtaatccca
     5941 gcagcagtta caaactcaag aaggaccatg tggtcacgct tttcgttggg atctttcgaa
     6001 agggcagatt gtgtcgacca gctatcgatc catggttatt atttatacag ttcatccatg
     6061 ccatgcgtaa tacccgccgc ggtcacaaat tccagcagca ccatatggtc gcgtttttcg
     6121 ttcggatctt tgctcaggac gctctgggtg ctcagataat ggttatccgg cagcagcacc
     6181 gggccatcgc caatcggggt gttctgctga taatgatccg ccagctgcac gctgccatct
     6241 tccacgttgt ggcggatttt aaagttcgct ttgatgccgt ttttctgttt atccgcggta
     6301 atatacacgt tatggctgtt aaagttgtat tccagtttat ggcccaggat gttgccatct
     6361 tctttaaaat cgatgccttt cagttcaatg cggttcacca gggtatcgcc ttcaaatttc
     6421 acttccgcac gggttttata ggtgccatca tctttaaagc taatggtgcg ttcctgcaca
     6481 tagccttccg gcatcgcgct tttaaaaaaa tcatgctgtt tcatatgatc cggataacgc
     6541 gcaaagcact gcacgccata ggtcagggtg gtcaccaggg tcggccacgg caccggcagt
     6601 ttgcccgtgg tgcaaatgaa tttcagggtc agtttgccgt tggtcgcatc gccttcgcct
     6661 tcgccacgca cgctaaattt atggccgttc acatcgccat ccagttccac cagaatcggc
     6721 accacaccgg taaacagttc ttcgcctttg cgcatgatat cctcctcttg ccatctttct
     6781 ctatcactga tagggagtgg taaaataact ctatcaatga tagagtgtca acaaaaatta
     6841 ggaattaatg atgtctagat tagataaaag taaagtgatt agcagcgcat tagagctgct
     6901 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaggc taggtgtaga
     6961 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat
     7021 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga
     7081 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc
     7141 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt
     7201 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt
     7261 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga
     7321 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt
     7381 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt
     7441 agaaaaacaa cttaaatgtg aaagtgggtc ttaaaagcag cataaccttt ttccgtgatg
     7501 gtaacttcac ggtaaccaag atgtcgagtt aaccacccat cgaagccacc gcggtggaga
     7561 gaaaataata aaaaagccgg attaataatc tggcttttta tattctctgc ggccgcgttt
     7621 aaacccatgg acgt
//
