LOCUS       sequence-389652-       13520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of hCOL4A4_R1683Xin mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13520)
  AUTHORS   Omachi K, Kai H, Roberge M, Miner JH
  TITLE     NanoLuc reporters identify COL4A5 nonsense mutations susceptible to 
            drug-induced stop codon readthrough.
  JOURNAL   iScience. 2022 Feb 8;25(3):103891. doi: 10.1016/j.isci.2022.103891. 
            eCollection 2022 Mar 18.
  PUBMED    35243249
REFERENCE   2  (bases 1 to 13520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-02-8"; volume: "25(3):103891. doi"; pages: " 
            10.1016/j.isci.2022.103891. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pLV-Puro_CI-hCOL4A5-Nluc_R1683X
FEATURES             Location/Qualifiers
     source          1..13520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             13..646
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    693..818
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1315..1548
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1733..1777
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2040..2155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2223..2602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2603..2806
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2757..2777
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          2942..3074
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3057..3076
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     3118..3137
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3118..3136
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8240..8752
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     promoter        8768..9267
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8803..8826)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9200..9219
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9287..9886
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9287..9306)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9783..9803
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9900..10488
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9953..9973)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10371..10382)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10695..10928
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11057..11073)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11057..11073)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11070..11092)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11081..11097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11105..11111,11112..11129,11130..11135))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11150..11171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11288..11305)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11459..12047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11539..11558)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12218..13009,13010..13078))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12841..12860
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13079..13183)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13231..13365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13268..13287)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13322..13341
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
       61 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
      121 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
      181 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
      241 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
      301 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
      361 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
      421 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
      481 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      541 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      601 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
      661 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
      721 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
      781 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
      841 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
      901 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
      961 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1021 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1081 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1141 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1201 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1261 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1321 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1381 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1441 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1501 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1561 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1621 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1681 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1741 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1801 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1861 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1921 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1981 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcct
     2041 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2101 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2161 tttattacag ggacagcaga gatccagttt atcgatatcg aattcgtgag gaccgccatg
     2221 ttggcattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     2281 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2341 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2401 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2461 tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc
     2521 ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt
     2581 attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata
     2641 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2701 ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca
     2761 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
     2821 tcagatcact agaagcttta ttgcggtagt ttatcacagt taaattgcta acgcagtcag
     2881 tgcttctgac acaacagtct cgaacttaag ctgcagaagt tggtcgtgag gcactgggca
     2941 ggtaagtatc aaggttacaa gacaggttta aggagaccaa tagaaactgg gcttgtcgag
     3001 acagagaaga ctcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc
     3061 ctttctctcc acaggtgtcc actcccagtt caattacagc tcttaaggct agagtattaa
     3121 tacgactcac tatagggcta gcaaagcgat cgccatgaaa ctgcgtggag tcagcctggc
     3181 tgccggcttg ttcttactgg ccctgagtct ttgggggcag cctgcagagg ctgcggcttg
     3241 ctatgggtgt tctccaggat caaagtgtga ctgcagtggc ataaaagggg aaaagggaga
     3301 gagagggttt ccaggtttgg aaggacaccc aggattgcct ggatttccag gtccagaagg
     3361 gcctccgggg cctcggggac aaaagggtga tgatggaatt ccagggccac caggaccaaa
     3421 aggaatcaga ggtcctcctg gacttcctgg atttccaggg acaccaggtc ttcctggaat
     3481 gccaggccac gatggggccc caggacctca aggtattccc ggatgcaatg gaaccaaggg
     3541 agaacgtgga tttccaggca gtcccggttt tcctggttta cagggtcctc caggaccccc
     3601 tgggatccca ggtatgaagg gtgaaccagg tagtataatt atgtcatcac tgccaggacc
     3661 aaagggtaat ccaggatatc caggtcctcc tggaatacaa ggcctacctg gtcccactgg
     3721 tataccaggg ccaattggtc ccccaggacc accaggtttg atgggccctc ctggtccacc
     3781 aggacttcca ggacctaagg ggaatatggg cttaaatttc cagggaccca aaggtgaaaa
     3841 aggtgagcaa ggtcttcagg gcccacctgg gccacctggg cagatcagtg aacagaaaag
     3901 accaattgat gtagagtttc agaaaggaga tcagggactt cctggtgacc gagggcctcc
     3961 tggacctcca gggatacgtg gtcctccagg tcccccaggt ggtgagaaag gtgagaaggg
     4021 tgagcaagga gagccaggca aaagaggtaa accaggcaaa gatggagaaa atggccaacc
     4081 aggaattcct ggtttgcctg gtgatcctgg ttaccctggt gaacccggaa gggatggtga
     4141 aaagggccaa aaaggtgaca ctggcccacc tggacctcct ggacttgtaa ttcctagacc
     4201 tgggactggt ataactatag gagaaaaagg aaacattggg ttgcctgggt tgcctggaga
     4261 aaaaggagag cgaggatttc ctggaataca gggtccacct ggccttcctg gacctccagg
     4321 ggctgcagtt atgggtcctc ctggccctcc tggatttcct ggagaaaggg gtcagaaagg
     4381 tgatgaagga ccacctggaa tttccattcc tggacctcct ggacttgacg gacagcctgg
     4441 ggctcctggg cttccagggc ctcctggccc tgctggccct cacattcctc ctagtgatga
     4501 gatatgtgaa ccaggccctc caggcccccc aggatctcca ggtgataaag gactccaagg
     4561 agaacaagga gtgaaaggtg acaaaggtga cacttgcttc aactgcattg gaactggtat
     4621 ttcagggcct ccaggtcaac ctggtttgcc aggtctccca ggtcctccag gatctcttgg
     4681 tttccctgga cagaaagggg aaaaaggaca agctggtgca actggtccca aaggattacc
     4741 aggcattcca ggagctccag gtgctccagg ctttcctgga tctaaaggtg aacctggtga
     4801 tatcctcact tttccaggaa tgaagggtga caaaggagag ttgggttccc ctggagctcc
     4861 agggcttcct ggtttacctg gcactcctgg acaggatgga ttgccagggc ttcctggccc
     4921 gaaaggagag cctggtggaa ttacttttaa gggtgaaaga ggtccccctg ggaacccagg
     4981 tttaccaggc ctcccaggga atatagggcc tatgggtccc cctggtttcg gccctccagg
     5041 cccagtaggt gaaaaaggca tacaaggtgt ggcaggaaat ccaggccagc caggaatacc
     5101 aggtcctaaa ggggatccag gtcagactat aacccagccg gggaagcctg gcttgcctgg
     5161 taacccaggc agagatggtg atgtaggtct tccaggtgac cctggacttc cagggcaacc
     5221 aggcttgcca gggatacctg gtagcaaagg agaaccaggt atccctggaa ttgggcttcc
     5281 tggaccacct ggtcccaaag gctttcctgg aattccagga cctccaggag cacctgggac
     5341 acctggaaga attggtctag aaggccctcc tgggccaccc ggctttccag gaccaaaggg
     5401 tgaaccagga tttgcattac ctgggccacc tgggccacca ggacttccag gtttcaaagg
     5461 agcacttggt ccaaaaggtg atcgtggttt cccaggacct ccgggtcctc caggacgcac
     5521 tggcttagat gggctccctg gaccaaaagg tgatgttgga ccaaatggac aacctggacc
     5581 aatgggacct cctgggctgc caggaatagg tgttcaggga ccaccaggac caccagggat
     5641 tcctgggcca ataggtcaac ctggtttaca tggaatacca ggagagaagg gggatccagg
     5701 acctcctgga cttgatgttc caggaccccc aggtgaaaga ggcagtccag ggatccccgg
     5761 agcacctggt cctataggac ctccaggatc accagggctt ccaggaaaag caggtgcctc
     5821 tggatttcca ggtaccaaag gtgaaatggg tatgatggga cctccaggcc caccaggacc
     5881 tttgggaatt cctggcagga gtggtgtacc tggtcttaaa ggtgatgatg gcttgcaggg
     5941 tcagccagga cttcctggcc ctacaggaga aaaaggtagt aaaggagagc ctggccttcc
     6001 aggccctcct ggaccaatgg atccaaatct tctgggctca aaaggagaga agggggaacc
     6061 tggcttacca ggtatacctg gagtttcagg gccaaaaggt tatcagggtt tgcctggaga
     6121 cccagggcaa cctggactga gtggacaacc tggattacca ggaccaccag gtcccaaagg
     6181 taaccctggt ctccctggac agccaggtct tataggacct cctggactta aaggaaccat
     6241 cggtgatatg ggttttccag ggcctcaggg tgtggaaggg cctcctggac cttctggagt
     6301 tcctggacaa cctggctccc caggattacc tggacagaaa ggcgacaaag gtgatcctgg
     6361 tatttcaagc attggtcttc caggtcttcc tggtccaaag ggtgagcctg gtctgcctgg
     6421 atacccaggg aaccctggta tcaaaggttc tgtgggagat cctggtttgc ccggattacc
     6481 aggaacccct ggagcaaaag gacaaccagg ccttcctgga ttcccaggaa ccccaggccc
     6541 tcctggacca aaaggtatta gtggccctcc tgggaacccc ggccttccag gagaacctgg
     6601 tcctgtaggt ggtggaggtc atcctgggca accagggcct ccaggcgaaa aaggcaaacc
     6661 cggtcaagat ggtattcctg gaccagctgg acagaagggt gaaccaggtc aaccaggctt
     6721 tggaaaccca ggaccgcctg gacttccagg actttctggc caaaagggtg atggaggatt
     6781 acctgggatt ccaggaaatc ctggccttcc aggtccaaag ggcgaaccag gctttcacgg
     6841 tttccctggt gtgcagggtc ccccaggccc tcctggttct ccgggtccag ctctggaagg
     6901 acctaaaggc aaccctgggc cccaaggtcc tcctgggaga ccaggtccta caggttttca
     6961 aggtctacca ggtccagaag gtcctccagg tctccctgga aatggaggta ttaaaggaga
     7021 gaagggaaat ccaggccaac ctgggctacc tggcttgcct ggtttgaaag gagatcaagg
     7081 accaccagga ctccagggta atcctggccg gccgggtctc aatggaatga aaggagatcc
     7141 tggtctccct ggtgttccag gattcccagg catgaaagga cccagtggag tacctggatc
     7201 agctggccct gagggggaac cgggacttat tggtcctcca ggtcctcctg gattacctgg
     7261 tccttcagga cagagtatca taattaaagg agatgctggt cctccaggaa tccctggcca
     7321 gcctgggcta aagggtctac caggacccca aggacctcaa ggcttaccag gtccaactgg
     7381 ccctccagga gatcctggac gcaatggact ccctggcttt gatggtgcag gagggcgcaa
     7441 aggagaccca ggtctgccag gacagccagg tacccgtggt ttggatggtc cccctggtcc
     7501 agatggattg caaggtcccc caggtccccc tggaacctcc tctgttgcac atggatttct
     7561 tattacacgc cacagccaga caacggatgc accacaatgc ccacagggaa cacttcaggt
     7621 ctatgaaggc ttttctctcc tgtatgtaca aggaaataaa agagcccacg gtcaagactt
     7681 ggggacggct ggcagctgcc ttcgtcgctt tagtaccatg cctttcatgt tctgcaacat
     7741 caataatgtt tgcaactttg cttcaagaaa tgactattct tactggctct ctaccccaga
     7801 gcccatgcca atgagcatgc aacccctaaa gggccagagc atccagccat tcattagtcg
     7861 atgtgcagta tgtgaagctc cagctgtggt gatcgcagtt cacagtcaga cgatccagat
     7921 tccccattgt cctcagggat gggattctct gtggattggt tattccttca tgatgcatac
     7981 aagtgcaggg gcagaaggct caggtcaagc cctagcctcc cctggttcct gcttggaaga
     8041 gtttcgttca gctcccttca tcgaatgtca tgggaggggt acctgtaact actatgccaa
     8101 ctcctacagc ttttggctgg caactgtaga tgtgtcagac atgttcagta aacctcagtc
     8161 agaaacgctg aaagcaggag acttgaggac acgaattagc tgatgtcaag tgtgcatgaa
     8221 gaggacattc tcgagcggcg tcttcacact cgaagatttc gttggggact ggcgacagac
     8281 agccggctac aacctggacc aagtccttga acagggaggt gtgtccagtt tgtttcagaa
     8341 tctcggggtg tccgtaactc cgatccaaag gattgtcctg agcggtgaaa atgggctgaa
     8401 gatcgacatc catgtcatca tcccgtatga aggtctgagc ggcgaccaaa tgggccagat
     8461 cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat cactttaagg tgatcctgca
     8521 ctatggcaca ctggtaatcg acggggttac gccgaacatg atcgactatt tcggacggcc
     8581 gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact gtaacaggga ccctgtggaa
     8641 cggcaacaaa attatcgacg agcgcctgat caaccccgac ggctccctgc tgttccgagt
     8701 aaccatcaac ggagtgaccg gctggcggct gtgcgaacgc attctggcgt aaggatccaa
     8761 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     8821 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     8881 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     8941 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     9001 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     9061 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     9121 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     9181 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     9241 tcctcttcct catctccggg cctttcgacc tgcagttaac gccgccatga ccgagtacaa
     9301 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
     9361 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
     9421 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     9481 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     9541 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     9601 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     9661 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     9721 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     9781 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     9841 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctagacgc gtctggaaca
     9901 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     9961 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
    10021 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
    10081 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
    10141 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
    10201 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
    10261 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
    10321 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
    10381 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
    10441 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt
    10501 ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
    10561 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
    10621 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
    10681 agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
    10741 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
    10801 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
    10861 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
    10921 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
    10981 aatatcagag agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga
    11041 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    11101 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    11161 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    11221 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    11281 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    11341 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    11401 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    11461 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    11521 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    11581 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    11641 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    11701 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    11761 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    11821 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    11881 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    11941 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    12001 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    12061 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    12121 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    12181 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    12241 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    12301 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    12361 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    12421 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    12481 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    12541 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    12601 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    12661 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    12721 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    12781 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    12841 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    12901 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    12961 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    13021 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    13081 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    13141 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    13201 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
    13261 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    13321 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    13381 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    13441 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    13501 atttgttaaa tatgtactac
//
