LOCUS       sequence-389653-       10980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express hCOL4A5_Q1016X in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Omachi K, Kai H, Roberge M, Miner JH
  TITLE     NanoLuc reporters identify COL4A5 nonsense mutations susceptible to 
            drug-induced stop codon readthrough.
  JOURNAL   iScience. 2022 Feb 8;25(3):103891. doi: 10.1016/j.isci.2022.103891. 
            eCollection 2022 Mar 18.
  PUBMED    35243249
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-02-8"; volume: "25(3):103891. doi"; pages: " 
            10.1016/j.isci.2022.103891. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pNLF1-C_hCOL4A5-Nluc_Q1016X
FEATURES             Location/Qualifiers
     source          1..10980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        124..503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        504..707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     658..678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          843..975
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     958..977
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1019..1038
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1019..1037
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6141..6653
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(6729..6747)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    6784..6905
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6795..6814)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6849..6868
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7150..7507
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7150..7341
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7173..7193)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7358..7493
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7538..8575
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             8554..8562
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    8599..8647
                     /note="synthetic polyadenylation signal"
     promoter        8803..8907
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8908..8976,8977..9768)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9126..9145)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9926..10514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10006..10025)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10630..10913
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tagccatatt attcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
       61 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgccat
      121 gttggcattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
      181 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      241 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      301 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      361 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      421 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      481 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      541 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      601 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      661 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
      721 gtcagatcac tagaagcttt attgcggtag tttatcacag ttaaattgct aacgcagtca
      781 gtgcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga ggcactgggc
      841 aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg ggcttgtcga
      901 gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac atccactttg
      961 cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc tagagtatta
     1021 atacgactca ctatagggct agcaaagcga tcgccatgaa actgcgtgga gtcagcctgg
     1081 ctgccggctt gttcttactg gccctgagtc tttgggggca gcctgcagag gctgcggctt
     1141 gctatgggtg ttctccagga tcaaagtgtg actgcagtgg cataaaaggg gaaaagggag
     1201 agagagggtt tccaggtttg gaaggacacc caggattgcc tggatttcca ggtccagaag
     1261 ggcctccggg gcctcgggga caaaagggtg atgatggaat tccagggcca ccaggaccaa
     1321 aaggaatcag aggtcctcct ggacttcctg gatttccagg gacaccaggt cttcctggaa
     1381 tgccaggcca cgatggggcc ccaggacctc aaggtattcc cggatgcaat ggaaccaagg
     1441 gagaacgtgg atttccaggc agtcccggtt ttcctggttt acagggtcct ccaggacccc
     1501 ctgggatccc aggtatgaag ggtgaaccag gtagtataat tatgtcatca ctgccaggac
     1561 caaagggtaa tccaggatat ccaggtcctc ctggaataca aggcctacct ggtcccactg
     1621 gtataccagg gccaattggt cccccaggac caccaggttt gatgggccct cctggtccac
     1681 caggacttcc aggacctaag gggaatatgg gcttaaattt ccagggaccc aaaggtgaaa
     1741 aaggtgagca aggtcttcag ggcccacctg ggccacctgg gcagatcagt gaacagaaaa
     1801 gaccaattga tgtagagttt cagaaaggag atcagggact tcctggtgac cgagggcctc
     1861 ctggacctcc agggatacgt ggtcctccag gtcccccagg tggtgagaaa ggtgagaagg
     1921 gtgagcaagg agagccaggc aaaagaggta aaccaggcaa agatggagaa aatggccaac
     1981 caggaattcc tggtttgcct ggtgatcctg gttaccctgg tgaacccgga agggatggtg
     2041 aaaagggcca aaaaggtgac actggcccac ctggacctcc tggacttgta attcctagac
     2101 ctgggactgg tataactata ggagaaaaag gaaacattgg gttgcctggg ttgcctggag
     2161 aaaaaggaga gcgaggattt cctggaatac agggtccacc tggccttcct ggacctccag
     2221 gggctgcagt tatgggtcct cctggccctc ctggatttcc tggagaaagg ggtcagaaag
     2281 gtgatgaagg accacctgga atttccattc ctggacctcc tggacttgac ggacagcctg
     2341 gggctcctgg gcttccaggg cctcctggcc ctgctggccc tcacattcct cctagtgatg
     2401 agatatgtga accaggccct ccaggccccc caggatctcc aggtgataaa ggactccaag
     2461 gagaacaagg agtgaaaggt gacaaaggtg acacttgctt caactgcatt ggaactggta
     2521 tttcagggcc tccaggtcaa cctggtttgc caggtctccc aggtcctcca ggatctcttg
     2581 gtttccctgg acagaaaggg gaaaaaggac aagctggtgc aactggtccc aaaggattac
     2641 caggcattcc aggagctcca ggtgctccag gctttcctgg atctaaaggt gaacctggtg
     2701 atatcctcac ttttccagga atgaagggtg acaaaggaga gttgggttcc cctggagctc
     2761 cagggcttcc tggtttacct ggcactcctg gacaggatgg attgccaggg cttcctggcc
     2821 cgaaaggaga gcctggtgga attactttta agggtgaaag aggtccccct gggaacccag
     2881 gtttaccagg cctcccaggg aatatagggc ctatgggtcc ccctggtttc ggccctccag
     2941 gcccagtagg tgaaaaaggc atacaaggtg tggcaggaaa tccaggccag ccaggaatac
     3001 caggtcctaa aggggatcca ggtcagacta taacccagcc ggggaagcct ggcttgcctg
     3061 gtaacccagg cagagatggt gatgtaggtc ttccaggtga ccctggactt ccagggcaac
     3121 caggcttgcc agggatacct ggtagcaaag gagaaccagg tatccctgga attgggcttc
     3181 ctggaccacc tggtcccaaa ggctttcctg gaattccagg acctccagga gcacctggga
     3241 cacctggaag aattggtcta gaaggccctc ctgggccacc cggctttcca ggaccaaagg
     3301 gtgaaccagg atttgcatta cctgggccac ctgggccacc aggacttcca ggtttcaaag
     3361 gagcacttgg tccaaaaggt gatcgtggtt tcccaggacc tccgggtcct ccaggacgca
     3421 ctggcttaga tgggctccct ggaccaaaag gtgatgttgg accaaatgga caacctggac
     3481 caatgggacc tcctgggctg ccaggaatag gtgttcaggg accaccagga ccaccaggga
     3541 ttcctgggcc aataggtcaa cctggtttac atggaatacc aggagagaag ggggatccag
     3601 gacctcctgg acttgatgtt ccaggacccc caggtgaaag aggcagtcca gggatccccg
     3661 gagcacctgg tcctatagga cctccaggat caccagggct tccaggaaaa gcaggtgcct
     3721 ctggatttcc aggtaccaaa ggtgaaatgg gtatgatggg acctccaggc ccaccaggac
     3781 ctttgggaat tcctggcagg agtggtgtac ctggtcttaa aggtgatgat ggcttgcagg
     3841 gtcagccagg acttcctggc cctacaggag aaaaaggtag taaaggagag cctggccttc
     3901 caggccctcc tggaccaatg gatccaaatc ttctgggctc aaaaggagag aagggggaac
     3961 ctggcttacc aggtatacct ggagtttcag ggccaaaagg ttatcagggt ttgcctggag
     4021 acccagggca acctggactg agtggacaac ctggattacc aggaccacca ggtcccaaag
     4081 gtaaccctgg tctccctgga tagccaggtc ttataggacc tcctggactt aaaggaacca
     4141 tcggtgatat gggttttcca gggcctcagg gtgtggaagg gcctcctgga ccttctggag
     4201 ttcctggaca acctggctcc ccaggattac ctggacagaa aggcgacaaa ggtgatcctg
     4261 gtatttcaag cattggtctt ccaggtcttc ctggtccaaa gggtgagcct ggtctgcctg
     4321 gatacccagg gaaccctggt atcaaaggtt ctgtgggaga tcctggtttg cccggattac
     4381 caggaacccc tggagcaaaa ggacaaccag gccttcctgg attcccagga accccaggcc
     4441 ctcctggacc aaaaggtatt agtggccctc ctgggaaccc cggccttcca ggagaacctg
     4501 gtcctgtagg tggtggaggt catcctgggc aaccagggcc tccaggcgaa aaaggcaaac
     4561 ccggtcaaga tggtattcct ggaccagctg gacagaaggg tgaaccaggt caaccaggct
     4621 ttggaaaccc aggaccgcct ggacttccag gactttctgg ccaaaagggt gatggaggat
     4681 tacctgggat tccaggaaat cctggccttc caggtccaaa gggcgaacca ggctttcacg
     4741 gtttccctgg tgtgcagggt cccccaggcc ctcctggttc tccgggtcca gctctggaag
     4801 gacctaaagg caaccctggg ccccaaggtc ctcctgggag accaggtcct acaggttttc
     4861 aaggtctacc aggtccagaa ggtcctccag gtctccctgg aaatggaggt attaaaggag
     4921 agaagggaaa tccaggccaa cctgggctac ctggcttgcc tggtttgaaa ggagatcaag
     4981 gaccaccagg actccagggt aatcctggcc ggccgggtct caatggaatg aaaggagatc
     5041 ctggtctccc tggtgttcca ggattcccag gcatgaaagg acccagtgga gtacctggat
     5101 cagctggccc tgagggggaa ccgggactta ttggtcctcc aggtcctcct ggattacctg
     5161 gtccttcagg acagagtatc ataattaaag gagatgctgg tcctccagga atccctggcc
     5221 agcctgggct aaagggtcta ccaggacccc aaggacctca aggcttacca ggtccaactg
     5281 gccctccagg agatcctgga cgcaatggac tccctggctt tgatggtgca ggagggcgca
     5341 aaggagaccc aggtctgcca ggacagccag gtacccgtgg tttggatggt ccccctggtc
     5401 cagatggatt gcaaggtccc ccaggtcccc ctggaacctc ctctgttgca catggatttc
     5461 ttattacacg ccacagccag acaacggatg caccacaatg cccacaggga acacttcagg
     5521 tctatgaagg cttttctctc ctgtatgtac aaggaaataa aagagcccac ggtcaagact
     5581 tggggacggc tggcagctgc cttcgtcgct ttagtaccat gcctttcatg ttctgcaaca
     5641 tcaataatgt ttgcaacttt gcttcaagaa atgactattc ttactggctc tctaccccag
     5701 agcccatgcc aatgagcatg caacccctaa agggccagag catccagcca ttcattagtc
     5761 gatgtgcagt atgtgaagct ccagctgtgg tgatcgcagt tcacagtcag acgatccaga
     5821 ttccccattg tcctcaggga tgggattctc tgtggattgg ttattccttc atgatgcata
     5881 caagtgcagg ggcagaaggc tcaggtcaag ccctagcctc ccctggttcc tgcttggaag
     5941 agtttcgttc agctcccttc atcgaatgtc atgggagggg tacctgtaac tactatgcca
     6001 actcctacag cttttggctg gcaactgtag atgtgtcaga catgttcagt aaacctcagt
     6061 cagaaacgct gaaagcagga gacttgagga cacgaattag ccgatgtcaa gtgtgcatga
     6121 agaggacatt ctcgagcggc gtcttcacac tcgaagattt cgttggggac tggcgacaga
     6181 cagccggcta caacctggac caagtccttg aacagggagg tgtgtccagt ttgtttcaga
     6241 atctcggggt gtccgtaact ccgatccaaa ggattgtcct gagcggtgaa aatgggctga
     6301 agatcgacat ccatgtcatc atcccgtatg aaggtctgag cggcgaccaa atgggccaga
     6361 tcgaaaaaat ttttaaggtg gtgtaccctg tggatgatca tcactttaag gtgatcctgc
     6421 actatggcac actggtaatc gacggggtta cgccgaacat gatcgactat ttcggacggc
     6481 cgtatgaagg catcgccgtg ttcgacggca aaaagatcac tgtaacaggg accctgtgga
     6541 acggcaacaa aattatcgac gagcgcctga tcaaccccga cggctccctg ctgttccgag
     6601 taaccatcaa cggagtgacc ggctggcggc tgtgcgaacg cattctggcg taaggccgcg
     6661 actctagggc atgcaagctg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     6721 tgctgccacc gctgagcaat aactagcata accccttggg gcggccgctt cgagcagaca
     6781 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     6841 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     6901 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg
     6961 tttttttaag caagtaaaac ctctacaaat gtggtaaaat cgaattttaa caaaatatta
     7021 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     7081 gcatacgcgg atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt
     7141 aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     7201 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7261 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7321 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7381 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7441 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7501 ttgcaaaaag ctcgattctt ctgacactag cgccaccatg aagaagcccg aactcaccgc
     7561 taccagcgtt gaaaaatttc tcatcgagaa gttcgacagt gtgagcgacc tgatgcagtt
     7621 gtcggagggc gaagagagcc gagccttcag cttcgatgtc ggcggacgcg gctatgtact
     7681 gcgggtgaat agctgcgctg atggcttcta caaagaccgc tacgtgtacc gccacttcgc
     7741 cagcgctgca ctacccatcc ccgaagtgtt ggacatcggc gagttcagcg agagcctgac
     7801 atactgcatc agtagacgcg cccaaggcgt tactctccaa gacctccccg aaacagagct
     7861 gcctgctgtg ttacagcctg tcgccgaagc tatggatgct attgccgccg ccgacctcag
     7921 tcaaaccagc ggcttcggcc cattcgggcc ccaaggcatc ggccagtaca caacctggcg
     7981 ggatttcatt tgcgccattg ctgatcccca tgtctaccac tggcagaccg tgatggacga
     8041 caccgtgtcc gccagcgtag ctcaagccct ggacgaactg atgctgtggg ccgaagactg
     8101 tcccgaggtg cgccacctcg tccatgccga cttcggcagc aacaacgtcc tgaccgacaa
     8161 cggccgcatc accgccgtaa tcgactggtc cgaagctatg ttcggggaca gtcagtacga
     8221 ggtggccaac atcttcttct ggcggccctg gctggcttgc atggagcagc agactcgcta
     8281 cttcgagcgc cggcatcccg agctggccgg cagccctcgt ctgcgagcct acatgctgcg
     8341 catcggcctg gatcagctct accagagcct cgtggacggc aacttcgacg atgctgcctg
     8401 ggctcaaggc cgctgcgatg ccatcgtccg cagcggggcc ggcaccgtcg gtcgcacaca
     8461 aatcgctcgc cggagcgcag ccgtatggac cgacggctgc gtcgaggtgc tggccgacag
     8521 cggcaaccgc cggcccagta cacgaccgcg cgctaaggag gtaggtcgag tttagactct
     8581 agccatcacg atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     8641 ttgtgtgaat cgatagcgat aaggatcctc tttgcgcttg cgttttccct tgtccagata
     8701 gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc tttctacgta
     8761 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8821 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8881 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8941 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     9001 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     9061 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cactttcaaa
     9121 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     9181 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     9241 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     9301 gcggccaact tacttctgac aactatcgga ggaccgaagg agctaaccgc ttttttgcac
     9361 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     9421 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     9481 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     9541 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     9601 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     9661 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9721 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaatt cgaaatgacc
     9781 gaccaagcga cgcccaaccg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9841 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9901 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9961 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    10021 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10081 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10141 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10201 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10261 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10321 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    10381 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10441 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10501 aaaaaggatt tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10561 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10621 ttttatagtc cggaaataca ggaacgcacg ctggatggcc cttcgctggg atggtgaaac
    10681 catgaaaaat ggcagcttca gtggattaag tgggggtaat gtggcctgta ccctctggtt
    10741 gcataggtat tcatacggtt aaaatttatc aggcgcgatt gcggcagttt ttcgggtggt
    10801 ttgttgccat ttttacctgt ctgctgccgt gatcgcgctg aacgcgtttt agcggtgcgt
    10861 acaattaagg gattatggta aatccactta ctgtctgccc tcgtagccat cgagataaac
    10921 cgcagtactc cggccacgat gcgtccggcg tagaggatcg agatcttcaa tattggccat
//
