LOCUS       sequence-389660-        9510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' 
            XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains 
            FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Truong DJ, Armbrust N, Geilenkeuser J, Lederer EM, Santl TH, Beyer 
            M, Ittermann S, Steinmassl E, Dyka M, Raffl G, Phlairaharn T, 
            Greisle T, Zivanic M, Grosch M, Drukker M, Westmeyer GG
  TITLE     Intron-encoded cistronic transcripts for minimally invasive 
            monitoring of coding and non-coding RNAs.
  JOURNAL   Nat Cell Biol. 2022 Nov;24(11):1666-1676. doi: 
            10.1038/s41556-022-00998-6. Epub 2022 Nov 7.
  PUBMED    36344775
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-022-00998-6"; journalName: "Nat Cell Biol"; date: 
            "2022-11"; volume: "24"; issue: "11"; pages: "1666-1676"
COMMENT     Sequence Label: pINSPECT_5'-xrRNA_NLuc_3'-XAP1
FEATURES             Location/Qualifiers
     source          1..9510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1064..1097
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1168..1201
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     polyA_signal    1800..1921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1837..1856)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1891..1910
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1953..1972
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1971..2026
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    complement(2133..2166)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2237..2270)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(2319..2375)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3510..3566)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     4153..4172
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(4199..5381)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4208..5150
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4231..4251)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    join(5457..6031,6032..6034,6035..6043)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5625..5642)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5852..5871
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6568..6587)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     primer_bind     complement(7322..7342)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7322..7340)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7361..7377)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7361..7377)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7374..7396)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7385..7401
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7409..7415,7416..7433,7434..7439))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7454..7475
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7592..7609)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7763..8351)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7843..7862)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8522..9313,9314..9382))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9145..9164
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9383..9487)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggaggtctc
      661 tgtaagtatc ctttttacag cacaacttaa tgagacagat agaaactggt cttgtagaaa
      721 cagagtagac acttgctttt gacacaactg tgtttacttg caatccccca aaacagacag
      781 atagtcaggc cagccggtta ggctgccacc gaaggttggt agacggtgct gcctgcgacc
      841 aaccccagga ggactgggtt accaaagctg attctccacg gttggaaagc ctcccagaac
      901 cgtctcggaa gaggagtccc tgccaacaat ggagatgaag cccgtgtcag atcgcgaaag
      961 cgccacttcg ccgaggagtg caatctgtga ggccccagga ggactgggta aacagtcgac
     1021 ccgggcggcc gcattttatc gaattgcaac ctcttcgagg gacataactt cgtataatgt
     1081 atgctatacg aagttatggg tcgatgctga gatctggact agagggtcga tggtgatgct
     1141 tggatcaaaa taggaagaat cccaatgata acttcgtata aagtatccta tacgaagtta
     1201 tactagaatt gcgtaccgcc accatgtcgc caaggccacc tcccctgtga gctagactgg
     1261 acagccaatg acgggtaaga gagtgacatt tctcactaac ctaagacagg agggccgtca
     1321 aagctactgc ctaatccaat gacgggtaat agtgacaaga aatgtatcac tccaacctaa
     1381 gacaggcgca gcctccgagg gatgtgtctt ttgtttttta taattaaaaa gggtgacacg
     1441 tgaggccacc tcccctgtga gctagactgg acagccaatg acgggtaaga gagtgacatt
     1501 tctcactaac ctaagacagg agggccgtca aagctactgc ctaatccaat gacgggtaat
     1561 agtgacaaga aatgtatcac tccaacctaa gacaggcgca gcctccgagg gatgtgtctt
     1621 ttgtttttta taattaaaaa gggtgaagcc gacacacaaa aaaccaacac acagatctct
     1681 agagctctgg atcttttatt gcccgggcta ccacatttgt agaggtttta cttgctttaa
     1741 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     1801 acttgtttat tgcagcttat aatggttaca aatatagcaa tagcatcaca aatttcacaa
     1861 atatagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     1921 atcatgtctg tatactttaa atgatttgcc ctcccatatg tccttccgag tgagagacac
     1981 aaaaaattcc aacacactat tgcaatgaaa atacatttcc tttattagcc agaagtcaga
     2041 tgccagcaca acaaccagca cgttgcccag gagctgtagg aaaaagaaga aggcatgaac
     2101 atagttagca gagttacgta gcgatcgcta gtataacttc gtatagcata cattatacga
     2161 agttatgggt cgatggtgac aatagcgaaa aagaaagaac aatcaagggt ccccaaactc
     2221 acctaggtcc tttgatataa cttcgtatag gatactttat acgaagttat ggcatctcct
     2281 tcggaagttc ctattctatt tgaagtatag gaacttccag gtccaggatt ttcctcgacg
     2341 tcccctgcct gcttgagcag tgaaaagtta gttgccccgg atccgttggc ttcgcccatc
     2401 tctctagcga aggttctggc gaggtcacag atggtgggga tgctgccagg ggttgtcaca
     2461 tgggtctgca ccatgccgct tgtcagttgc agcagggcat ctctgcatcc ggctgggctc
     2521 tgatcgtagt ccaggatgaa cacatgcatg ggccgcagtc tttttgccag cacgtcgaga
     2581 gcccaggcga acacgttata caggtcgccg ttaggggcca gcagttcagg ggctctgaac
     2641 agtgtgaaca gggtgtcgcc gatgtgaggt ctgggtccag cgttagactg aggctcagcg
     2701 ccttgtggag gaactgctgt gccagagagc tgtccccagt cctctctcca ggaaccgccg
     2761 ccttgaagat agcgcacggt gttagccagc aggccgtaca ctcttctgat ggcggccagc
     2821 atagccagat ccagtctctc gccaggcctc tgtctcttag ccagtctgtc gatgtgtctg
     2881 tcctctggca gggctccaag cacgatgttt gtgccgggca gtgtaggagg aatcagagcc
     2941 acgaaagcca gcacggcctg aggtgtcatg ctgcccatca gataccgagc ggcagggtaa
     3001 cacagcaggg cgaagatggg gtgtctgtcg aagatcaggg tcagagcagg tggtggagcg
     3061 tgagaagatc cagcctcgcc gccaatatgt ggagccagca cagcgtctgt cacggcgtaa
     3121 ggcatgccca ttgtgatctg ggcagatgtc atgaccacag cagcgtcgcc tgcgctaatt
     3181 tcgccctggt ccagtctgtg ctgtgtggtg tagatgttgg cgattgtctc agaggctccc
     3241 aggacctgcc agtaggtcat aggctcgggc acgtacacga tgtcatctct gctgcccaga
     3301 gccaccagca gctgtgttgt tgtggtcttg cccatgccat gagggccgtc gatatacact
     3361 ctcagcagtg tgggcatctt ctgttccagc cgcacttctg tagcctcttg ctgccttctt
     3421 ggcctgaggg ctgttcttct gttgctgtgg cctctgcttc tagcggcttg atcgaaggcg
     3481 ctggcgtgct ggtgacaagg ataagaagca ggaccggggt tctcttccac gtcgccagcc
     3541 tgtttcagca ggctgaagtt tgtagcgccg gatccggcgc caggctttct ggtcatgcac
     3601 caggttctag ggccctcagg cacttccacg tcggcggtca cggtgaagcc cagtctctcg
     3661 tagaagggca ggtttctggg ggcgcttgtt tccaggaagg cgggcacgcc agccctttca
     3721 gcagcttcca ccccaggcag caccacagca gatcccagtc ccttgccctg gtggtcaggt
     3781 gacacgccca cggtggccag aaaccaggca ggctcttttg gtctgtgggg ggccagcagg
     3841 ccttccatct gctgctgggc agccagtcta gagccgctca gctcggccat tctaggtccg
     3901 atctcggcga acacagcgcc ggcttccaca gactcagggg ttgtccacac agccacagcg
     3961 gcgccatcat cggccaccca cactttgccg atgtccaggc ccactctggt cagaaacagt
     4021 tcctgcagct cggtcactct ctcgatgtgc cggtcggggt ccacggtgtg tcttgtggca
     4081 gggtaatcgg cgaaggcagc ggccagtgtc cgcacagctc ttggcacatc gtccctggtg
     4141 gccagccgca ctgtgggctt gtactcggtc atggtggcgc gccttttagg ggtagttttc
     4201 acgacacctg aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc
     4261 aagatccaaa ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc
     4321 taacttcagt ctccacccac tcagtgtggg gaaactccat cgcataaaac ccctcccccc
     4381 aacctaaaga cgacgtactc caaaagctcg agaactaatc gaggtgcctg gacggcgccc
     4441 ggtactccgt ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg
     4501 tgggtgactc acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctccctgcag
     4561 cagggccggg aagcggccat ctttccgctc acgcaactgg tgccgaccgg gccagccttg
     4621 ccgcccaggg cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag
     4681 cttgagacta cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac
     4741 atgtgcgctg ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag
     4801 tgtgcagatc ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag
     4861 gtcatcaaaa attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt
     4921 gccaccagat tcgcacgcgg cggccccagc gcccaggcca ggcctcaact caagcacgag
     4981 gcgaaggggc tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct
     5041 cgggatcaag aatcacgtac tgcagccagg ggcgtggaag taattcaagg cacgcaaggg
     5101 ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg
     5161 gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct
     5221 cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg
     5281 cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt ctcggggact
     5341 gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc caattcgaag ttcctattct
     5401 atttgaagta taggaacttc aaatacaagg gcttactgtg cggactggga cgcccccccc
     5461 tctccctccc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     5521 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     5581 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     5641 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     5701 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     5761 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     5821 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     5881 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     5941 atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac
     6001 gtggttttcc tttgaaaaac acgatgataa tatggccacc accatggtct ttacactgga
     6061 agatttcgtc ggcgactggc ggcagacagc tggctacaat ctggaccagg tgctggaaca
     6121 aggcggcgtg tcctctctgt ttcaaaacct gggagtgtct gtgaccccta tccagagaat
     6181 cgtgctgagc ggcgagaacg gcctgaagat cgacatccat gtgatcatcc cttacgaggg
     6241 cctgtccggc gatcagatgg gccagatcga gaagattttc aaggtggtgt accccgtgga
     6301 cgaccaccac ttcaaagtga tcctgcacta cggcaccctg gtcatcgatg gcgtgacccc
     6361 taacatgatc gactacttcg gcagacccta cgagggaatc gccgtgttcg acggcaagaa
     6421 aatcaccgtg accggcacac tgtggaacgg caacaagatc atcgacgagc ggctgatcaa
     6481 ccccgatggc agcctgctgt tcagagtgac catcaacggc gtgacaggat ggcggctgtg
     6541 cgaaagaatc ctggcctaat gtacagatct ttttccctct gccaaaaatt atggggacat
     6601 catgaagccc ctgggttgca cacgtctcga gggacgggca gggctgggcg agacgtgtgc
     6661 aaccctgagc atctgacttc tggctaatat aggaaattta ttttcattgc aagtacagat
     6721 atcactatcg ataccgcggg gatcctctag agtagaccac ctcccctgcg agctaagctg
     6781 gacagccaat gacgactaag agagtgacat ttttcactaa gctaagacag gagggccgtc
     6841 agagctactg cctaatccaa agacggctaa aagtgataaa aatgtatcac tccaagttaa
     6901 gacaggcgca gcttccgagg gatttgcctg gccggattga ctgacgtctt aaccactgca
     6961 tatcgatacc gcggggatcc tctagagtag accacctccc ctgcgagcta agctggacag
     7021 ccaatgacga ctaagagagt gacatttttc actaagctaa gacaggaggg ccgtcagagc
     7081 tactgcctaa tccaaagacg gctaaaagtg ataaaaatgt atcactccaa gttaagacag
     7141 gcgcagcttc cgagggattt gcgtttaaac gcggccgctc tatactcgac gattgactat
     7201 ctgacaaatc atagagaaca tagagaacct cctcgagtcg cctgcttttc tgcctggtac
     7261 taacttctct cccctctcct cttttctttt tctgcaggga gaccgtaccc agcttttgtt
     7321 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7381 gaaattgtta tccgctcaca attccacaca acatacgagc cgggagcata aagtgtaaag
     7441 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7501 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7561 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7621 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7681 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7741 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7801 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7861 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7921 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7981 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8041 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8101 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggaggtgcta
     8161 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8221 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8281 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8341 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8401 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8461 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8521 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8581 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8641 ccagtgctgc aatgataccg cgagatccac gctcaccggc tccagattta tcagcaataa
     8701 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8761 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8821 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8881 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8941 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9001 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9061 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9121 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9181 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9241 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9301 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9361 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9421 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9481 ttccgcgcac atttccccga aaagtgccac
//
