LOCUS       sequence-389699-       10783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. 
            laidlawii. Contains the OriC from A. laidlawii 8195.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Nucifora DP, Mehta ND, Giguere DJ, Karas BJ
  TITLE     An Expanded Genetic Toolbox to Accelerate the Creation of 
            Acholeplasma laidlawii Driven by Synthetic Genomes.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):45-53. doi: 
            10.1021/acssynbio.3c00399. Epub 2023 Dec 19.
  PUBMED    38113213
REFERENCE   2  (bases 1 to 10783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00399"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "45-53"
COMMENT     Sequence Label: pAL1
FEATURES             Location/Qualifiers
     source          1..10783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    396..417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(534..551)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(705..1293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(785..804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1464..2255,2256..2324))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2087..2106
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2325..2429)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3086..3272
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             3273..3935
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     oriT            4349..4458
                     /note="incP origin of transfer"
     CDS             4491..4862
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     misc_feature    9209..9265
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(9266..9283)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9266..9282)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9275..9297)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9491..9510)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggcccgatc gccaacaaat actacctttt accttgctct tcctgctctc aggtattaat
       61 gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg tgattttaca
      121 ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt ctaataaata
      181 tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt ttttttcttc
      241 attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca aaacataaaa
      301 ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac gaggcgcgtg
      361 taagttacag gcaagcgatc cgcctggggt gcctaatgag tgagctaact cacattaatt
      421 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
      481 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
      541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1141 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     1501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     1561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     1621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     1681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     1741 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     1801 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     1861 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     1921 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     1981 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     2041 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     2101 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     2161 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     2221 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     2281 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     2341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     2401 tagaaaaata aacaaatagg ggttccgcgg gcccgatgga gataaggtta agctaaccgg
     2461 taaagttgta tttatatcaa agagtgtcaa gaaagttacc atcactcaaa accaatatga
     2521 aatagtaaaa cgttcagcga atgaacgcca atatatcaca tttgattttg ttaatttaaa
     2581 tgaagtacgc cttggtacac gtgtattttt aactgaagcg ggtcaatcag accgtgaaaa
     2641 acgtgatcac aaacgttctc ttcgcgaaca gaaaagccaa gcgcttgcag aaaaaaaagc
     2701 tgcagcaatt caaaaaaagg ctgataagcg catgagaaaa gaagaaaaac gcatatcaaa
     2761 aaaagataaa aataaaatgg acatgtttga tgaataattt agataggtgc ttaaatgtaa
     2821 gcacctattt ttattattag gttctatggt acaataaatt aagtaaacca atgaggaggc
     2881 aaaatgagaa aaatagcaag tttacttatg atagtaagta gtatctttct tttaagagtt
     2941 acgctaggga taacagggta atatagtaag agcttggtga gcgctaggag tcactgccag
     3001 gtatcgtttg aacacggcat tagtcaggga agtcataaca cagtcctttc ccgcaatttt
     3061 ctttttctat tactcttggc ctcctctagt acactctata tttttttatg cctcggtaat
     3121 gattttcatt ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat
     3181 tatcacataa tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa
     3241 aaaatgagca ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc
     3301 gtattacaaa tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga
     3361 tagagcactc gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat
     3421 cgcaagtgat taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg
     3481 ccaagcattc cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc
     3541 atcacaccac tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctagggg
     3601 ccgtgcgtgg agtaaaaagg tttggatcag gatttgcgcc tttggatgag gcactttcca
     3661 gagcggtggt agatctttcg aacaggccgt acgcagttgt cgaacttggt ttgcaaaggg
     3721 agaaagtagg agatctctct tgcgagatga tcccgcattt tcttgaaagc tttgcagagg
     3781 ctagcagaat taccctccac gttgattgtc tgcgaggcaa gaatgatcat caccgtagtg
     3841 agagtgcgtt caaggctctt gcggttgcca taagagaagc cacctcgccc aatggtacca
     3901 acgatgttcc ctccaccaaa ggtgttctta tgtagtgaca ccgattattt aaagctgcag
     3961 catacgatat atatacatgt gtatatatgt atacctatga atgtcagtaa gtatgtatac
     4021 gaacagtatg atactgaaga tgacaaggta atgcatcatt ctatacgtgt cattctgaac
     4081 gaggcgcgct ttcctatcac gtgctataaa aataattata atttaaattt tttaatataa
     4141 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
     4201 ccgaagatgt aaaatcttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt
     4261 cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact
     4321 ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga
     4381 gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg
     4441 ctctgcgagg ctggccggct accgccggcg taacagatga gggcaagcgg atggctgatg
     4501 aaaccaagcc aaccaggaag ggcagcccac ctatcaaggt gtactgcctt ccagacgaac
     4561 gaagagcgat tgaggaaaag gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg
     4621 ccgtcggcca gggctacaaa atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg
     4681 cccgcatcaa tggcgacctg ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg
     4741 acccgcgcac ggcgcggttc ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag
     4801 agaagcagga cgagcttggc aaggtcatga tgggcgtggt ccgcccgagg gcagagccat
     4861 gactttttta gccgctaaaa cggccggggg gtgcgcgtga ttgccaagca cgtccccatg
     4921 cgctccatca agaagaggca cttcgagctg taagtacatc accgacgagc aaggcaagac
     4981 gatcaatcaa aaaaccacct ttcttatgaa accttgcttt tcttattata aataaagtgt
     5041 acttaaaagt caaacataaa atggtctttc ttttatttat tttttatttg atttttccca
     5101 caatttttaa tatgaatgtt ccccacaatt attgtccaca cattgtggat aaagtttcca
     5161 cattttattc acaatgttga taagtagcgt aagtatattt aacagcctta caaagcaaat
     5221 gatacactga aagttatcca caatttaata tttaaagaac agctaaatca aaaagttatc
     5281 cacaaataat gtggaaaact ttttattaaa tttgtcgttt cttatgctat catagtttta
     5341 cataaattat taactcaggg aggcagtcat gagtccaaac agcacactat ggcagacaat
     5401 attacaggat ttagaaaaac tatacaacga ggagacttac aacgagctat ttctaccagt
     5461 gacttctact tttaaagatc aaaacggatt acttacaatg gttgtagcaa atgagttctt
     5521 aaagaatcgt atcaataaac tatacatcgc aaaaattaac gaacttgcta ctaaatattc
     5581 aagtactcca gttagattga aattcatatc acaagaggaa gttattgaag aaccagtagc
     5641 ggatcgtaaa ttaaccattg attatcgtca aggtaactta aactctacat atacctttga
     5701 ctcttttgtt gttggaaaat ctaacatgtt tgcttttcgt atggcgatga aggttgctga
     5761 tcaacctgca gcagtagcaa accccttcta catatttggt gatgtaggtt taggtaaaac
     5821 ccatcttatg caagcaatag gtaactatat attagataat gatgttgaaa aacgtatatt
     5881 atatgttaaa gctgataatt ttattgaaga ctttgtatca ttattatcaa gaaacaaaaa
     5941 taagactgaa gaattcaatg ctaaatataa agatatagat gttatattag tggatgatat
     6001 tcaaattatg gccaacgcta gtaaaactca aatggaattc tttaaactct ttgactatct
     6061 atatttaaat aataagcaaa ttgtcattac gtctgataaa ccagcttcac aattaacaaa
     6121 tattatgccg cgattaacaa cacgttttga agctggtctc tctgtagaca tacaaatacc
     6181 tgaattagaa catagaataa gcattttaaa gagaaaaaca gctacattag atgcaaactt
     6241 agaggtagga gaggatatct taacctttat tgcatctcaa tttgcagcaa atattagaga
     6301 aatggagggt gcactcattc gtttaattag ttatgcacaa acctttaatc tagaaataac
     6361 aatggatgtt gttgaagaag cacttggtgc tgtcttaaaa acaaagaaga aaacaaatca
     6421 attaaacgaa aataactacg ataagatcca aagtatcgtt gcagattact tccaagtgtc
     6481 attaccagac ttaattggta aaaaaagaca tgctaaattc acattaccta gacatatcgc
     6541 aatgtatctt atcaaactca aattcaatat accttataaa acgattggtt ctttgtttaa
     6601 tgatagagac cactctactg tattggctgc ttgtgagaaa gtagaacgcg atatgaggat
     6661 ggattcgaac ttaaagtttg ctgttgactc aattgtcaaa aaaatagatt caccatcatt
     6721 aaagtgataa atgtttataa aaatgattaa atgtggtaaa ataaatggta gatgaagcga
     6781 ttattgctag tttcccactt tcccacagac actaacaata acaaagaaga aataataatt
     6841 aataaaaggg gtaaaattcc ctttagtgag ggttaatgtc gtgactggga aaaccctggc
     6901 ggaattcggc cagtctacat gtactctttt tgataaaaaa ttggagattc ctttacaaat
     6961 atgctcttac gtgctattat ttaagtgact atttaaaagg agttaataaa tatgcggcaa
     7021 ggtattctta aataaactgt caatttgata gcgggaacaa ataattagat gtcctttttt
     7081 aggagggctt agttttttgt acccagttta agaatacctt tatcatgtga ttctaaagta
     7141 tccagagaat atctgtatgc tttgtatacc tatggttatg cataaaaatc ccggtgataa
     7201 aagtatttat cactgggatt tttatgccct tttgggtttt tgaatggagg aaatctagat
     7261 gaaaatcata aatatcggtg tattagctca cgttgatgca ggaaaaacaa cattaactga
     7321 atcactttta tataactctg gtgcaattac tgaacttggt tcagtagata aaggtactac
     7381 tcgtactgat aatacattat tagaacgtca acgtggaatc acaattcaaa caggtatcac
     7441 atcttttcaa tggaaaaata caaaagtaaa tattatagat acacctggac acatggattt
     7501 ccttgcagaa gtataccgta gtctttcagt attagatggt gctattttac ttatcagcgc
     7561 taaagatgga gttcaagctc aaactcgtat cttatttcac gcattacgta aaatgggtat
     7621 tccaacaatt ttctttataa acaaaattga ccaaaacgga attgatttaa gtacagttta
     7681 tcaagatatc aaagaaaaac tttctgctga aatcgttatt aaacaaaaag ttgaattata
     7741 cccaaacgtt tgcgtaacaa attttactga atcagaacaa tgggatacag ttatagaagg
     7801 taatgatgat ttattagaaa aatacatgtc aggtaaatca ttagaagcat tagaattaga
     7861 acaagaagaa agtattcgtt tccaaaactg ttctttattc cctttatacc atggaagcgc
     7921 taaaagtaac ataggtattg ataacttaat tgaagttatt actaacaaat tttactcttc
     7981 aactcatcgt ggtccttctg aattatgcgg taacgttttc aaaatcgaat atacaaaaaa
     8041 acgtcaacgt ttagcttata tacgtcttta tagtggtgtt ttacatttac gtgatagtgt
     8101 tcgtgttagt gaaaaagaaa agattaaagt tacagaaatg tatacttcta ttaacggtga
     8161 attatgcaaa attgaccgtg catattcagg tgaaattgta attttacaaa acgaatttct
     8221 taaacttaat agtgtacttg gtgacacaaa acttttacca caacgtaaga aaattgaaaa
     8281 tccacaccca ttacttcaaa caacagtaga accaagcaaa cctgaacaac gtgaaatgct
     8341 tttagatgct cttttagaaa ttagtgactc tgacccactt ttacgttact atgtagattc
     8401 tactactcat gaaattattc tttctttcct tggtaaagtt caaatggaag ttatttctgc
     8461 attattacaa gaaaaatatc atgttgaaat cgaattaaaa gaacctactg taatttatat
     8521 ggaacgtcca ttaaaaaatg ctgaatatac aattcatatt gaagttccac caaatccatt
     8581 ttgggcttct attggtcttt ctgtttctcc acttccactt ggtagcggaa tgcaatatga
     8641 aagtagcgta agtttaggtt atcttaatca aagtttccaa aacgcagtta tggaaggtat
     8701 tcgttacggt tgcgaacaag gtttatacgg ttggaatgtt acagactgca aaatctgttt
     8761 taagtatgga ctttactatt cacctgtatc aacacctgct gactttcgta tgcttgcacc
     8821 aattgtttta gaacaagttt taaagaaagc tggaactgaa cttttagaac catacctttc
     8881 ttttaaaatc tatgcaccac aagaatactt aagtcgtgct tataacgatg cacctaaata
     8941 ctgtgctaat attgttgata ctcaattaaa gaacaacgaa gtaattttaa gcggagaaat
     9001 tcctgcacgt tgtattcaag aatatcgtag tgatttaaca tttttcacta atggacgttc
     9061 tgtttgctta actgaattaa aaggttatca tgttactact ggtgaacctg tatgccaacc
     9121 acgtcgtcct aatagtcgta ttgataaagt tcgttatatg ttcaacaaaa tcacataata
     9181 atactagtag catgaccatg attacgccaa gcttgcatgc ctgcaggtcg actctagagg
     9241 atccccgggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     9301 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     9361 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     9421 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     9481 gcactctcag tacaatctgc tctgatgccg catagtattt tttgaattaa gtattaaata
     9541 agtgtaaaat atataatagt aaaaacgccc caaaaggggc agacaaaata gtagaaatat
     9601 atctattatt cttgatttgt tatagaaatt ttaaggagaa aaaaacatga ctgaatataa
     9661 acctactgtt agattagcta ctagagatga tgttcctaga gctgttagaa ctttagctgc
     9721 tgcttttgct gattatcctg ctactagaca tactgttgat cctgatagac atattgaaag
     9781 agttactgaa ttacaagaat tatttttaac tagagttggt ttagatattg gtaaagtttg
     9841 ggttgctgat gatggcgctg ctgttgctgt ttggactact cctgaaagtg ttgaagctgg
     9901 tgctgttttt gctgaaattg gtcctagaat ggctgaatta agtggtagta gattagctgc
     9961 tcaacaacaa atggaaggtt tattagctcc tcatagacct aaagaacctg cttggttttt
    10021 agctactgtt ggtgttagtc ctgatcatca aggtaaaggt ttaggtagtg ctgttgtttt
    10081 acctggtgtt gaagctgctg aaagagctgg tgttcctgct tttttagaaa ctagtgctcc
    10141 tagaaattta cctttttatg aaagattagg ttttactgtt actgctgatg ttgaagttcc
    10201 tgaaggtcct agaacttggt gtatgactag aaaacctggt gcttaacaaa aaaatcggga
    10261 aatcccgatt ttttattatt aaatatagat tttccggagt atgtagatgt ctactttaac
    10321 tagatgtcat tgggcaaatt caagtgaaaa catggcgcac tatcatgatc atgaatgggg
    10381 tgtacctgta tatgatgatt taaaactatt tcaaaagtta cttttagaca tgcaacaagc
    10441 aggactgtct tggtcaattg ttttaaacaa gagaaaagat attttagaat catttgataa
    10501 ctttgatcca tacatcatga taagttatga tgatgaaaaa ataaatagtt tactaaataa
    10561 ccctaagatt attagaaata ggcttaaaat agaagctatg attcataatg caaaaatgta
    10621 tataaagcat tttggacaaa cagtaagttt ttcaaacttt ttatggtcct atgtggacca
    10681 taaagtagtc gtacacgaca ttaaagtacc taaagataca ccaactgaaa cagatatatc
    10741 taaacgtatc agtaaagatt taaaaagatt aggatttaaa ttt
//
