LOCUS       sequence-389719-        9398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rep/Cap plasmid for the production of M.Fas.4, an AAV capsid with 
            CNS tropism in macaques..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Stanton AC, Lagerborg KA, Tellez L, Krunnfusz A, King EM, Ye S, 
            Solomon IH, Tabebordbar M, Sabeti PC
  TITLE     Systemic administration of novel engineered AAV capsids facilitates 
            enhanced transgene expression in the macaque CNS.
  JOURNAL   Med (N Y). 2022 Nov 15:S2666-6340(22)00456-1. doi: 
            10.1016/j.medj.2022.11.002.
  PUBMED    36417917
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Med (N Y). 
            2022 Nov 15:S2666-6340(22)00456-1. doi: 10.1016/j.medj.2022.11.002."
COMMENT     Sequence Label: kiCAP-AAV-M.Fas.4
FEATURES             Location/Qualifiers
     source          1..9398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2782..2892
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     promoter        complement(2931..2949)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2932..2949)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2969..2986)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2975..3199
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3261..3279
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3298..3316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3335..3353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3372..3390
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3409..3427
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3446..3464
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3483..3501
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3520..3538
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             6255..6266
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(6607..6627)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6607..6625)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6646..6662)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6646..6662)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6659..6681)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6670..6686
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6694..6700,6701..6718,6719..6724))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6739..6760
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6877..6894)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7048..7636)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7128..7147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7807..8616)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8530..8549
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8617..8721)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8747..9202)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8884..8905)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9096..9115
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9329..9351
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9343..9360
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9344..9360
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9370..9389
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9370..9388
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 taccgggccc cccctcgatc gaggtcgaca cgcgtggagg ggtggagtcg tgacgtgaat
       61 tacgtcatag ggttagggag gtcctgtatt agaggtcacg tgagtgtttt gcgacatttt
      121 gcgacaccat gtggtcacgc tgggtattta agcccgagtg agcacgcagg gtctccattt
      181 tgaagcggga ggtttgaacg cgcagccgcc atgccggggt tttacgagat tgtgattaag
      241 gtccccagcg accttgacga gcatctgccc ggcatttctg acagctttgt gaactgggtg
      301 gccgagaagg aatgggagtt gccgccagat tctgacatgg atctgaatct gattgagcag
      361 gcacccctga ccgtggccga gaagctgcag cgcgactttc tgacggaatg gcgccgtgtg
      421 agtaaggccc cggaggctct tttctttgtg caatttgaga agggagagag ctacttccac
      481 atgcacgtgc tcgtggaaac caccggggtg aaatccatgg ttttgggacg tttcctgagt
      541 cagattcgcg aaaaactgat tcagagaatt taccgcggga tcgagccgac tttgccaaac
      601 tggttcgcgg tcacaaagac cagaaatggc gccggaggcg ggaacaaggt ggtggatgag
      661 tgctacatcc ccaattactt gctccccaaa acccagcctg agctccagtg ggcgtggact
      721 aatatggaac agtatttaag cgcctgtttg aatctcacgg agcgtaaacg gttggtggcg
      781 cagcatctga cgcacgtgtc gcagacgcag gagcagaaca aagagaatca gaatcccaat
      841 tctgatgcgc cggtgatcag atcaaaaact tcagccaggt acatggagct ggtcgggtgg
      901 ctcgtggaca aggggattac ctcggagaag cagtggatcc aggaggacca ggcctcatac
      961 atctccttca atgcggcctc caactcgcgg tcccaaatca aggctgcctt ggacaatgcg
     1021 ggaaagatta tgagcctgac taaaaccgcc cccgactacc tggtgggcca gcagcccgtg
     1081 gaggacattt ccagcaatcg gatttataaa attttggaac taaacgggta cgatccccaa
     1141 tatgcggctt ccgtctttct gggatgggcc acgaaaaagt tcggcaagag gaacaccatc
     1201 tggctgtttg ggcctgcaac taccgggaag accaacatcg cggaggccat agcccacact
     1261 gtgcccttct acgggtgcgt aaactggacc aatgagaact ttcccttcaa cgactgtgtc
     1321 gacaagatgg tgatctggtg ggaggagggg aagatgaccg ccaaggtcgt ggagtcggcc
     1381 aaagccattc tcggaggaag caaggtgcgc gtggaccaga aatgcaagtc ctcggcccag
     1441 atagacccga ctcccgtgat cgtcacctcc aacaccaaca tgtgcgccgt gattgacggg
     1501 aactcaacga ccttcgaaca ccagcagccg ttgcaagacc ggatgttcaa atttgaactc
     1561 acccgccgtc tggatcatga ctttgggaag gtcaccaagc aggaagtcaa agactttttc
     1621 cggtgggcaa aggatcacgt ggttgaggtg gagcatgaat tctacgtcaa aaagggtgga
     1681 gccaagaaaa gacccgcccc cagtgacgca gatataagtg agcccaaacg ggtgcgcgag
     1741 tcagttgcgc agccatcgac gtcagacgcg gaagcttcga tcaactacgc ggacaggtac
     1801 caaaacaaat gttctcgtca cgtgggcatg aatctgatgc tgtttccctg cagacaatgc
     1861 gagagactga atcagaattc aaatatctgc ttcactcacg gtgtcaaaga ctgtttagag
     1921 tgctttcccg tgtcagaatc tcaacccgtt tctgtcgtca aaaaggcgta tcagaaactg
     1981 tgctacattc atcacatcat gggaaaggtg ccagacgctt gcactgcttg cgacctggtc
     2041 aatgtggact tggatgactg tgtttctgaa caataaatga cttaaaccag gtattgctgc
     2101 cgatggttat cttccagatt ggctcgagga caaccttagt gaactagtgc caccatgagt
     2161 cggctggata aatctaaagt cataaacggc gctctggaat tactcaatga agtcggtatc
     2221 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac
     2281 tggcacgtga agaacaagcg ggccctgctc gatgccctgg ccatcgagat gctggacagg
     2341 catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac
     2401 gccaagtcat tccgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc
     2461 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag
     2521 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg
     2581 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc
     2641 accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg gcagggagcc
     2701 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag
     2761 tgcgaaagcg gcgggccggc cgacgccctt gacgattttg acttagacat gctcccagcc
     2821 gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac
     2881 cttgacatgc tccccgggta aatgcatgaa ttcgatctag agggccctat tctatagtgt
     2941 cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     3001 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3061 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3121 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3181 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gaatcaagct
     3241 atcaagtgcc acctgacgtc tccctatcag tgatagagaa gtcgacacgt ctcgagctcc
     3301 ctatcagtga tagagaaggt acgtctagaa cgtctcccta tcagtgatag agaagtcgac
     3361 acgtctcgag ctccctatca gtgatagaga aggtacgtct agaacgtctc cctatcagtg
     3421 atagagaagt cgacacgtct cgagctccct atcagtgata gagaaggtac gtctagaacg
     3481 tctccctatc agtgatagag aagtcgacac gtctcgagct ccctatcagt gatagagaag
     3541 gtacccccta tataagcaga gagatctgtt caaatttgaa ctgactaagc ggctcccgcc
     3601 agattttggc aagattacta agcaggaagt caaggacttt tttgcttggg caaaggtcaa
     3661 tcaggtgccg gtgactcacg agtttaaagt tcccagggaa ttggcgggaa ctaaaggggc
     3721 ggagaaatct ctaaaacgcc cactgggtga cgtcaccaat actagctata aaagtctgga
     3781 gaagcgggcc aggctctcat ttgttcccga gacgcctcgc agttcagacg tgactgttga
     3841 tcccgctcct ctgcgaccgc tagcttcgat caactacgcg gacaggtacc aaaacaaatg
     3901 ttctcgtcac gtgggcatga atctgatgct gtttccctgc agacaatgcg agagactgaa
     3961 tcagaattca aatatctgct tcactcacgg tgtcaaagac tgtttagagt gctttcccgt
     4021 gtcagaatct caacccgttt ctgtcgtcaa aaaggcgtat cagaaactgt gctacattca
     4081 tcacatcatg ggaaaggtgc cagacgcttg cactgcttgc gacctggtca atgtggactt
     4141 ggatgactgt gtttctgaac aataaatgac ttaaaccagg tatggctgcc gatggttatc
     4201 ttccagattg gctcgaggac aaccttagtg aaggaattcg cgagtggtgg gctttgaaac
     4261 ctggagcccc tcaacccaag gcaaatcaac aacatcaaga caacgctcga ggtcttgtgc
     4321 ttccgggtta caaatacctt ggacccggca acggactcga caagggggag ccggtcaacg
     4381 cagcagacgc ggcggccctc gagcacgaca aggcctacga ccagcagctc aaggccggag
     4441 acaacccgta cctcaagtac aaccacgccg acgccgagtt ccaggagcgg ctcaaagaag
     4501 atacgtcttt tgggggcaac ctcgggcgag cagtcttcca ggccaaaaag aggcttcttg
     4561 aacctcttgg tctggttgag gaagcggcta agacggctcc tggaaagaag aggcctgtag
     4621 agcagtctcc tcaggaaccg gactcctccg cgggtattgg caaatcgggt gcacagcccg
     4681 ctaaaaagag actcaatttc ggtcagactg gcgacacaga gtcagtccca gaccctcaac
     4741 caatcggaga acctcccgca gccccctcag gtgtgggatc tcttacaatg gcttcaggtg
     4801 gtggcgcacc agtggcagac aataacgaag gtgccgatgg agtgggtagt tcctcgggaa
     4861 attggcattg cgattcccaa tggctggggg acagagtcat caccaccagc acccgaacct
     4921 gggccctgcc cacctacaac aatcacctct acaagcaaat ctccaacagc acatctggag
     4981 gatcttcaaa tgacaacgcc tacttcggct acagcacccc ctgggggtat tttgacttca
     5041 acagattcca ctgccacttc tcaccacgtg actggcagcg actcatcaac aacaactggg
     5101 gattccggcc taagcgactc aacttcaagc tcttcaacat tcaggtcaaa gaggttacgg
     5161 acaacaatgg agtcaagacc atcgccaata accttaccag cacggtccag gtcttcacgg
     5221 actcagacta tcagctcccg tacgtgctcg ggtcggctca cgagggctgc ctcccgccgt
     5281 tcccagcgga cgttttcatg attcctcagt acgggtatct gacgcttaat gatggaagcc
     5341 aggccgtggg tcgttcgtcc ttttactgcc tggaatattt cccgtcgcaa atgctaagaa
     5401 cgggtaacaa cttccagttc agctacgagt ttgagaacgt acctttccat agcagctacg
     5461 ctcacagcca aagcctggac cgactaatga atccactcat cgaccaatac ttgtactatc
     5521 tctcaaagac tattaacggt tctggacaga atcaacaaac gctaaaattc agtgtggccg
     5581 gacccagcaa catggctgtc cagggaagaa actacatacc tggacccagc taccgacaac
     5641 aacgtgtctc aaccactgtg actcaaaaca acaacagcga atttgcttgg cctggagctt
     5701 cttcttgggc tctcaatgga cgtaatagct tgatgaatcc tggacctgct atggccagcc
     5761 acaaagaagg agaggaccgt ttctttcctt tgtctggatc tttaattttt ggcaaacaag
     5821 gaactggaag agacaacgtg gatgcggaca aagtcatgat aaccaacgaa gaagaaatta
     5881 aaactactaa cccggtagca acggagtcct atggacaagt ggccacaaac caccagagtg
     5941 cccaagttgt tagtgattat actgtggcac aggcgcagac cggctgggtt caaaaccaag
     6001 gaatacttcc gggtatggtt tggcaggaca gagatgtgta cctgcaagga cccatttggg
     6061 ccaaaattcc tcacacggac ggcaactttc acccttctcc gctgatggga gggtttggaa
     6121 tgaagcaccc gcctcctcag atcctcatca aaaacacacc tgtacctgcg gatcctccaa
     6181 cggccttcaa caaggacaag ctgaactctt tcatcaccca gtattctact ggccaagtca
     6241 gcgtggagat cgagtgggag ctgcagaagg aaaacagcaa gcgctggaac ccggagatcc
     6301 agtacacttc caactattac aagtctaata atgttgaatt tgctgttaat actgaaggtg
     6361 tatatagtga accccgcccc attggcacca gatacctgac tcgtaatctg taattgcttg
     6421 ttaatcaata aaccgtttaa ttcgtttcag ttgaactttg gtctctgcgt atttctttct
     6481 tatctagttt ccatggctac gtagataagt agcatggcgg gttaatcatt aactacagat
     6541 atccatcaca ctggcggccg ctcgactaga gcggccgcca ccgcggtgga gctccagctt
     6601 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     6661 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6721 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6781 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6841 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6901 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6961 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7021 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7081 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7141 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7201 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7261 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7321 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7381 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7441 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7501 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7561 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7621 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7681 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7741 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7801 tgacagttag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
     7861 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
     7921 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
     7981 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     8041 gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac
     8101 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
     8161 tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
     8221 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     8281 aggatattct tctaatacct ggaatgctgt ttttccgggg atcgcagtgg tgagtaacca
     8341 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     8401 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     8461 cagaaacaac tctggcgcat cgggcttccc atacaagcga tagattgtcg cacctgattg
     8521 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     8581 tcgcggcctc gacgtttccc gttgaatatg gctcatactc ttcctttttc aatattattg
     8641 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8701 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     8761 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     8821 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     8881 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     8941 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     9001 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     9061 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     9121 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     9181 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     9241 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     9301 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     9361 gagcgcgcgt aatacgactc actatagggc gaattggg
//
