LOCUS       sequence-389723-        9589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' 
            HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains 
            FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Truong DJ, Armbrust N, Geilenkeuser J, Lederer EM, Santl TH, Beyer 
            M, Ittermann S, Steinmassl E, Dyka M, Raffl G, Phlairaharn T, 
            Greisle T, Zivanic M, Grosch M, Drukker M, Westmeyer GG
  TITLE     Intron-encoded cistronic transcripts for minimally invasive 
            monitoring of coding and non-coding RNAs.
  JOURNAL   Nat Cell Biol. 2022 Nov;24(11):1666-1676. doi: 
            10.1038/s41556-022-00998-6. Epub 2022 Nov 7.
  PUBMED    36344775
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-022-00998-6"; journalName: "Nat Cell Biol"; date: 
            "2022-11"; volume: "24"; issue: "11"; pages: "1666-1676"
COMMENT     Sequence Label: pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR
FEATURES             Location/Qualifiers
     source          1..9589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1064..1097
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1168..1201
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     polyA_signal    1800..1921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1837..1856)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1891..1910
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1953..1972
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1971..2026
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    complement(2133..2166)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2237..2270)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(2319..2375)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3510..3566)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     4153..4172
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(4199..5381)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4208..5150
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4231..4251)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    join(5457..6030,6031..6033,6034..6042)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5624..5641)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5851..5870
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(7401..7421)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7401..7419)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7440..7456)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7440..7456)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7453..7475)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7464..7480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7488..7494,7495..7512,7513..7518))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7533..7554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7671..7688)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7842..8430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7922..7941)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8601..9392,9393..9461))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9224..9243
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9462..9566)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggaggtctc
      661 tgtaagtatc ctttttacag cacaacttaa tgagacagat agaaactggt cttgtagaaa
      721 cagagtagac acttgctttt gacacaactg tgtttacttg caatccccca aaacagacag
      781 atagtcaggc cagccggtta ggctgccacc gaaggttggt agacggtgct gcctgcgacc
      841 aaccccagga ggactgggtt accaaagctg attctccacg gttggaaagc ctcccagaac
      901 cgtctcggaa gaggagtccc tgccaacaat ggagatgaag cccgtgtcag atcgcgaaag
      961 cgccacttcg ccgaggagtg caatctgtga ggccccagga ggactgggta aacagtcgac
     1021 ccgggcggcc gcattttatc gaattgcaac ctcttcgagg gacataactt cgtataatgt
     1081 atgctatacg aagttatggg tcgatgctga gatctggact agagggtcga tggtgatgct
     1141 tggatcaaaa taggaagaat cccaatgata acttcgtata aagtatccta tacgaagtta
     1201 tactagaatt gcgtaccgcc accatgtcgc caaggccacc tcccctgtga gctagactgg
     1261 acagccaatg acgggtaaga gagtgacatt tctcactaac ctaagacagg agggccgtca
     1321 aagctactgc ctaatccaat gacgggtaat agtgacaaga aatgtatcac tccaacctaa
     1381 gacaggcgca gcctccgagg gatgtgtctt ttgtttttta taattaaaaa gggtgacacg
     1441 tgaggccacc tcccctgtga gctagactgg acagccaatg acgggtaaga gagtgacatt
     1501 tctcactaac ctaagacagg agggccgtca aagctactgc ctaatccaat gacgggtaat
     1561 agtgacaaga aatgtatcac tccaacctaa gacaggcgca gcctccgagg gatgtgtctt
     1621 ttgtttttta taattaaaaa gggtgaagcc gacacacaaa aaaccaacac acagatctct
     1681 agagctctgg atcttttatt gcccgggcta ccacatttgt agaggtttta cttgctttaa
     1741 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     1801 acttgtttat tgcagcttat aatggttaca aatatagcaa tagcatcaca aatttcacaa
     1861 atatagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     1921 atcatgtctg tatactttaa atgatttgcc ctcccatatg tccttccgag tgagagacac
     1981 aaaaaattcc aacacactat tgcaatgaaa atacatttcc tttattagcc agaagtcaga
     2041 tgccagcaca acaaccagca cgttgcccag gagctgtagg aaaaagaaga aggcatgaac
     2101 atagttagca gagttacgta gcgatcgcta gtataacttc gtatagcata cattatacga
     2161 agttatgggt cgatggtgac aatagcgaaa aagaaagaac aatcaagggt ccccaaactc
     2221 acctaggtcc tttgatataa cttcgtatag gatactttat acgaagttat ggcatctcct
     2281 tcggaagttc ctattctatt tgaagtatag gaacttccag gtccaggatt ttcctcgacg
     2341 tcccctgcct gcttgagcag tgaaaagtta gttgccccgg atccgttggc ttcgcccatc
     2401 tctctagcga aggttctggc gaggtcacag atggtgggga tgctgccagg ggttgtcaca
     2461 tgggtctgca ccatgccgct tgtcagttgc agcagggcat ctctgcatcc ggctgggctc
     2521 tgatcgtagt ccaggatgaa cacatgcatg ggccgcagtc tttttgccag cacgtcgaga
     2581 gcccaggcga acacgttata caggtcgccg ttaggggcca gcagttcagg ggctctgaac
     2641 agtgtgaaca gggtgtcgcc gatgtgaggt ctgggtccag cgttagactg aggctcagcg
     2701 ccttgtggag gaactgctgt gccagagagc tgtccccagt cctctctcca ggaaccgccg
     2761 ccttgaagat agcgcacggt gttagccagc aggccgtaca ctcttctgat ggcggccagc
     2821 atagccagat ccagtctctc gccaggcctc tgtctcttag ccagtctgtc gatgtgtctg
     2881 tcctctggca gggctccaag cacgatgttt gtgccgggca gtgtaggagg aatcagagcc
     2941 acgaaagcca gcacggcctg aggtgtcatg ctgcccatca gataccgagc ggcagggtaa
     3001 cacagcaggg cgaagatggg gtgtctgtcg aagatcaggg tcagagcagg tggtggagcg
     3061 tgagaagatc cagcctcgcc gccaatatgt ggagccagca cagcgtctgt cacggcgtaa
     3121 ggcatgccca ttgtgatctg ggcagatgtc atgaccacag cagcgtcgcc tgcgctaatt
     3181 tcgccctggt ccagtctgtg ctgtgtggtg tagatgttgg cgattgtctc agaggctccc
     3241 aggacctgcc agtaggtcat aggctcgggc acgtacacga tgtcatctct gctgcccaga
     3301 gccaccagca gctgtgttgt tgtggtcttg cccatgccat gagggccgtc gatatacact
     3361 ctcagcagtg tgggcatctt ctgttccagc cgcacttctg tagcctcttg ctgccttctt
     3421 ggcctgaggg ctgttcttct gttgctgtgg cctctgcttc tagcggcttg atcgaaggcg
     3481 ctggcgtgct ggtgacaagg ataagaagca ggaccggggt tctcttccac gtcgccagcc
     3541 tgtttcagca ggctgaagtt tgtagcgccg gatccggcgc caggctttct ggtcatgcac
     3601 caggttctag ggccctcagg cacttccacg tcggcggtca cggtgaagcc cagtctctcg
     3661 tagaagggca ggtttctggg ggcgcttgtt tccaggaagg cgggcacgcc agccctttca
     3721 gcagcttcca ccccaggcag caccacagca gatcccagtc ccttgccctg gtggtcaggt
     3781 gacacgccca cggtggccag aaaccaggca ggctcttttg gtctgtgggg ggccagcagg
     3841 ccttccatct gctgctgggc agccagtcta gagccgctca gctcggccat tctaggtccg
     3901 atctcggcga acacagcgcc ggcttccaca gactcagggg ttgtccacac agccacagcg
     3961 gcgccatcat cggccaccca cactttgccg atgtccaggc ccactctggt cagaaacagt
     4021 tcctgcagct cggtcactct ctcgatgtgc cggtcggggt ccacggtgtg tcttgtggca
     4081 gggtaatcgg cgaaggcagc ggccagtgtc cgcacagctc ttggcacatc gtccctggtg
     4141 gccagccgca ctgtgggctt gtactcggtc atggtggcgc gccttttagg ggtagttttc
     4201 acgacacctg aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc
     4261 aagatccaaa ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc
     4321 taacttcagt ctccacccac tcagtgtggg gaaactccat cgcataaaac ccctcccccc
     4381 aacctaaaga cgacgtactc caaaagctcg agaactaatc gaggtgcctg gacggcgccc
     4441 ggtactccgt ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg
     4501 tgggtgactc acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctccctgcag
     4561 cagggccggg aagcggccat ctttccgctc acgcaactgg tgccgaccgg gccagccttg
     4621 ccgcccaggg cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag
     4681 cttgagacta cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac
     4741 atgtgcgctg ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag
     4801 tgtgcagatc ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag
     4861 gtcatcaaaa attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt
     4921 gccaccagat tcgcacgcgg cggccccagc gcccaggcca ggcctcaact caagcacgag
     4981 gcgaaggggc tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct
     5041 cgggatcaag aatcacgtac tgcagccagg ggcgtggaag taattcaagg cacgcaaggg
     5101 ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg
     5161 gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct
     5221 cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg
     5281 cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt ctcggggact
     5341 gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc caattcgaag ttcctattct
     5401 atttgaagta taggaacttc aaatacaagg gcttactgtg cggactggga cgcccccccc
     5461 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     5521 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     5581 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     5641 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     5701 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     5761 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     5821 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     5881 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     5941 tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg
     6001 tggttttcct ttgaaaaaca cgatgataat atggccacca ccatggtctt tacactggaa
     6061 gatttcgtcg gcgactggcg gcagacagct ggctacaatc tggaccaggt gctggaacaa
     6121 ggcggcgtgt cctctctgtt tcaaaacctg ggagtgtctg tgacccctat ccagagaatc
     6181 gtgctgagcg gcgagaacgg cctgaagatc gacatccatg tgatcatccc ttacgagggc
     6241 ctgtccggcg atcagatggg ccagatcgag aagattttca aggtggtgta ccccgtggac
     6301 gaccaccact tcaaagtgat cctgcactac ggcaccctgg tcatcgatgg cgtgacccct
     6361 aacatgatcg actacttcgg cagaccctac gagggaatcg ccgtgttcga cggcaagaaa
     6421 atcaccgtga ccggcacact gtggaacggc aacaagatca tcgacgagcg gctgatcaac
     6481 cccgatggca gcctgctgtt cagagtgacc atcaacggcg tgacaggatg gcggctgtgc
     6541 gaaagaatcc tggcctaatg tacaaacggg gagctaaaca ctccaggcca ataggccatc
     6601 ctgttttttt cccttttttt ttttcttttt tttttttttt tttttttttt tttttttttc
     6661 tccttttttt ttcctctttt tttccttttc tttcctttgg tggctccatc ttagccctag
     6721 tcacggctag ctgtgaaagg tccgtgagcc gcttgactgc agagagtgct gatactggcc
     6781 tctctgcaga tcaagtgata tcactatcga taccgcgggg atcctctaga gtagaccacc
     6841 tcccctgcga gctaagctgg acagccaatg acgactaaga gagtgacatt tttcactaag
     6901 ctaagacagg agggccgtca gagctactgc ctaatccaaa gacggctaaa agtgataaaa
     6961 atgtatcact ccaagttaag acaggcgcag cttccgaggg atttgcctgg ccggattgac
     7021 tgacgtctta accactgcat atcgataccg cggggatcct ctagagtaga ccacctcccc
     7081 tgcgagctaa gctggacagc caatgacgac taagagagtg acatttttca ctaagctaag
     7141 acaggagggc cgtcagagct actgcctaat ccaaagacgg ctaaaagtga taaaaatgta
     7201 tcactccaag ttaagacagg cgcagcttcc gagggatttg cgtttaaacg cggccgctct
     7261 atactcgacg attgactatc tgacaaatca tagagaacat agagaacctc ctcgagtcgc
     7321 ctgcttttct gcctggtact aacttctctc ccctctcctc ttttcttttt ctgcagggag
     7381 accgtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     7441 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7501 gggagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7561 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7621 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7681 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7741 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7801 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7861 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7921 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7981 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8041 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8101 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8161 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8221 aggtatgtag gaggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8281 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8341 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8401 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8461 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8521 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8581 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8641 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8701 gagggcttac catctggccc cagtgctgca atgataccgc gagatccacg ctcaccggct
     8761 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8821 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8881 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8941 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9001 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9061 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9121 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9181 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9241 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9301 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9361 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9421 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9481 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9541 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
