LOCUS       sequence-389724-        9113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Backbone for INSPECT expression. Encodes for intronic 
            IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK 
            cassette. Clone homology arms via Esp3I..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9113)
  AUTHORS   Truong DJ, Armbrust N, Geilenkeuser J, Lederer EM, Santl TH, Beyer 
            M, Ittermann S, Steinmassl E, Dyka M, Raffl G, Phlairaharn T, 
            Greisle T, Zivanic M, Grosch M, Drukker M, Westmeyer GG
  TITLE     Intron-encoded cistronic transcripts for minimally invasive 
            monitoring of coding and non-coding RNAs.
  JOURNAL   Nat Cell Biol. 2022 Nov;24(11):1666-1676. doi: 
            10.1038/s41556-022-00998-6. Epub 2022 Nov 7.
  PUBMED    36344775
REFERENCE   2  (bases 1 to 9113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-022-00998-6"; journalName: "Nat Cell Biol"; date: 
            "2022-11"; volume: "24"; issue: "11"; pages: "1666-1676"
COMMENT     Sequence Label: pINSPECT_SigP:NLuc
FEATURES             Location/Qualifiers
     source          1..9113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    763..796
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    867..900
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     polyA_signal    1499..1620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1536..1555)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1590..1609
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1652..1671
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1670..1725
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    complement(1832..1865)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(1936..1969)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(2018..2074)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3209..3265)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     3852..3871
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3898..5080)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3907..4849
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(3930..3950)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    join(5156..5729,5730..5732,5733..5741)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5323..5340)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5550..5569
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6925..6945)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6925..6943)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6964..6980)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6964..6980)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6977..6999)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6988..7004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7012..7018,7019..7036,7037..7042))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7057..7078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7195..7212)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7366..7954)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7446..7465)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8125..8916,8917..8985))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8748..8767
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8986..9090)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgt
      661 ctcggtaagt atccttttta cagcacaact taatgagaca gatagaaact ggtcttgtag
      721 aaacagagta gattttatcg aattgcaacc tcttcgaggg acataacttc gtataatgta
      781 tgctatacga agttatgggt cgatgctgag atctggacta gagggtcgat ggtgatgctt
      841 ggatcaaaat aggaagaatc ccaatgataa cttcgtataa agtatcctat acgaagttat
      901 actagaattg cgtaccgcca ccatgtcgcc aaggccacct cccctgtgag ctagactgga
      961 cagccaatga cgggtaagag agtgacattt ctcactaacc taagacagga gggccgtcaa
     1021 agctactgcc taatccaatg acgggtaata gtgacaagaa atgtatcact ccaacctaag
     1081 acaggcgcag cctccgaggg atgtgtcttt tgttttttat aattaaaaag ggtgacacgt
     1141 gaggccacct cccctgtgag ctagactgga cagccaatga cgggtaagag agtgacattt
     1201 ctcactaacc taagacagga gggccgtcaa agctactgcc taatccaatg acgggtaata
     1261 gtgacaagaa atgtatcact ccaacctaag acaggcgcag cctccgaggg atgtgtcttt
     1321 tgttttttat aattaaaaag ggtgaagccg acacacaaaa aaccaacaca cagatctcta
     1381 gagctctgga tcttttattg cccgggctac cacatttgta gaggttttac ttgctttaaa
     1441 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     1501 cttgtttatt gcagcttata atggttacaa atatagcaat agcatcacaa atttcacaaa
     1561 tatagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     1621 tcatgtctgt atactttaaa tgatttgccc tcccatatgt ccttccgagt gagagacaca
     1681 aaaaattcca acacactatt gcaatgaaaa tacatttcct ttattagcca gaagtcagat
     1741 gccagcacaa caaccagcac gttgcccagg agctgtagga aaaagaagaa ggcatgaaca
     1801 tagttagcag agttacgtag cgatcgctag tataacttcg tatagcatac attatacgaa
     1861 gttatgggtc gatggtgaca atagcgaaaa agaaagaaca atcaagggtc cccaaactca
     1921 cctaggtcct ttgatataac ttcgtatagg atactttata cgaagttatg gcatctcctt
     1981 cggaagttcc tattctattt gaagtatagg aacttccagg tccaggattt tcctcgacgt
     2041 cccctgcctg cttgagcagt gaaaagttag ttgccccgga tccgttggct tcgcccatct
     2101 ctctagcgaa ggttctggcg aggtcacaga tggtggggat gctgccaggg gttgtcacat
     2161 gggtctgcac catgccgctt gtcagttgca gcagggcatc tctgcatccg gctgggctct
     2221 gatcgtagtc caggatgaac acatgcatgg gccgcagtct ttttgccagc acgtcgagag
     2281 cccaggcgaa cacgttatac aggtcgccgt taggggccag cagttcaggg gctctgaaca
     2341 gtgtgaacag ggtgtcgccg atgtgaggtc tgggtccagc gttagactga ggctcagcgc
     2401 cttgtggagg aactgctgtg ccagagagct gtccccagtc ctctctccag gaaccgccgc
     2461 cttgaagata gcgcacggtg ttagccagca ggccgtacac tcttctgatg gcggccagca
     2521 tagccagatc cagtctctcg ccaggcctct gtctcttagc cagtctgtcg atgtgtctgt
     2581 cctctggcag ggctccaagc acgatgtttg tgccgggcag tgtaggagga atcagagcca
     2641 cgaaagccag cacggcctga ggtgtcatgc tgcccatcag ataccgagcg gcagggtaac
     2701 acagcagggc gaagatgggg tgtctgtcga agatcagggt cagagcaggt ggtggagcgt
     2761 gagaagatcc agcctcgccg ccaatatgtg gagccagcac agcgtctgtc acggcgtaag
     2821 gcatgcccat tgtgatctgg gcagatgtca tgaccacagc agcgtcgcct gcgctaattt
     2881 cgccctggtc cagtctgtgc tgtgtggtgt agatgttggc gattgtctca gaggctccca
     2941 ggacctgcca gtaggtcata ggctcgggca cgtacacgat gtcatctctg ctgcccagag
     3001 ccaccagcag ctgtgttgtt gtggtcttgc ccatgccatg agggccgtcg atatacactc
     3061 tcagcagtgt gggcatcttc tgttccagcc gcacttctgt agcctcttgc tgccttcttg
     3121 gcctgagggc tgttcttctg ttgctgtggc ctctgcttct agcggcttga tcgaaggcgc
     3181 tggcgtgctg gtgacaagga taagaagcag gaccggggtt ctcttccacg tcgccagcct
     3241 gtttcagcag gctgaagttt gtagcgccgg atccggcgcc aggctttctg gtcatgcacc
     3301 aggttctagg gccctcaggc acttccacgt cggcggtcac ggtgaagccc agtctctcgt
     3361 agaagggcag gtttctgggg gcgcttgttt ccaggaaggc gggcacgcca gccctttcag
     3421 cagcttccac cccaggcagc accacagcag atcccagtcc cttgccctgg tggtcaggtg
     3481 acacgcccac ggtggccaga aaccaggcag gctcttttgg tctgtggggg gccagcaggc
     3541 cttccatctg ctgctgggca gccagtctag agccgctcag ctcggccatt ctaggtccga
     3601 tctcggcgaa cacagcgccg gcttccacag actcaggggt tgtccacaca gccacagcgg
     3661 cgccatcatc ggccacccac actttgccga tgtccaggcc cactctggtc agaaacagtt
     3721 cctgcagctc ggtcactctc tcgatgtgcc ggtcggggtc cacggtgtgt cttgtggcag
     3781 ggtaatcggc gaaggcagcg gccagtgtcc gcacagctct tggcacatcg tccctggtgg
     3841 ccagccgcac tgtgggcttg tactcggtca tggtggcgcg ccttttaggg gtagttttca
     3901 cgacacctga aatggaagaa aaaaactttg aaccactgtc tgaggcttga gaatgaacca
     3961 agatccaaac tcaaaaaggg caaattccaa ggagaattac atcaagtgcc aagctggcct
     4021 aacttcagtc tccacccact cagtgtgggg aaactccatc gcataaaacc cctcccccca
     4081 acctaaagac gacgtactcc aaaagctcga gaactaatcg aggtgcctgg acggcgcccg
     4141 gtactccgtg gagtcacatg aagcgacggc tgaggacgga aaggcccttt tcctttgtgt
     4201 gggtgactca cccgcccgct ctcccgagcg ccgcgtcctc cattttgagc tccctgcagc
     4261 agggccggga agcggccatc tttccgctca cgcaactggt gccgaccggg ccagccttgc
     4321 cgcccagggc ggggcgatac acggcggcgc gaggccaggc accagagcag gccggccagc
     4381 ttgagactac ccccgtccga ttctcggtgg ccgcgctcgc aggccccgcc tcgccgaaca
     4441 tgtgcgctgg gacgcacggg ccccgtcgcc gcccgcggcc ccaaaaaccg aaataccagt
     4501 gtgcagatct tggcccgcat ttacaagact atcttgccag aaaaaaagcg tcgcagcagg
     4561 tcatcaaaaa ttttaaatgg ctagagactt atcgaaagca gcgagacagg cgcgaaggtg
     4621 ccaccagatt cgcacgcggc ggccccagcg cccaggccag gcctcaactc aagcacgagg
     4681 cgaaggggct ccttaagcgc aaggcctcga actctcccac ccacttccaa cccgaagctc
     4741 gggatcaaga atcacgtact gcagccaggg gcgtggaagt aattcaaggc acgcaagggc
     4801 cataacccgt aaagaggcca ggcccgcggg aaccacacac ggcacttacc tgtgttctgg
     4861 cggcaaaccc gttgcgaaaa agaacgttca cggcgactac tgcacttata tacggttctc
     4921 ccccaccctc gggaaaaagg cggagccagt acacgacatc actttcccag tttaccccgc
     4981 gccaccttct ctaggcaccg gttcaattgc cgacccctcc ccccaacttc tcggggactg
     5041 tgggcgatgt gcgctctgcc cactgacggg caccggagcc aattcgaagt tcctattcta
     5101 tttgaagtat aggaacttca aatacaaggg cttactgtgc ggactgggac gcccccccct
     5161 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     5221 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     5281 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     5341 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     5401 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     5461 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     5521 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     5581 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     5641 gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt
     5701 ggttttcctt tgaaaaacac gatgataata tggccaccac catggtggtc aaggtgctgt
     5761 tcgccctgat ctgtattgcc gtggccgagg ccaagcctac cgtgttcacc ctggaagatt
     5821 tcgtcggcga ttggagacag accgccggat acaacctgga ccaggtgttg gagcaaggcg
     5881 gcgtgtccag cctgtttcag aacctgggag tgtccgtgac acccatccag agaatcgtgc
     5941 tgagcggcga gaacggcctg aagatcgaca tccatgtgat catcccttac gagggcctgt
     6001 ccggcgatca gatgggccag atcgagaaga ttttcaaggt ggtgtacccc gtggacgacc
     6061 accacttcaa agtgatcctg cactacggca ccctggtcat cgatggcgtg acccctaaca
     6121 tgatcgacta cttcggcaga ccctacgagg gaatcgccgt gttcgacggc aagaaaatca
     6181 ccgtgaccgg cacactgtgg aacggcaaca agatcatcga cgagcggctg atcaaccccg
     6241 atggcagcct gctgttcaga gtgaccatca acggcgtgac aggatggcgg ctgtgcgaaa
     6301 gaatcctggc ctaatgtaca gatatcacta tcgataccgc ggggatcctc tagagtagac
     6361 cacctcccct gcgagctaag ctggacagcc aatgacgact aagagagtga catttttcac
     6421 taagctaaga caggagggcc gtcagagcta ctgcctaatc caaagacggc taaaagtgat
     6481 aaaaatgtat cactccaagt taagacaggc gcagcttccg agggatttgc ctggccggat
     6541 tgactgacgt cttaaccact gcatatcgat accgcgggga tcctctagag tagaccacct
     6601 cccctgcgag ctaagctgga cagccaatga cgactaagag agtgacattt ttcactaagc
     6661 taagacagga gggccgtcag agctactgcc taatccaaag acggctaaaa gtgataaaaa
     6721 tgtatcactc caagttaaga caggcgcagc ttccgaggga tttgcgttta aacgcggccg
     6781 ctctatactc gacgattgac tatctgacaa atcatagaga acatagagaa cctcctcgag
     6841 tcgcctgctt ttctgcctgg tactaacttc tctcccctct cctcttttct ttttctgcag
     6901 ggagacggta cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     6961 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7021 agccgggagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7081 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7141 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7201 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7261 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7321 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7381 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7441 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7501 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7561 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7621 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7681 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7741 agcgaggtat gtaggaggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7801 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7861 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7921 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7981 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8041 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8101 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8161 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8221 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8281 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8341 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8401 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8461 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8521 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8581 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8641 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8701 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8761 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8821 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8881 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8941 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9001 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9061 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
