LOCUS       sequence-389739-        3144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses LuxR in forward direction under the control of BBa_J23102 
            constitutive promoter, ColE1 origin of replication, Ampicillin 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Sarkar K, Bonnerjee D, Srivastava R, Bagh S
  TITLE     A single layer artificial neural network type architecture with 
            molecular engineered bacteria for reversible and irreversible 
            computing.
  JOURNAL   Chem Sci. 2021 Nov 9;12(48):15821-15832. doi: 10.1039/d1sc01505b. 
            eCollection 2021 Dec 15.
  PUBMED    35024106
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/d1sc01505b"; journalName: "Chem Sci"; date: "2021-11-9- 9";
            volume: "12"; issue: "48"; pages: "15821-15832"
COMMENT     Sequence Label: pJA2LUXR(F)
FEATURES             Location/Qualifiers
     source          1..3144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     79..97
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        118..152
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     RBS             162..173
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      990..1076
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1349..1937)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1429..1448)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2025..2119)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2149..2940,2941..3009))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2772..2791
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3010..3114)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctactgggac gaagacgaac agtcgacaac gaagacgcca acatcaagat ctttattatc
       61 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca cctcgagttg
      121 acagctagct cagtcctagg tactgtgcta gcgaattcat taaagaggag aaaggtacca
      181 tgaaaaacat aaatgccgac gacacataca gaataattaa taaaattaaa gcttgtagaa
      241 gcaataatga tattaatcaa tgcttatctg atatgactaa aatggtacat tgtgaatatt
      301 atttactcgc gatcatttat cctcattcta tggttaaatc tgatatttca atcctagata
      361 attaccctaa aaaatggagg caatattatg atgacgctaa tttaataaaa tatgatccta
      421 tagtagatta ttctaactcc aatcattcac caattaattg gaatatattt gaaaacaatg
      481 ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa aacatcaggt cttatcactg
      541 ggtttagttt ccctattcat acggctaaca atggcttcgg aatgcttagt tttgcacatt
      601 cagaaaaaga caactatata gatagtttat ttttacatgc gtgtatgaac ataccattaa
      661 ttgttccttc tctagttgat aattatcgaa aaataaatat agcaaataat aaatcaaaca
      721 acgatttaac caaaagagaa aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt
      781 gggatatttc aaaaatatta ggttgcagtg agcgtactgt cactttccat ttaaccaatg
      841 cgcaaatgaa actcaataca acaaaccgct gccaaagtat ttctaaagca attttaacag
      901 gagcaattga ttgcccatac tttaaaaatt aataacactg atagtgctag tgtagatcac
      961 tactagagcc aggcatcatc tagaggcatc aaataaaacg aaaggctcag tcgaaagact
     1021 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     1081 cgccctagac ctaggcgatg acggaaaaag agatcgtctt aatgtataga ttttcgtgga
     1141 ttacgtcgcc agtcaggatc ctggcagaag ctattcattc tgcagttccg tgctccaaaa
     1201 caaccgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1261 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1321 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1381 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1441 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1501 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1561 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1621 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1681 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1741 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     1801 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1861 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1921 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1981 acgaaaactc acgttaaggg attttggtca tgactagtgc ttggattctc accaataaaa
     2041 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     2101 ggatctatca acaggagtcc aagcgagctc gtaaacttgg tctgacagtt accaatgctt
     2161 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2221 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2281 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2341 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2401 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2461 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2521 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2581 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2641 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2701 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2761 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2821 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2881 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2941 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3001 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3061 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3121 tccccgaaaa gtgccacctg acgt
//
