LOCUS       sequence-389740-        9398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rep/Cap plasmid for the production of PAL1A, an AAV capsid with CNS 
            tropism in macaques..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Stanton AC, Lagerborg KA, Tellez L, Krunnfusz A, King EM, Ye S, 
            Solomon IH, Tabebordbar M, Sabeti PC
  TITLE     Systemic administration of novel engineered AAV capsids facilitates 
            enhanced transgene expression in the macaque CNS.
  JOURNAL   Med (N Y). 2022 Nov 15:S2666-6340(22)00456-1. doi: 
            10.1016/j.medj.2022.11.002.
  PUBMED    36417917
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Med (N Y). 
            2022 Nov 15:S2666-6340(22)00456-1. doi: 10.1016/j.medj.2022.11.002."
COMMENT     Sequence Label: kiCAP-AAV-PAL1A
FEATURES             Location/Qualifiers
     source          1..9398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2770..2880
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     promoter        complement(2919..2937)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2920..2937)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2957..2974)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2963..3187
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3249..3267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3286..3304
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3323..3341
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3360..3378
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3397..3415
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3434..3452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3471..3489
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3508..3526
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             6243..6254
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(6595..6615)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6595..6613)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6634..6650)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6634..6650)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6647..6669)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6658..6674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6682..6688,6689..6706,6707..6712))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6727..6748
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6865..6882)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7036..7624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7116..7135)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7795..8604)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8518..8537
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8605..8709)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8735..9190)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8872..8893)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9084..9103
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9317..9339
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9331..9348
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9332..9348
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9358..9377
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9358..9376
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cctcgatcga ggtcgacacg cgtggagggg tggagtcgtg acgtgaatta cgtcataggg
       61 ttagggaggt cctgtattag aggtcacgtg agtgttttgc gacattttgc gacaccatgt
      121 ggtcacgctg ggtatttaag cccgagtgag cacgcagggt ctccattttg aagcgggagg
      181 tttgaacgcg cagccgccat gccggggttt tacgagattg tgattaaggt ccccagcgac
      241 cttgacgagc atctgcccgg catttctgac agctttgtga actgggtggc cgagaaggaa
      301 tgggagttgc cgccagattc tgacatggat ctgaatctga ttgagcaggc acccctgacc
      361 gtggccgaga agctgcagcg cgactttctg acggaatggc gccgtgtgag taaggccccg
      421 gaggctcttt tctttgtgca atttgagaag ggagagagct acttccacat gcacgtgctc
      481 gtggaaacca ccggggtgaa atccatggtt ttgggacgtt tcctgagtca gattcgcgaa
      541 aaactgattc agagaattta ccgcgggatc gagccgactt tgccaaactg gttcgcggtc
      601 acaaagacca gaaatggcgc cggaggcggg aacaaggtgg tggatgagtg ctacatcccc
      661 aattacttgc tccccaaaac ccagcctgag ctccagtggg cgtggactaa tatggaacag
      721 tatttaagcg cctgtttgaa tctcacggag cgtaaacggt tggtggcgca gcatctgacg
      781 cacgtgtcgc agacgcagga gcagaacaaa gagaatcaga atcccaattc tgatgcgccg
      841 gtgatcagat caaaaacttc agccaggtac atggagctgg tcgggtggct cgtggacaag
      901 gggattacct cggagaagca gtggatccag gaggaccagg cctcatacat ctccttcaat
      961 gcggcctcca actcgcggtc ccaaatcaag gctgccttgg acaatgcggg aaagattatg
     1021 agcctgacta aaaccgcccc cgactacctg gtgggccagc agcccgtgga ggacatttcc
     1081 agcaatcgga tttataaaat tttggaacta aacgggtacg atccccaata tgcggcttcc
     1141 gtctttctgg gatgggccac gaaaaagttc ggcaagagga acaccatctg gctgtttggg
     1201 cctgcaacta ccgggaagac caacatcgcg gaggccatag cccacactgt gcccttctac
     1261 gggtgcgtaa actggaccaa tgagaacttt cccttcaacg actgtgtcga caagatggtg
     1321 atctggtggg aggaggggaa gatgaccgcc aaggtcgtgg agtcggccaa agccattctc
     1381 ggaggaagca aggtgcgcgt ggaccagaaa tgcaagtcct cggcccagat agacccgact
     1441 cccgtgatcg tcacctccaa caccaacatg tgcgccgtga ttgacgggaa ctcaacgacc
     1501 ttcgaacacc agcagccgtt gcaagaccgg atgttcaaat ttgaactcac ccgccgtctg
     1561 gatcatgact ttgggaaggt caccaagcag gaagtcaaag actttttccg gtgggcaaag
     1621 gatcacgtgg ttgaggtgga gcatgaattc tacgtcaaaa agggtggagc caagaaaaga
     1681 cccgccccca gtgacgcaga tataagtgag cccaaacggg tgcgcgagtc agttgcgcag
     1741 ccatcgacgt cagacgcgga agcttcgatc aactacgcgg acaggtacca aaacaaatgt
     1801 tctcgtcacg tgggcatgaa tctgatgctg tttccctgca gacaatgcga gagactgaat
     1861 cagaattcaa atatctgctt cactcacggt gtcaaagact gtttagagtg ctttcccgtg
     1921 tcagaatctc aacccgtttc tgtcgtcaaa aaggcgtatc agaaactgtg ctacattcat
     1981 cacatcatgg gaaaggtgcc agacgcttgc actgcttgcg acctggtcaa tgtggacttg
     2041 gatgactgtg tttctgaaca ataaatgact taaaccaggt attgctgccg atggttatct
     2101 tccagattgg ctcgaggaca accttagtga actagtgcca ccatgagtcg gctggataaa
     2161 tctaaagtca taaacggcgc tctggaatta ctcaatgaag tcggtatcga aggcctgacg
     2221 acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag
     2281 aacaagcggg ccctgctcga tgccctggcc atcgagatgc tggacaggca tcatacccac
     2341 ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcattc
     2401 cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca
     2461 gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc
     2521 ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg
     2581 gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg
     2641 cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc
     2701 cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc
     2761 gggccggccg acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac
     2821 gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc
     2881 cccgggtaaa tgcatgaatt cgatctagag ggccctattc tatagtgtca cctaaatgct
     2941 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3001 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3061 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3121 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     3181 tctatggctt ctgaggcgga aagaaccagc tggggctcga atcaagctat caagtgccac
     3241 ctgacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct atcagtgata
     3301 gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac gtctcgagct
     3361 ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat agagaagtcg
     3421 acacgtctcg agctccctat cagtgataga gaaggtacgt ctagaacgtc tccctatcag
     3481 tgatagagaa gtcgacacgt ctcgagctcc ctatcagtga tagagaaggt accccctata
     3541 taagcagaga gatctgttca aatttgaact gactaagcgg ctcccgccag attttggcaa
     3601 gattactaag caggaagtca aggacttttt tgcttgggca aaggtcaatc aggtgccggt
     3661 gactcacgag tttaaagttc ccagggaatt ggcgggaact aaaggggcgg agaaatctct
     3721 aaaacgccca ctgggtgacg tcaccaatac tagctataaa agtctggaga agcgggccag
     3781 gctctcattt gttcccgaga cgcctcgcag ttcagacgtg actgttgatc ccgctcctct
     3841 gcgaccgcta gcttcgatca actacgcgga caggtaccaa aacaaatgtt ctcgtcacgt
     3901 gggcatgaat ctgatgctgt ttccctgcag acaatgcgag agactgaatc agaattcaaa
     3961 tatctgcttc actcacggtg tcaaagactg tttagagtgc tttcccgtgt cagaatctca
     4021 acccgtttct gtcgtcaaaa aggcgtatca gaaactgtgc tacattcatc acatcatggg
     4081 aaaggtgcca gacgcttgca ctgcttgcga cctggtcaat gtggacttgg atgactgtgt
     4141 ttctgaacaa taaatgactt aaaccaggta tggctgccga tggttatctt ccagattggc
     4201 tcgaggacaa ccttagtgaa ggaattcgcg agtggtgggc tttgaaacct ggagcccctc
     4261 aacccaaggc aaatcaacaa catcaagaca acgctcgagg tcttgtgctt ccgggttaca
     4321 aataccttgg acccggcaac ggactcgaca agggggagcc ggtcaacgca gcagacgcgg
     4381 cggccctcga gcacgacaag gcctacgacc agcagctcaa ggccggagac aacccgtacc
     4441 tcaagtacaa ccacgccgac gccgagttcc aggagcggct caaagaagat acgtcttttg
     4501 ggggcaacct cgggcgagca gtcttccagg ccaaaaagag gcttcttgaa cctcttggtc
     4561 tggttgagga agcggctaag acggctcctg gaaagaagag gcctgtagag cagtctcctc
     4621 aggaaccgga ctcctccgcg ggtattggca aatcgggtgc acagcccgct aaaaagagac
     4681 tcaatttcgg tcagactggc gacacagagt cagtcccaga ccctcaacca atcggagaac
     4741 ctcccgcagc cccctcaggt gtgggatctc ttacaatggc ttcaggtggt ggcgcaccag
     4801 tggcagacaa taacgaaggt gccgatggag tgggtagttc ctcgggaaat tggcattgcg
     4861 attcccaatg gctgggggac agagtcatca ccaccagcac ccgaacctgg gccctgccca
     4921 cctacaacaa tcacctctac aagcaaatct ccaacagcac atctggagga tcttcaaatg
     4981 acaacgccta cttcggctac agcaccccct gggggtattt tgacttcaac agattccact
     5041 gccacttctc accacgtgac tggcagcgac tcatcaacaa caactgggga ttccggccta
     5101 agcgactcaa cttcaagctc ttcaacattc aggtcaaaga ggttacggac aacaatggag
     5161 tcaagaccat cgccaataac cttaccagca cggtccaggt cttcacggac tcagactatc
     5221 agctcccgta cgtgctcggg tcggctcacg agggctgcct cccgccgttc ccagcggacg
     5281 ttttcatgat tcctcagtac gggtatctga cgcttaatga tggaagccag gccgtgggtc
     5341 gttcgtcctt ttactgcctg gaatatttcc cgtcgcaaat gctaagaacg ggtaacaact
     5401 tccagttcag ctacgagttt gagaacgtac ctttccatag cagctacgct cacagccaaa
     5461 gcctggaccg actaatgaat ccactcatcg accaatactt gtactatctc tcaaagacta
     5521 ttaacggttc tggacagaat caacaaacgc taaaattcag tgtggccgga cccagcaaca
     5581 tggctgtcca gggaagaaac tacatacctg gacccagcta ccgacaacaa cgtgtctcaa
     5641 ccactgtgac tcaaaacaac aacagcgaat ttgcttggcc tggagcttct tcttgggctc
     5701 tcaatggacg taatagcttg atgaatcctg gacctgctat ggccagccac aaagaaggag
     5761 aggaccgttt ctttcctttg tctggatctt taatttttgg caaacaagga actggaagag
     5821 acaacgtgga tgcggacaaa gtcatgataa ccaacgaaga agaaattaaa actactaacc
     5881 cggtagcaac ggagtcctat ggacaagtgg ccacaaacca ccagagtgcc caacccacac
     5941 aaggcacagt ccgtgcacag gcgcagaccg gctgggttca aaaccaagga atacttccgg
     6001 gtatggtttg gcaggacaga gatgtgtacc tgcaaggacc catttgggcc aaaattcctc
     6061 acacggacgg caactttcac ccttctccgc tgatgggagg gtttggaatg aagcacccgc
     6121 ctcctcagat cctcatcaaa aacacacctg tacctgcgga tcctccaacg gccttcaaca
     6181 aggacaagct gaactctttc atcacccagt attctactgg ccaagtcagc gtggagatcg
     6241 agtgggagct gcagaaggaa aacagcaagc gctggaaccc ggagatccag tacacttcca
     6301 actattacaa gtctaataat gttgaatttg ctgttaatac tgaaggtgta tatagtgaac
     6361 cccgccccat tggcaccaga tacctgactc gtaatctgta attgcttgtt aatcaataaa
     6421 ccgtttaatt cgtttcagtt gaactttggt ctctgcgtat ttctttctta tctagtttcc
     6481 atggctacgt agataagtag catggcgggt taatcattaa ctacagatat ccatcacact
     6541 ggcggccgct cgactagagc ggccgccacc gcggtggagc tccagctttt gttcccttta
     6601 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6661 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6721 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6841 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6901 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6961 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7021 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7081 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7141 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7201 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7261 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7321 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7381 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7441 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7501 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7561 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7621 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7681 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7741 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttagaa
     7801 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     7861 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     7921 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     7981 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     8041 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
     8101 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     8161 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     8221 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     8281 taatacctgg aatgctgttt ttccggggat cgcagtggtg agtaaccatg catcatcagg
     8341 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     8401 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     8461 tggcgcatcg ggcttcccat acaagcgata gattgtcgca cctgattgcc cgacattatc
     8521 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga
     8581 cgtttcccgt tgaatatggc tcatactctt cctttttcaa tattattgaa gcatttatca
     8641 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8701 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     8761 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     8821 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     8881 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     8941 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     9001 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     9061 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     9121 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     9181 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     9241 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     9301 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
     9361 tacgactcac tatagggcga attgggtacc gggccccc
//
