LOCUS       sequence-389747-        9398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rep/Cap plasmid for the production of PAL1B, an AAV capsid with CNS 
            tropism in macaques..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Stanton AC, Lagerborg KA, Tellez L, Krunnfusz A, King EM, Ye S, 
            Solomon IH, Tabebordbar M, Sabeti PC
  TITLE     Systemic administration of novel engineered AAV capsids facilitates 
            enhanced transgene expression in the macaque CNS.
  JOURNAL   Med (N Y). 2022 Nov 15:S2666-6340(22)00456-1. doi: 
            10.1016/j.medj.2022.11.002.
  PUBMED    36417917
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Med (N Y). 
            2022 Nov 15:S2666-6340(22)00456-1. doi: 10.1016/j.medj.2022.11.002."
COMMENT     Sequence Label: kiCAP-AAV-PAL1B
FEATURES             Location/Qualifiers
     source          1..9398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2767..2877
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     promoter        complement(2916..2934)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2917..2934)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2954..2971)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2960..3184
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3246..3264
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3283..3301
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3320..3338
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3357..3375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3394..3412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3431..3449
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3468..3486
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3505..3523
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             6240..6251
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(6592..6612)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6592..6610)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6631..6647)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6631..6647)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6644..6666)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6655..6671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6679..6685,6686..6703,6704..6709))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6724..6745
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6862..6879)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7033..7621)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7113..7132)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7792..8601)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8515..8534
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8602..8706)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8732..9187)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8869..8890)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9081..9100
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9314..9336
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9328..9345
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9329..9345
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9355..9374
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9355..9373
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgatcgaggt cgacacgcgt ggaggggtgg agtcgtgacg tgaattacgt catagggtta
       61 gggaggtcct gtattagagg tcacgtgagt gttttgcgac attttgcgac accatgtggt
      121 cacgctgggt atttaagccc gagtgagcac gcagggtctc cattttgaag cgggaggttt
      181 gaacgcgcag ccgccatgcc ggggttttac gagattgtga ttaaggtccc cagcgacctt
      241 gacgagcatc tgcccggcat ttctgacagc tttgtgaact gggtggccga gaaggaatgg
      301 gagttgccgc cagattctga catggatctg aatctgattg agcaggcacc cctgaccgtg
      361 gccgagaagc tgcagcgcga ctttctgacg gaatggcgcc gtgtgagtaa ggccccggag
      421 gctcttttct ttgtgcaatt tgagaaggga gagagctact tccacatgca cgtgctcgtg
      481 gaaaccaccg gggtgaaatc catggttttg ggacgtttcc tgagtcagat tcgcgaaaaa
      541 ctgattcaga gaatttaccg cgggatcgag ccgactttgc caaactggtt cgcggtcaca
      601 aagaccagaa atggcgccgg aggcgggaac aaggtggtgg atgagtgcta catccccaat
      661 tacttgctcc ccaaaaccca gcctgagctc cagtgggcgt ggactaatat ggaacagtat
      721 ttaagcgcct gtttgaatct cacggagcgt aaacggttgg tggcgcagca tctgacgcac
      781 gtgtcgcaga cgcaggagca gaacaaagag aatcagaatc ccaattctga tgcgccggtg
      841 atcagatcaa aaacttcagc caggtacatg gagctggtcg ggtggctcgt ggacaagggg
      901 attacctcgg agaagcagtg gatccaggag gaccaggcct catacatctc cttcaatgcg
      961 gcctccaact cgcggtccca aatcaaggct gccttggaca atgcgggaaa gattatgagc
     1021 ctgactaaaa ccgcccccga ctacctggtg ggccagcagc ccgtggagga catttccagc
     1081 aatcggattt ataaaatttt ggaactaaac gggtacgatc cccaatatgc ggcttccgtc
     1141 tttctgggat gggccacgaa aaagttcggc aagaggaaca ccatctggct gtttgggcct
     1201 gcaactaccg ggaagaccaa catcgcggag gccatagccc acactgtgcc cttctacggg
     1261 tgcgtaaact ggaccaatga gaactttccc ttcaacgact gtgtcgacaa gatggtgatc
     1321 tggtgggagg aggggaagat gaccgccaag gtcgtggagt cggccaaagc cattctcgga
     1381 ggaagcaagg tgcgcgtgga ccagaaatgc aagtcctcgg cccagataga cccgactccc
     1441 gtgatcgtca cctccaacac caacatgtgc gccgtgattg acgggaactc aacgaccttc
     1501 gaacaccagc agccgttgca agaccggatg ttcaaatttg aactcacccg ccgtctggat
     1561 catgactttg ggaaggtcac caagcaggaa gtcaaagact ttttccggtg ggcaaaggat
     1621 cacgtggttg aggtggagca tgaattctac gtcaaaaagg gtggagccaa gaaaagaccc
     1681 gcccccagtg acgcagatat aagtgagccc aaacgggtgc gcgagtcagt tgcgcagcca
     1741 tcgacgtcag acgcggaagc ttcgatcaac tacgcggaca ggtaccaaaa caaatgttct
     1801 cgtcacgtgg gcatgaatct gatgctgttt ccctgcagac aatgcgagag actgaatcag
     1861 aattcaaata tctgcttcac tcacggtgtc aaagactgtt tagagtgctt tcccgtgtca
     1921 gaatctcaac ccgtttctgt cgtcaaaaag gcgtatcaga aactgtgcta cattcatcac
     1981 atcatgggaa aggtgccaga cgcttgcact gcttgcgacc tggtcaatgt ggacttggat
     2041 gactgtgttt ctgaacaata aatgacttaa accaggtatt gctgccgatg gttatcttcc
     2101 agattggctc gaggacaacc ttagtgaact agtgccacca tgagtcggct ggataaatct
     2161 aaagtcataa acggcgctct ggaattactc aatgaagtcg gtatcgaagg cctgacgaca
     2221 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
     2281 aagcgggccc tgctcgatgc cctggccatc gagatgctgg acaggcatca tacccacttc
     2341 tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcattccgc
     2401 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
     2461 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
     2521 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
     2581 gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
     2641 ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt
     2701 ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg
     2761 ccggccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac
     2821 tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc
     2881 gggtaaatgc atgaattcga tctagagggc cctattctat agtgtcacct aaatgctaga
     2941 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3001 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3061 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3121 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     3181 atggcttctg aggcggaaag aaccagctgg ggctcgaatc aagctatcaa gtgccacctg
     3241 acgtctccct atcagtgata gagaagtcga cacgtctcga gctccctatc agtgatagag
     3301 aaggtacgtc tagaacgtct ccctatcagt gatagagaag tcgacacgtc tcgagctccc
     3361 tatcagtgat agagaaggta cgtctagaac gtctccctat cagtgataga gaagtcgaca
     3421 cgtctcgagc tccctatcag tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga
     3481 tagagaagtc gacacgtctc gagctcccta tcagtgatag agaaggtacc ccctatataa
     3541 gcagagagat ctgttcaaat ttgaactgac taagcggctc ccgccagatt ttggcaagat
     3601 tactaagcag gaagtcaagg acttttttgc ttgggcaaag gtcaatcagg tgccggtgac
     3661 tcacgagttt aaagttccca gggaattggc gggaactaaa ggggcggaga aatctctaaa
     3721 acgcccactg ggtgacgtca ccaatactag ctataaaagt ctggagaagc gggccaggct
     3781 ctcatttgtt cccgagacgc ctcgcagttc agacgtgact gttgatcccg ctcctctgcg
     3841 accgctagct tcgatcaact acgcggacag gtaccaaaac aaatgttctc gtcacgtggg
     3901 catgaatctg atgctgtttc cctgcagaca atgcgagaga ctgaatcaga attcaaatat
     3961 ctgcttcact cacggtgtca aagactgttt agagtgcttt cccgtgtcag aatctcaacc
     4021 cgtttctgtc gtcaaaaagg cgtatcagaa actgtgctac attcatcaca tcatgggaaa
     4081 ggtgccagac gcttgcactg cttgcgacct ggtcaatgtg gacttggatg actgtgtttc
     4141 tgaacaataa atgacttaaa ccaggtatgg ctgccgatgg ttatcttcca gattggctcg
     4201 aggacaacct tagtgaagga attcgcgagt ggtgggcttt gaaacctgga gcccctcaac
     4261 ccaaggcaaa tcaacaacat caagacaacg ctcgaggtct tgtgcttccg ggttacaaat
     4321 accttggacc cggcaacgga ctcgacaagg gggagccggt caacgcagca gacgcggcgg
     4381 ccctcgagca cgacaaggcc tacgaccagc agctcaaggc cggagacaac ccgtacctca
     4441 agtacaacca cgccgacgcc gagttccagg agcggctcaa agaagatacg tcttttgggg
     4501 gcaacctcgg gcgagcagtc ttccaggcca aaaagaggct tcttgaacct cttggtctgg
     4561 ttgaggaagc ggctaagacg gctcctggaa agaagaggcc tgtagagcag tctcctcagg
     4621 aaccggactc ctccgcgggt attggcaaat cgggtgcaca gcccgctaaa aagagactca
     4681 atttcggtca gactggcgac acagagtcag tcccagaccc tcaaccaatc ggagaacctc
     4741 ccgcagcccc ctcaggtgtg ggatctctta caatggcttc aggtggtggc gcaccagtgg
     4801 cagacaataa cgaaggtgcc gatggagtgg gtagttcctc gggaaattgg cattgcgatt
     4861 cccaatggct gggggacaga gtcatcacca ccagcacccg aacctgggcc ctgcccacct
     4921 acaacaatca cctctacaag caaatctcca acagcacatc tggaggatct tcaaatgaca
     4981 acgcctactt cggctacagc accccctggg ggtattttga cttcaacaga ttccactgcc
     5041 acttctcacc acgtgactgg cagcgactca tcaacaacaa ctggggattc cggcctaagc
     5101 gactcaactt caagctcttc aacattcagg tcaaagaggt tacggacaac aatggagtca
     5161 agaccatcgc caataacctt accagcacgg tccaggtctt cacggactca gactatcagc
     5221 tcccgtacgt gctcgggtcg gctcacgagg gctgcctccc gccgttccca gcggacgttt
     5281 tcatgattcc tcagtacggg tatctgacgc ttaatgatgg aagccaggcc gtgggtcgtt
     5341 cgtcctttta ctgcctggaa tatttcccgt cgcaaatgct aagaacgggt aacaacttcc
     5401 agttcagcta cgagtttgag aacgtacctt tccatagcag ctacgctcac agccaaagcc
     5461 tggaccgact aatgaatcca ctcatcgacc aatacttgta ctatctctca aagactatta
     5521 acggttctgg acagaatcaa caaacgctaa aattcagtgt ggccggaccc agcaacatgg
     5581 ctgtccaggg aagaaactac atacctggac ccagctaccg acaacaacgt gtctcaacca
     5641 ctgtgactca aaacaacaac agcgaatttg cttggcctgg agcttcttct tgggctctca
     5701 atggacgtaa tagcttgatg aatcctggac ctgctatggc cagccacaaa gaaggagagg
     5761 accgtttctt tcctttgtct ggatctttaa tttttggcaa acaaggaact ggaagagaca
     5821 acgtggatgc ggacaaagtc atgataacca acgaagaaga aattaaaact actaacccgg
     5881 tagcaacgga gtcctatgga caagtggcca caaaccacca gagtgcccaa ccttctcagg
     5941 ggacgcttcg ggcacaggcg cagaccggct gggttcaaaa ccaaggaata cttccgggta
     6001 tggtttggca ggacagagat gtgtacctgc aaggacccat ttgggccaaa attcctcaca
     6061 cggacggcaa ctttcaccct tctccgctga tgggagggtt tggaatgaag cacccgcctc
     6121 ctcagatcct catcaaaaac acacctgtac ctgcggatcc tccaacggcc ttcaacaagg
     6181 acaagctgaa ctctttcatc acccagtatt ctactggcca agtcagcgtg gagatcgagt
     6241 gggagctgca gaaggaaaac agcaagcgct ggaacccgga gatccagtac acttccaact
     6301 attacaagtc taataatgtt gaatttgctg ttaatactga aggtgtatat agtgaacccc
     6361 gccccattgg caccagatac ctgactcgta atctgtaatt gcttgttaat caataaaccg
     6421 tttaattcgt ttcagttgaa ctttggtctc tgcgtatttc tttcttatct agtttccatg
     6481 gctacgtaga taagtagcat ggcgggttaa tcattaacta cagatatcca tcacactggc
     6541 ggccgctcga ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg
     6601 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     6661 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6721 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6781 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6841 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6901 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6961 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7021 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7081 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7141 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7201 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7261 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7321 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7381 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7441 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7501 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7561 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7621 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7681 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7741 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttagaaaaa
     7801 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt
     7861 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc
     7921 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt
     7981 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg
     8041 tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg
     8101 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc
     8161 gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg
     8221 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa
     8281 tacctggaat gctgtttttc cggggatcgc agtggtgagt aaccatgcat catcaggagt
     8341 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac
     8401 catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg
     8461 cgcatcgggc ttcccataca agcgatagat tgtcgcacct gattgcccga cattatcgcg
     8521 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgacgt
     8581 ttcccgttga atatggctca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8641 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8701 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     8761 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     8821 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     8881 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     8941 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     9001 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     9061 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     9121 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     9181 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     9241 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     9301 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
     9361 gactcactat agggcgaatt gggtaccggg ccccccct
//
