LOCUS       sequence-389753-       16122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sortable and selectable SpCas9 fused to exodeoxyribonuclease I 
            (sbcB) from E. coli. For the creation of longer deletions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16122)
  TITLE     Schmierer unpublished plasmids
REFERENCE   2  (bases 1 to 16122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: lenti-Cas9-Exo-Blast-BFP
FEATURES             Location/Qualifiers
     source          1..16122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(19..607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(99..118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(778..1569,1570..1638))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1401..1420
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1639..1743)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1818..1837)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2009..2388
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2390..2588
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2543..2563
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2606..2786
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2833..2958
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3451..3684
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3869..3913
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4211..4328
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4458..5636
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4689..5627
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5584..5604
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5652..5699
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5700..9800
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5700..9800
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9807..9827
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11355..11375
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11391..11444
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             11445..11840
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             11862..12560
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     misc_feature    12585..13173
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12638..12658)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13056..13067)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13176..13192)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13177..13193)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             13698..13878
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(13904..13921)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13910..14134
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      14180..14608
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14267..14286)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14477..14498
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(14617..14637)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14622..14951
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      14802..14937
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     14864..14883
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        14999..15046
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             15065..15439
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    15569..15690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15606..15625)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15660..15679
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(15739..15755)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15739..15755)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15752..15774)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15763..15779
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15787..15793,15794..15811,15812..15817))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15832..15853
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15970..15987)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
       61 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      121 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      181 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      241 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      301 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      361 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      421 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
      481 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      541 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      601 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      661 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
      721 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
      781 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      841 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      901 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
      961 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1021 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     1081 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     1141 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     1201 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     1261 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     1321 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     1381 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     1441 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     1501 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     1561 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     1621 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     1681 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1741 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     1801 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     1861 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     1921 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     1981 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     2041 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     2101 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     2161 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     2221 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     2281 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     2341 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     2401 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     2461 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     2521 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     2581 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     2641 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     2701 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     2761 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     2821 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     2881 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     2941 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     3001 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     3061 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     3121 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     3181 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     3241 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     3301 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     3361 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     3421 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     3481 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     3541 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     3601 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     3661 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     3721 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     3781 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     3841 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     3901 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     3961 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     4021 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     4081 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     4141 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
     4201 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     4261 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     4321 aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt agctagctgc
     4381 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
     4441 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     4501 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     4561 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     4621 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     4681 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     4741 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     4801 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     4861 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     4921 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     4981 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     5041 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     5101 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     5161 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     5221 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     5281 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     5341 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     5401 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     5461 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     5521 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     5581 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacgta
     5641 cggccaccat gaaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg
     5701 acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg gccgtgatca
     5761 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca
     5821 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca
     5881 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc
     5941 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg
     6001 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca
     6061 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac
     6121 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga
     6181 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg
     6241 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca
     6301 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc
     6361 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggaaacctga
     6421 ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg
     6481 ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga
     6541 tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc
     6601 tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga
     6661 tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc
     6721 agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct
     6781 acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa
     6841 agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc
     6901 agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca
     6961 ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga
     7021 agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat
     7081 tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg
     7141 tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc
     7201 tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata
     7261 acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg
     7321 gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga
     7381 agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg
     7441 gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca
     7501 aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga
     7561 ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc
     7621 tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc
     7681 tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt
     7741 tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc
     7801 tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg
     7861 agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga
     7921 aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg
     7981 aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga
     8041 agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg
     8101 aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata
     8161 tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gaccatatcg
     8221 tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca
     8281 agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact
     8341 actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca
     8401 aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg
     8461 tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta
     8521 agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc
     8581 tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc
     8641 accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc
     8701 ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga
     8761 tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca
     8821 tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc
     8881 tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca
     8941 ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga
     9001 caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca
     9061 gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt
     9121 ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag
     9181 agctactggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc
     9241 tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact
     9301 ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga
     9361 agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact
     9421 atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc
     9481 acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc
     9541 tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca
     9601 tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg
     9661 ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg
     9721 tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc
     9781 tgtctcagct gggaggcgac agcggaccta agaaaaagag gaaggtggcg gccgcttctg
     9841 gaggatctag cggaggatcc tctggcagcg agacaccagg aacaagcgag tcagcaacac
     9901 cagagagcag tggcggcagc agcggcggca gcagcaacga cgggaagcag cagtcaacat
     9961 ttctttttca cgactacgag accttcggca cccaccccgc cctggataga cccgctcagt
    10021 tcgccgccat cagaaccgac agcgagttca acgtgatcgg cgagcctgaa gtgttctact
    10081 gcaagcccgc tgacgattac ctgcctcagc ctggcgctgt gctgatcacc ggcatcaccc
    10141 ctcaggaggc cagagccaag ggcgagaacg aggccgcctt cgctgcccgg atccacagcc
    10201 tgttcaccgt gccaaagacc tgtatcctgg gatacaacaa cgtgcggttc gacgacgagg
    10261 tgaccagaaa catcttctac cggaacttct acgaccccta cgcctggagc tggcaacacg
    10321 ataacagcag atgggacctg ctggacgtga tgagagcatg ctacgccctg cggcctgagg
    10381 gcatcaactg gcccgagaac gatgacggcc tgccatcttt tagactggaa catctgacca
    10441 aggccaacgg cattgagcat tctaatgccc acgacgccat ggccgatgtg tacgccacaa
    10501 tcgccatggc caaactggtg aaaacaagac agccgcggct gtttgactac ctgtttacac
    10561 acagaaacaa gcacaagctg atggccctga tcgacgtgcc ccagatgaaa cctctggtcc
    10621 atgtgtccgg catgttcggc gcctggcggg gcaacaccag ctgggtcgcc cctctggcct
    10681 ggcaccctga gaacagaaac gctgtgatca tggtggacct ggccggcgac atcagccctc
    10741 tgctggaact ggacagcgac accctgcgag aaagactgta caccgccaag acagacctgg
    10801 gcgataacgc cgctgttcct gtgaagctgg tccacatcaa caagtgcccc gtgctggctc
    10861 aggccaatac cctgcgtcca gaagatgccg accggctggg aattaaccgg cagcactgcc
    10921 tggacaacct gaagatcctg agagagaatc ctcaagtgcg ggaaaaagtg gtggcaattt
    10981 tcgccgaggc cgaacctttc acacctagcg ataatgtgga cgcccagctg tacaacggct
    11041 tcttcagcga cgctgataga gccgctatga aaatcgtgct ggaaacggaa cccagaaacc
    11101 tgcctgctct cgatatcaca ttcgtggata agagaatcga gaagctgctg tttaactaca
    11161 gagccagaaa tttccccgga acactggatt acgccgaaca gcaaagatgg ctggaacacc
    11221 ggagacaggt gttcacccct gaattcctgc agggctacgc tgatgagctg cagatgctgg
    11281 tgcagcagta cgccgatgac aaggaaaagg tggccctgct taaagccctg tggcagtacg
    11341 ccgaggaaat cgtgcctaaa aagaagcgta aagtaggatc cggcagtgga gagggcagag
    11401 gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaatggcc aagcctttgt
    11461 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct
    11521 ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg
    11581 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg
    11641 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca
    11701 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag
    11761 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct
    11821 ctggttatgt gtgggagggc gctagcggca gtggaagtgg cagcgagctg attaaggaga
    11881 acatgcacat gaagctctac atggagggca ccgtggacaa ccatcacttc aagtgcacat
    11941 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg
    12001 gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga
    12061 ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct
    12121 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
    12181 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat
    12241 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc gaaacgctgt
    12301 accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga
    12361 gccatctgat cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca
    12421 agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg
    12481 agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac
    12541 tggggcacaa gcttaattaa gaattcgata tcaagcttat cggtaatcaa cctctggatt
    12601 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
    12661 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
    12721 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
    12781 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
    12841 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
    12901 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
    12961 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
    13021 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
    13081 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
    13141 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
    13201 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
    13261 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
    13321 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
    13381 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
    13441 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
    13501 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
    13561 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
    13621 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
    13681 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    13741 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    13801 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    13861 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
    13921 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
    13981 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
    14041 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
    14101 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
    14161 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    14221 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    14281 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    14341 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    14401 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    14461 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    14521 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    14581 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
    14641 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
    14701 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    14761 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
    14821 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    14881 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    14941 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
    15001 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
    15061 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
    15121 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
    15181 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
    15241 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
    15301 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
    15361 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
    15421 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
    15481 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
    15541 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
    15601 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    15661 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
    15721 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    15781 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    15841 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    15901 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    15961 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    16021 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    16081 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aa
//
