LOCUS       sequence-389788-        9953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral plasmid for expressing cytosol localized TurboID 
            with EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9953)
  AUTHORS   Yan T, Julio AR, Villanueva M, Jones AE, Ball AB, Boatner LM, Turmon
            AC, Nguyen KB, Yen SL, Desai HS, Divakaruni AS, Backus KM
  TITLE     Proximity-labeling chemoproteomics defines the subcellular 
            cysteinome and inflammation-responsive mitochondrial redoxome.
  JOURNAL   Cell Chem Biol. 2023 Jul 20;30(7):811-827.e7. doi: 
            10.1016/j.chembiol.2023.06.008. Epub 2023 Jul 6.
  PUBMED    37419112
REFERENCE   2  (bases 1 to 9953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2023.06.008"; journalName: "Cell Chem Biol"; 
            date: "2023-07-20- 20"; volume: "30"; issue: "7"; pages: 
            "811-827.e7"
COMMENT     Sequence Label: FUGW-Cytosol-TurboID
FEATURES             Location/Qualifiers
     source          1..9953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(656..1447,1448..1516))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1279..1298
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1517..1621)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1696..1715)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1887..2266
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2268..2466
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2421..2441
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2484..2664
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2711..2836
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3329..3562
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3747..3791
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4089..4206
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4271..5481
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5318..5337
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5529..5538
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5535..5537,5538..5540,5541..6254)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5535..5537,5538..5540,5541..6251)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5580..5601)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5841..5860)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6188..6209
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6295..6883
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6348..6368)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6766..6777)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6886..6902)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6887..6903)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7408..7588
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7614..7631)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7620..7844
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7890..8318
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7977..7996)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8187..8208
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8327..8347)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8332..8661
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8512..8647
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8574..8593
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8709..8756
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8775..9149
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9279..9400
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9316..9335)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9370..9389
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9449..9465)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9449..9465)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9462..9484)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9473..9489
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9497..9503,9504..9521,9522..9527))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9542..9563
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9680..9697)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9931..9950)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
       61 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      121 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
      181 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
      241 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
      301 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
      361 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
      421 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
      481 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
      541 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
      601 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
      661 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
      721 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
      781 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
      841 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
      901 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
      961 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1021 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1081 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1141 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1201 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1261 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     1321 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1381 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     1441 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     1501 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     1561 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     1621 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     1681 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     1741 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     1801 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     1861 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     1921 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1981 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     2041 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     2101 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2161 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2221 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2281 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2341 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2401 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2461 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     2521 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     2581 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     2641 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     2701 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     2761 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     2821 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     2881 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     2941 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     3001 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     3061 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     3121 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     3181 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     3241 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     3301 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     3361 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     3421 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     3481 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     3541 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     3601 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     3661 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     3721 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     3781 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     3841 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     3901 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     3961 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     4021 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     4081 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     4141 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     4201 aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa cccgtgtcgg
     4261 ctccagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg
     4321 gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg
     4381 ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag
     4441 gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga
     4501 acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt
     4561 gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg
     4621 ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg
     4681 ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg
     4741 gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt
     4801 ggggggagcg cacaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc
     4861 gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct
     4921 tgaggccttc gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct
     4981 ggggaccctg acgtgaagtt tgtcactgac tggagaactc gggtttgtcg tctggttgcg
     5041 ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgcct
     5101 cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg
     5161 gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct
     5221 ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt
     5281 ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg
     5341 cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca
     5401 tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg
     5461 tttttggctt ttttgttaga cgaagcttgg gctgcaggtc gactctagag gatccccggg
     5521 taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     5581 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     5641 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     5701 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     5761 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     5821 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     5881 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     5941 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     6001 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     6061 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     6121 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     6181 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     6241 agctgtacaa gtaaagcggc cgcgactcta gaattcgata tcaagcttat cgataatcaa
     6301 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6361 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6421 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6481 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6541 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6601 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6661 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6721 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6781 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6841 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     6901 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     6961 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     7021 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7081 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     7141 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     7201 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     7261 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     7321 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     7381 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     7441 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7501 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7561 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     7621 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     7681 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     7741 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     7801 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     7861 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     7921 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7981 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8041 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8101 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8161 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8221 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     8281 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     8341 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8401 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8461 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     8521 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8581 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     8641 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     8701 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     8761 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     8821 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     8881 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     8941 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     9001 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     9061 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     9121 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     9181 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     9241 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     9301 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9361 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     9421 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     9481 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     9541 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     9601 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     9661 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     9721 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     9781 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     9841 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     9901 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cct
//
