LOCUS       sequence-389821-       10189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  35S promoter-driven expression of the positive-sense TSWV S RNA 
            segment encoding sgRNA:GFP fusion in place of viral NSs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Liu Q, Zhao C, Sun K, Deng Y, Li Z
  TITLE     Engineered biocontainable RNA virus vectors for non-transgenic 
            genome editing across crop species and genotypes.
  JOURNAL   Mol Plant. 2023 Mar 6;16(3):616-631. doi: 
            10.1016/j.molp.2023.02.003. Epub 2023 Feb 7.
  PUBMED    36751129
REFERENCE   2  (bases 1 to 10189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molp.2023.02.003"; journalName: "Mol Plant"; date: 
            "2023-03-6- 6"; volume: "16"; issue: "3"; pages: "616-631"
COMMENT     Sequence Label: pS-sg:GFP
FEATURES             Location/Qualifiers
     source          1..10189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        423..768
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     711..731
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             complement(join(2241..2954,2955..2957,2958..2960))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVPLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2286..2307)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2635..2654
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2894..2915
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_RNA        complement(2967..3042)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      3258..3510
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3558..3582
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      3710..5052
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(4673..4720)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     5093..5111
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5149..5171)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5271..5290
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5311..5592
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             5593..6267
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(6370..6825)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6507..6528)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6719..6738
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(6941..8089)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8388..9182)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9391..10022
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    10102..10126
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
       61 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
      121 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
      181 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
      241 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag
      361 cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca
      421 attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct
      481 atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat
      541 tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga
      601 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
      661 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
      721 caagaccctt cctctatata aggaagttca tttcatttgg agaggagagc aattgtgtca
      781 attttattca aaccttaaca ctcagtctta caaatcatca cattaaaacc ctaagaaacg
      841 actgcggaat acagagttgt actttcgcac cttgaattac atacggtcaa agcatataac
      901 aacttctacg atcatcatgt ctaaggttaa gctcactaag gaaaacattg ttgctttgtt
      961 gacacaaggc aaagatcttg aatttgaaga agatcagaat ctggtagcat ttaacttcaa
     1021 gactttttgt ctggaaaacc ttgaccagat caagaagatg agcattattt catgtctgac
     1081 attcctgaag aatcgtcaga gtataatgaa ggttattaag caaagtgatt ttacttttgg
     1141 caaaatcact ataaagaaaa cttcagacag gattggagcc actgacatga ccttcagaag
     1201 gcttgatagc ttgatcaggg tcaggcttgt cgaggaaact gggaattctg agaatctcaa
     1261 tactatcaaa tctaagattg cttctcaccc tctgattcaa gcctatggat tacctcttga
     1321 tgatgcaaag tctgtgaggc ttgccataat gctgggaggt agcttacctc ttattgcttc
     1381 agttgatagc tttgagatga tcagtgttgt cttggctata tatcaggatg caaaatacaa
     1441 agacctcggg atcgatccaa agaagtatga caccagggaa gccttaggaa aagtttgcac
     1501 tgtgctaaaa agcaaagcat ttgaaatgaa tgaagatcag gtgaagaaag ggaaagagta
     1561 tgctgctata cttagctcca gcaatcctaa tgctaaagga agtattgcta tggaacatta
     1621 cagtgaaact cttaacaagt tctatgaaat gttcggggtt aaaaaacaga caaaacttgc
     1681 agaacttgct taaaagcagt tgtaagttaa attataaaaa agcctataaa tatataaagc
     1741 tttctttatc tttattgctt gtgcttgctt agtgtgttaa atttaaaata agtgtgttta
     1801 attaaagttt gctttctgtg tgttgtgctt aataataaac aaaataacaa aaacaacaaa
     1861 aaacataaaa taaaaataag aaaaaataaa ataaaataaa ataaaataaa taaataaaaa
     1921 taaaaataaa ataaaaacaa aaaacaaaaa acaaaaaaca aaacaaaacc caaatttggc
     1981 caaaattgtc cctttcggga tctttttggg tttttgtttt ttaatttttt gttgttttta
     2041 tttcattttt tgatttttgt ttgattttgt ttcatttttt gatttttgtt tcattttgat
     2101 tttattttta tttttatttt tatttttatg gtttctatta aacaggagaa attcaaaaga
     2161 aatgacaaaa cagagaaata attatgagta aagaaagaaa ataaacataa cataattagg
     2221 aaaagctgga cacggcaaga ttacttgtac agctcgtcca tgccgagagt gatcccggcg
     2281 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
     2341 gcggactggg tgctcaggta gtggttgtcg ggcagcggca cggggccgtc gccgatgggg
     2401 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
     2461 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
     2521 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
     2581 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
     2641 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
     2701 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg
     2761 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
     2821 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
     2881 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
     2941 tcctcgccct tgctcaccat actagtgcac cgactcggtg ccactttttc aagttgataa
     3001 cggactagcc ttattttaac ttgctatttc tagctctaaa accgagaccg aggtccgagg
     3061 tctccactag ttatggttat tggtactgtg ttcttattac agtattgtga ttttctaagt
     3121 gaggtttgat taagaataaa attatgacac aattgctctg ggtcggcatg gcatctccac
     3181 ctcctcgcgg tccgacctgg gcatccgaag gaggacgtcg tccactcgga tggctaaggg
     3241 agagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     3301 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     3361 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     3421 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     3481 cgcgcgcggt gtcatctatg ttactagatc ggaattcaga ttgtcgtttc ccgccttcag
     3541 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
     3601 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
     3661 tgtgcatgcc aaccacagga gatctcagta aagcgaaaag tgccacctga acgaagcatc
     3721 tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa
     3781 tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag
     3841 aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     3901 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa
     3961 caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct
     4021 aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga
     4081 taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc
     4141 tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg
     4201 ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc
     4261 atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa
     4321 cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg
     4381 ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta
     4441 gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg
     4501 gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc
     4561 aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt
     4621 tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc
     4681 tatactttct agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag
     4741 cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat
     4801 atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc
     4861 ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg
     4921 tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg
     4981 ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt
     5041 tgatattgga tcatactaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     5101 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     5161 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     5221 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     5281 gcagattgta ctgagagtgc accatagatc aacgacatta ctatatatat aatataggaa
     5341 gcatttaata gaacagcatc gtaatatatg tgtactttgc agttatgacg ccagatggca
     5401 gtagtggaag atattcttta ttgaaaaata gcttgtcacc ttacgtacaa tcttgatccg
     5461 gagcttttct ttttttgccg attaagaatt aattcggtcg aaaaaagaaa aggagagggc
     5521 caagagggag ggcattggtg actattgagc acgtgagtat acgtgattaa gcacacaaag
     5581 gcagcttgga gtatgtctgt tattaatttc acaggtagtt ctggtccatt ggtgaaagtt
     5641 tgcggcttgc agagcacaga ggccgcagaa tgtgctctag attccgatgc tgacttgctg
     5701 ggtattatat gtgtgcccaa tagaaagaga acaattgacc cggttattgc aaggaaaatt
     5761 tcaagtcttg taaaagcata taaaaatagt tcaggcactc cgaaatactt ggttggcgtg
     5821 tttcgtaatc aacctaagga ggatgttttg gctctggtca atgattacgg cattgatatc
     5881 gtccaactgc atggagatga gtcgtggcaa gaataccaag agttcctcgg tttgccagtt
     5941 attaaaagac tcgtatttcc aaaagactgc aacatactac tcagtgcagc ttcacagaaa
     6001 cctcattcgt ttattccctt gtttgattca gaagcaggtg ggacaggtga acttttggat
     6061 tggaactcga tttctgactg ggttggaagg caagagagcc ccgaaagctt acattttatg
     6121 ttagctggtg gactgacgcc agaaaatgtt ggtgatgcgc ttagattaaa tggcgttatt
     6181 ggtgttgatg taagcggagg tgtggagaca aatggtgtaa aagactctaa caaaatagca
     6241 aatttcgtca aaaatgctaa gaaataggtt attactgagt agtatttatt taagtattgt
     6301 ttgtgcactt gccgatctat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     6361 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     6421 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     6481 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     6541 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     6601 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     6661 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     6721 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     6781 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag
     6841 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagatcct
     6901 tgctggctga acccccagcc ggaactgacc ccacaaggcc ctagcgtttg caatgcacca
     6961 ggtcatcatt gacccaggcg tgttccacca ggccgctgcc tcgcaactct tcgcaggctt
     7021 cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac atgaggcgga
     7081 aggtttccag cttgagcggg tacggctccc ggtgcgagct gaaatagtcg aacatccgtc
     7141 gggccgtcgg cgacagcttg cggtacttct cccatatgaa tttcgtgtag tggtcgccag
     7201 caaacagcac gacgatttcc tcgtcgatca ggacctggca acgggacgtt ttcttgccac
     7261 ggtccaggac gcggaagcgg tgcagcagcg acaccgattc caggtgccca acgcggtcgg
     7321 acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat
     7381 accggccatt gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg
     7441 gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc agttcgtcat
     7501 cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa
     7561 tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag
     7621 agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca
     7681 aggaaagctg catttccttg atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg
     7741 cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag
     7801 ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac
     7861 gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg gggagccagt tgcacgctgt
     7921 cgcgctcgat cttggccgta gcttgctggg ccatcgagcc gacggactgg aaggtttcgc
     7981 ggggcgcacg catgacggtg cggcttgcga tggtttcggc atcctcggcg gaaaaccccg
     8041 cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt caccctcctt
     8101 gcgggattgc cccgactcac gccggggcaa tgtgccctta ttcctgattt gacccgcctg
     8161 gtgccttggt gtccagataa tccaccttat cggcaatgaa gtcggtcccg tagaccgtct
     8221 ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg cacgaatacc agcgacccct
     8281 tgcccaaata cttgccgtgg gcctcggcct gagagccaaa acacttgatg cggaagaagt
     8341 cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat ctaggtacta aaacaattca
     8401 tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa
     8461 aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca
     8521 atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg
     8581 ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct
     8641 tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggggtgtct
     8701 tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg
     8761 gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag
     8821 agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac
     8881 tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc
     8941 cgttcaaggt gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc
     9001 ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat
     9061 aggttttcat tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct
     9121 tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc
     9181 atttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata
     9241 acaagacgaa ctccaattca ctgttccttg cattctaaaa ctttaaatac cagaaaacag
     9301 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga
     9361 aaccacaatt atgggtgatg ctgcaactcg agcgggccgg gagggttcga gaaggggggg
     9421 cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt
     9481 ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc
     9541 ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt
     9601 gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt
     9661 cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca
     9721 tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc
     9781 tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt
     9841 cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga
     9901 ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg
     9961 cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg
    10021 tctctagtgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
    10081 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
    10141 acaacttaat aacacattgc ggacgttttt aatgtactgg ggtggtttt
//
