LOCUS       sequence-389827-       11758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding nuclear-targeted iRFP713..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11758)
  AUTHORS   Matsuda K, Hirayama D, Hino N, Kuno S, Sakaue-Sawano A, Miyawaki A, 
            Matsuda M, Terai K
  TITLE     Knockout of all ErbB-family genes delineates their roles in 
            proliferation, survival, and migration.
  JOURNAL   J Cell Sci. 2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199.
  PUBMED    37519219
REFERENCE   2  (bases 1 to 11758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199."
COMMENT     Sequence Label: pCSIIpuro2-H2B-iRFP
FEATURES             Location/Qualifiers
     source          1..11758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2412..2529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2585..3747
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2816..3738
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3695..3715
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3803..4180
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4193..4202
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5267..5816
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5411..5428)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5638..5657
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5819..6418
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5819..5838)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6315..6335
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6453..7041
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6506..6526)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6924..6935)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7560..7740
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7766..7783)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7772..7996
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8042..8470
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8129..8148)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8339..8360
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8479..8499)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8484..8813
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8664..8799
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8726..8745
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8861..8908
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8927..9301
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9431..9552
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9468..9487)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9522..9541
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9601..9617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9601..9617)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9614..9636)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9625..9641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9649..9655,9656..9673,9674..9679))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9694..9715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9832..9849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10003..10591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10083..10102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10762..11553,11554..11622))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11385..11404
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11623..11727)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatctaca aatggcagta
     2401 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2461 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2521 caaaattttc gggtttatta cagggacagc agaaattcac tttgaattaa ttcaagcttc
     2581 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     2641 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     2701 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     2761 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     2821 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2881 aattacttcc acctggctcc agtacgtgat tcttgatccc gagctggagc caggggcggg
     2941 ccttgcgctt taggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     3001 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     3061 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     3121 gtaaatgcgg gccaggatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     3181 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     3241 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     3301 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     3361 gaaagatggc cgcttcccgg ccctgctcca gggggctcaa aatggaggac gcggcgctcg
     3421 ggagagcggg cgggtgagtc acccacacaa aggaaagggg cctttccgtc ctcagccgtc
     3481 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctggagc
     3541 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     3601 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     3661 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     3721 ttttttcttc catttcaggt gtcgtgaaca cgctaccggt ccgaattcgg cctcgaggcc
     3781 accgccaccg gatctcgcca ccatgccaga gccagcgaag tctgctcccg ccccgaaaaa
     3841 gggctccaag aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag
     3901 ccgcaaggag agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac
     3961 cggcatttcg tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg
     4021 catcgcaggt gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag
     4081 ggagatccag acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc
     4141 cgagggtact aaggccatca ccaagtacac cagcgctaag gatccaccgg tcgccaccat
     4201 ggccgaaggg agcgtggcaa gacagcccga tctgctgaca tgcgacgacg aaccaatcca
     4261 cattccagga gctatccagc ctcatggact gctgctggct ctggccgctg acatgactat
     4321 cgtggcaggc tctgataacc tgccagagct gaccgggctg gctatcggag cactgattgg
     4381 ccggtccgca gccgacgtgt tcgattctga gactcacaat agactgacca tcgccctggc
     4441 tgaacctggg gctgcagtgg gagccccaat taccgtcggc ttcacaatgc gaaaggacgc
     4501 tggctttatc gggtcctggc accgccatga tcagctgatt tttctggagc tggaaccacc
     4561 tcagagagac gtggcagagc cacaggcttt ctttcggaga acaaacagcg ccatcaggcg
     4621 cctgcaggcc gctgagactc tggaatccgc ttgcgcagcc gctgcacagg aagtgaggaa
     4681 gatcaccggg ttcgatcgcg tcatgatcta cagattcgcc tcagacttta gcggagaagt
     4741 gatcgcagaa gatcgctgtg ccgaggtcga aagtaaactg ggcctgcatt acccagcctc
     4801 aaccgtgcct gcacaggcac gacggctgta tacaatcaac cccgtgcgga tcattcccga
     4861 cattaattac agaccagtgc ccgtcacacc tgacctgaac ccagtgactg ggaggcccat
     4921 cgatctgagc ttcgccattc tgcgcagcgt gtcccctgtc cacctggagt ttatgaggaa
     4981 tatcggaatg catggcacca tgtctatcag tattctgaga ggcgaaaggc tgtgggggct
     5041 gatcgtgtgc caccatcgga caccttacta tgtggacctg gacggcaggc aggcttgtga
     5101 actggtggct caggtgctgg cttggcagat tggcgtgatg aaggaggggc atggcaccgg
     5161 cagcaccggc agcggcagca gcggccgcgg ctctagagga tccgttaact aacttaagct
     5221 agcaacggtt tccctctagc gggatcaatt ccgccccccc cccctaacgt tactggccga
     5281 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     5341 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     5401 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     5461 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     5521 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     5581 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     5641 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     5701 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     5761 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat
     5821 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     5881 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     5941 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6001 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     6061 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     6121 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     6181 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     6241 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6301 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6361 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgact
     6421 agagttaaca tcgagggatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     6481 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6541 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6601 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6661 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6721 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6781 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6841 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6901 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6961 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7021 ccctttgggc cgcctccccg catcgatacc gtcgagacct agaaaaacat ggagcaatca
     7081 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7141 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7201 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7261 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7321 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     7381 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     7441 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     7501 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     7561 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7621 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7681 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7741 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     7801 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     7861 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     7921 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     7981 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     8041 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8101 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8161 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8221 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8281 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8341 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8401 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8461 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     8521 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     8581 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8641 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     8701 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     8761 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     8821 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     8881 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     8941 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     9001 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     9061 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     9121 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     9181 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     9241 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     9301 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     9361 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     9421 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9481 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9541 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     9601 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9661 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9721 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9781 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9841 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9901 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9961 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10021 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10081 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10141 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10201 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10261 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10321 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10381 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10441 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10501 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10561 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10621 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10681 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10741 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10801 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10861 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10921 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10981 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11041 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11101 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11161 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11221 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11281 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11341 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11401 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11461 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11521 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11581 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11641 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11701 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
