LOCUS       sequence-389839-        8298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Easily insert deaminase of choice into BE4max using Golden Gate 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Rieffer AE, Chen Y, Salamango DJ, Moraes SN, Harris RS
  TITLE     APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels.
  JOURNAL   CRISPR J. 2023 Oct;6(5):430-446. doi: 10.1089/crispr.2023.0027. Epub
            2023 Sep 6.
  PUBMED    37672599
REFERENCE   2  (bases 1 to 8298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2023.0027"; journalName: "CRISPR J"; date: 
            "2023-10"; volume: "6"; issue: "5"; pages: "430-446"
COMMENT     Sequence Label: Modular BE4max for GG insertion
FEATURES             Location/Qualifiers
     source          1..8298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        130..333
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     284..304
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     375..394
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        375..393
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             443..463
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             581..4681
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEKNPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4712..4960
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             4991..5239
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5282..5302
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5311..5328
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5351..5368)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5357..5581
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5652..5668)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5652..5668)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5665..5687)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5676..5692
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5700..5706,5707..5724,5725..5730))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5745..5766
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5883..5900)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6054..6642)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6134..6153)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6813..7604,7605..7673))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7436..7455
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7674..7778)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7857..7876)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8048..8298,1..129)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
       61 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      121 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      181 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      241 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      301 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
      361 agatccgcgg ccgctaatac gactcactat agggagagcc gccaccatga aacggacagc
      421 cgacggaagc gagttcgagt caccaaagaa gaagcggaaa gtcgagacga tttaatgcgt
      481 ctcttctgga ggatctagcg gaggatcctc tggcagcgag acaccaggaa caagcgagtc
      541 agcaacacca gagagcagtg gcggcagcag cggcggcagc gacaagaagt acagcatcgg
      601 cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
      661 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
      721 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
      781 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
      841 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
      901 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
      961 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
     1021 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
     1081 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct
     1141 ggtgcagacc tacaaccagc tgttcgagaa aaaccccatc aacgccagcg gcgtggacgc
     1201 caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca
     1261 gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga gcctgggcct
     1321 gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa
     1381 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga
     1441 cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt
     1501 gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca
     1561 ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa
     1621 agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag
     1681 ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga
     1741 actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg
     1801 cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga
     1861 tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat
     1921 cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa
     1981 gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc
     2041 ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct
     2101 gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa
     2161 atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat
     2221 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
     2281 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
     2341 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
     2401 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
     2461 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
     2521 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
     2581 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
     2641 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
     2701 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
     2761 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
     2821 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
     2881 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
     2941 caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa
     3001 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
     3061 ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa
     3121 ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga
     3181 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa
     3241 cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct
     3301 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
     3361 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa
     3421 gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
     3481 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
     3541 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
     3601 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
     3661 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
     3721 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
     3781 aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag
     3841 catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
     3901 gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc
     3961 taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa
     4021 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
     4081 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
     4141 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
     4201 cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct
     4261 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
     4321 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga
     4381 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
     4441 caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa
     4501 tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga
     4561 caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat
     4621 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggtga
     4681 cagcggcggg agcggcggga gcggggggag cactaatctg agcgacatca ttgagaagga
     4741 gactgggaaa cagctggtca ttcaggagtc catcctgatg ctgcctgagg aggtggagga
     4801 agtgatcggc aacaagccag agtctgacat cctggtgcac accgcctacg acgagtccac
     4861 agatgagaat gtgatgctgc tgacctctga cgcccccgag tataagcctt gggccctggt
     4921 catccaggat tctaacggcg agaataagat caagatgctg agcggaggat ccggaggatc
     4981 tggaggcagc accaacctgt ctgacatcat cgagaaggag acaggcaagc agctggtcat
     5041 ccaggagagc atcctgatgc tgcccgaaga agtcgaagaa gtgatcggaa acaagcctga
     5101 gagcgatatc ctggtccata ccgcctacga cgagagtacc gacgaaaatg tgatgctgct
     5161 gacatccgac gccccagagt ataagccctg ggctctggtc atccaggatt ccaacggaga
     5221 gaacaaaatc aaaatgctgt ctggcggctc aaaaagaacc gccaacggca gcgaattcga
     5281 gcccaagaag aagaggaaag tctaaccggt catcatcacc atcaccattg agtttaaacc
     5341 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5401 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5461 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     5521 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     5581 gcttctgagg cggaaagaac cagctggggc tcgataccgt cgacctctag ctagagcttg
     5641 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5701 aacatacgag ccggaagcat aaagtgtaaa gcctagggtg cctaatgagt gagctaactc
     5761 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5821 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     5881 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     5941 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6001 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6061 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6121 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6181 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6241 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6301 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6361 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6421 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6481 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6541 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6601 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6661 tctacggggt ctgacactca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6721 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6781 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6841 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6901 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6961 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7021 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7081 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7141 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7201 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7261 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7321 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7381 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7441 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7501 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7561 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7621 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7681 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7741 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7801 cctgacgtcg acggatcggg agatcgatct cccgatcccc tagggtcgac tctcagtaca
     7861 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     7921 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     7981 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     8041 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     8101 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     8161 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     8221 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     8281 gtacatcaag tgtatcat
//
