LOCUS       sequence-389840-        8386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express AID(1-188) BE3-based base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Rieffer AE, Chen Y, Salamango DJ, Moraes SN, Harris RS
  TITLE     APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels.
  JOURNAL   CRISPR J. 2023 Oct;6(5):430-446. doi: 10.1089/crispr.2023.0027. Epub
            2023 Sep 6.
  PUBMED    37672599
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2023.0027"; journalName: "CRISPR J"; date: 
            "2023-10"; volume: "6"; issue: "5"; pages: "430-446"
COMMENT     Sequence Label: AID∆C-Cas9n-UGI-NLS
FEATURES             Location/Qualifiers
     source          1..8386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        223..426
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     377..397
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      471..480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1089..4487,4488..4490,4491..5087,5088,5089..5091,
                     5092..5093,5094..5096,5097..5189)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1089..1112,1113..1115,1116..5189)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5202..5450
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5463..5483
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5492..5509
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5532..5549)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5538..5762
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5833..5849)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5833..5849)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5846..5868)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5857..5873
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5881..5887,5888..5905,5906..5911))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5926..5947
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6064..6081)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6235..6823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6315..6334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6994..7785,7786..7854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7617..7636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7855..7959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8038..8057)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8229..8386,1..222)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
       61 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      121 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      181 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      241 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      301 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      361 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      421 agagctggtt tagtgaaccg tcagatccgc tagagatccg cggccgcgcc gccaccatgg
      481 acagcctctt gatgaaccgg aggaagtttc tttaccaatt caaaaatgtc cgctgggcta
      541 agggtcggcg tgagacctac ctgtgctacg tagtgaagag gcgtgacagt gctacatcct
      601 tttcactgga ctttggttat cttcgcaata agaacggctg ccacgtggaa ttgctcttcc
      661 tccgctacat ctcggactgg gacctagacc ctggccgctg ctaccgcgtc acctggttca
      721 cctcctggag cccctgctac gactgtgccc gacatgtggc cgactttctg cgagggaacc
      781 ccaacctcag tctgaggatc ttcaccgcgc gcctctactt ctgtgaggac cgcaaggctg
      841 agcccgaggg gctgcggcgg ctgcaccgcg ccggggtgca aatagccatc atgaccttca
      901 aagattattt ttactgctgg aatacttttg tagaaaacca tgaaagaact ttcaaagcct
      961 gggaagggct gcatgaaaat tcagttcgtc tctccagaca gcttcggcgc atccttttgc
     1021 ccctgtatga ggttgatgac agcggcagcg agactcccgg gacctcagag tccgccacac
     1081 ccgaaagtga taaaaagtat tctattggtt tagccatcgg cactaattcc gttggatggg
     1141 ctgtcataac cgatgaatac aaagtacctt caaagaaatt taaggtgttg gggaacacag
     1201 accgtcattc gattaaaaag aatcttatcg gtgccctcct attcgatagt ggcgaaacgg
     1261 cagaggcgac tcgcctgaaa cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa
     1321 tatgttactt acaagaaatt tttagcaatg agatggccaa agttgacgat tctttctttc
     1381 accgtttgga agagtccttc cttgtcgaag aggacaagaa acatgaacgg caccccatct
     1441 ttggaaacat agtagatgag gtggcatatc atgaaaagta cccaacgatt tatcacctca
     1501 gaaaaaagct agttgactca actgataaag cggacctgag gttaatctac ttggctcttg
     1561 cccatatgat aaagttccgt gggcactttc tcattgaggg tgatctaaat ccggacaact
     1621 cggatgtcga caaactgttc atccagttag tacaaaccta taatcagttg tttgaagaga
     1681 accctataaa tgcaagtggc gtggatgcga aggctattct tagcgcccgc ctctctaaat
     1741 cccgacggct agaaaacctg atcgcacaat tacccggaga gaagaaaaat gggttgttcg
     1801 gtaaccttat agcgctctca ctaggcctga caccaaattt taagtcgaac ttcgacttag
     1861 ctgaagatgc caaattgcag cttagtaagg acacgtacga tgacgatctc gacaatctac
     1921 tggcacaaat tggagatcag tatgcggact tatttttggc tgccaaaaac cttagcgatg
     1981 caatcctcct atctgacata ctgagagtta atactgagat taccaaggcg ccgttatccg
     2041 cttcaatgat caaaaggtac gatgaacatc accaagactt gacacttctc aaggccctag
     2101 tccgtcagca actgcctgag aaatataagg aaatattctt tgatcagtcg aaaaacgggt
     2161 acgcaggtta tattgacggc ggagcgagtc aagaggaatt ctacaagttt atcaaaccca
     2221 tattagagaa gatggatggg acggaagagt tgcttgtaaa actcaatcgc gaagatctac
     2281 tgcgaaagca gcggactttc gacaacggta gcattccaca tcaaatccac ttaggcgaat
     2341 tgcatgctat acttagaagg caggaggatt tttatccgtt cctcaaagac aatcgtgaaa
     2401 agattgagaa aatcctaacc tttcgcatac cttactatgt gggacccctg gcccgaggga
     2461 actctcggtt cgcatggatg acaagaaagt ccgaagaaac gattactcca tggaattttg
     2521 aggaagttgt cgataaaggt gcgtcagctc aatcgttcat cgagaggatg accaactttg
     2581 acaagaattt accgaacgaa aaagtattgc ctaagcacag tttactttac gagtatttca
     2641 cagtgtacaa tgaactcacg aaagttaagt atgtcactga gggcatgcgt aaacccgcct
     2701 ttctaagcgg agaacagaag aaagcaatag tagatctgtt attcaagacc aaccgcaaag
     2761 tgacagttaa gcaattgaaa gaggactact ttaagaaaat tgaatgcttc gattctgtcg
     2821 agatctccgg ggtagaagat cgatttaatg cgtcacttgg tacgtatcat gacctcctaa
     2881 agataattaa agataaggac ttcctggata acgaagagaa tgaagatatc ttagaagata
     2941 tagtgttgac tcttaccctc tttgaagatc gggaaatgat tgaggaaaga ctaaaaacat
     3001 acgctcacct gttcgacgat aaggttatga aacagttaaa gaggcgtcgc tatacgggct
     3061 ggggacgatt gtcgcggaaa cttatcaacg ggataagaga caagcaaagt ggtaaaacta
     3121 ttctcgattt tctaaagagc gacggcttcg ccaataggaa ctttatgcag ctgatccatg
     3181 atgactcttt aaccttcaaa gaggatatac aaaaggcaca ggtttccgga caaggggact
     3241 cattgcacga acatattgcg aatcttgctg gttcgccagc catcaaaaag ggcatactcc
     3301 agacagtcaa agtagtggat gagctagtta aggtcatggg acgtcacaaa ccggaaaaca
     3361 ttgtaatcga gatggcacgc gaaaatcaaa cgactcagaa ggggcaaaaa aacagtcgag
     3421 agcggatgaa gagaatagaa gagggtatta aagaactggg cagccagatc ttaaaggagc
     3481 atcctgtgga aaatacccaa ttgcagaacg agaaacttta cctctattac ctacaaaatg
     3541 gaagggacat gtatgttgat caggaactgg acataaaccg tttatctgat tacgacgtcg
     3601 atcacattgt accccaatcc tttttgaagg acgattcaat cgacaataaa gtgcttacac
     3661 gctcggataa gaaccgaggg aaaagtgaca atgttccaag cgaggaagtc gtaaagaaaa
     3721 tgaagaacta ttggcggcag ctcctaaatg cgaaactgat aacgcaaaga aagttcgata
     3781 acttaactaa agctgagagg ggtggcttgt ctgaacttga caaggccgga tttattaaac
     3841 gtcagctcgt ggaaacccgc caaatcacaa agcatgttgc acagatacta gattcccgaa
     3901 tgaatacgaa atacgacgag aacgataagc tgattcggga agtcaaagta atcactttaa
     3961 agtcaaaatt ggtgtcggac ttcagaaagg attttcaatt ctataaagtt agggagataa
     4021 ataactacca ccatgcgcac gacgcttatc ttaatgccgt cgtagggacc gcactcatta
     4081 agaaataccc gaagctagaa agtgagtttg tgtatggtga ttacaaagtt tatgacgtcc
     4141 gtaagatgat cgcgaaaagc gaacaggaga taggcaaggc tacagccaaa tacttctttt
     4201 attctaacat tatgaatttc tttaagacgg aaatcactct ggcaaacgga gagatacgca
     4261 aacgaccttt aattgaaacc aatggggaga caggtgaaat cgtatgggat aagggccggg
     4321 acttcgcgac ggtgagaaaa gttttgtcca tgccccaagt caacatagta aagaaaactg
     4381 aggtgcagac cggagggttt tcaaaggaat cgattcttcc aaaaaggaat agtgataagc
     4441 tcatcgctcg taaaaaggac tgggacccga aaaagtacgg tggcttcgat agccctacag
     4501 ttgcctattc tgtcctagta gtggcaaaag ttgagaaggg aaaatccaag aaactgaagt
     4561 cagtcaaaga attattgggg ataacgatta tggagcgctc gtcttttgaa aagaacccca
     4621 tcgacttcct tgaggcgaaa ggttacaagg aagtaaaaaa ggatctcata attaaactac
     4681 caaagtatag tctgtttgag ttagaaaatg gccgaaaacg gatgttggct agcgccggag
     4741 agcttcaaaa ggggaacgaa ctcgcactac cgtctaaata cgtgaatttc ctgtatttag
     4801 cgtcccatta cgagaagttg aaaggttcac ctgaagataa cgaacagaag caactttttg
     4861 ttgagcagca caaacattat ctcgacgaaa tcatagagca aatttcggaa ttcagtaaga
     4921 gagtcatcct agctgatgcc aatctggaca aagtattaag cgcatacaac aagcacaggg
     4981 ataaacccat acgtgagcag gcggaaaata ttatccattt gtttactctt accaacctcg
     5041 gcgctccagc cgcattcaag tattttgaca caacgataga tcgcaaacga tacacttcta
     5101 ccaaggaggt gctagacgcg acactgattc accaatccat cacgggatta tatgaaactc
     5161 ggatagattt gtcacagctt gggggtgact ctggtggttc tactaatctg tcagatatta
     5221 ttgaaaagga gaccggtaag caactggtta tccaggaatc catcctcatg ctcccagagg
     5281 aggtggaaga agtcattggg aacaagccgg aaagcgatat actcgtgcac accgcctacg
     5341 acgagagcac cgacgagaat gtcatgcttc tgactagcga cgcccctgaa tacaagcctt
     5401 gggctctggt catacaggat agcaacggtg agaacaagat taagatgctc tctggtggtt
     5461 ctcccaagaa gaagaggaaa gtctaaccgg tcatcatcac catcaccatt gagtttaaac
     5521 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5581 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5641 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5701 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5761 ggcttctgag gcggaaagaa ccagctgggg ctcgataccg tcgacctcta gctagagctt
     5821 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5881 caacatacga gccggaagca taaagtgtaa agcctagggt gcctaatgag tgagctaact
     5941 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6001 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6061 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6121 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6181 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6661 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7981 acctgacgtc gacggatcgg gagatcgatc tcccgatccc ctagggtcga ctctcagtac
     8041 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     8101 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     8161 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
     8221 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     8281 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     8341 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgtt
//
