LOCUS       sequence-389848-       12673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express eGFP fluorescence after Y93H correction and mCherry as 
            transfection control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Rieffer AE, Chen Y, Salamango DJ, Moraes SN, Harris RS
  TITLE     APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels.
  JOURNAL   CRISPR J. 2023 Oct;6(5):430-446. doi: 10.1089/crispr.2023.0027. Epub
            2023 Sep 6.
  PUBMED    37672599
REFERENCE   2  (bases 1 to 12673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2023.0027"; journalName: "CRISPR J"; date: 
            "2023-10"; volume: "6"; issue: "5"; pages: "430-446"
COMMENT     Sequence Label: eGFP Y93H TC editing reporter
FEATURES             Location/Qualifiers
     source          1..12673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             583..840
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2005..2238
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2423..2467
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2761..2787
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     enhancer        3104..3407
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3408..3611
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3562..3582
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3608..3632
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3648..4355
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3793..3811)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4061..4080
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4380..4433
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(4479..4500)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4740..4759)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5087..5108
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5181..5749
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5343..5360)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5570..5589
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5788..6807
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5788..6807
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_RNA        6967..7042
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     LTR             7130..7763
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9403..9420)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9574..10162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9654..9673)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10333..11124,11125..11193))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10956..10975
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11194..11298)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11366..11384
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11422..11444)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11544..11563
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acaagataac caagacacct cccaaggcta ccacaatggg ccgccctcca cgtgcacatg
       61 gccggaggaa ctgccatgtc ggaggtgcaa gcacacctgc gcatcagagt ccttggtgtg
      121 gagggaggga ccagcgcagc ttccagccat ccacctgatg aacagaacct agggaaagcc
      181 ccagttctac ttacaccagg aaaggctgga agggctaatt tggtcccaaa aaagacaaga
      241 gatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
      301 accagggcca gggatcagat atccactgac ctttggatgg tgcttcaagt tagtaccagt
      361 tgaaccagag caagtagaag aggccaatga aggagagaac aacagcttgt tacaccctat
      421 gagccagcat gggatggagg acccggaggg agaagtatta gtgtggaagt ttgacagcct
      481 cctagcattt cgtcacatgg cccgagagct gcatccggag tactacaaag actgctgaca
      541 tcgagctttc tacaagggac tttccgctgg ggactttcca gggaggtgtg gcctgggcgg
      601 gactggggag tggcgagccc tcagatgcta catataagca gctgcttttt gcctgtactg
      661 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      721 tgcttaagcc tcaataaagc ttgccttgag tgctcaaagt agtgtgtgcc cgtctgttgt
      781 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      841 gtggcgcccg aacagggact tgaaagcgaa agtaaagcca gaggagatct ctcgacgcag
      901 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      961 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcggtattaa
     1021 gcgggggaga attagataaa tgggaaaaaa ttcggttaag gccaggggga aagaaacaat
     1081 ataaactaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1141 gccttttaga gacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1201 agacaggatc agaagaactt agatcattat ataatacaat agcagtcctc tattgtgtgc
     1261 atcaaaggat agatgtaaaa gacaccaagg aagccttaga taagatagag gaagagcaaa
     1321 acaaaagtaa gaaaaaggca cagcaagcag cagctgacac aggaaacaac agccaggtca
     1381 gccaaaatta ccctatagtg cagaacctcc aggggcaaat ggtacatcag gccatatcac
     1441 ctagaacttt aaatcgacat agcagaatag gcgttactcg acagaggaga gcaagaaatg
     1501 gagccagtag atcctagact agagccctgg aagcatccag gaagtcagcc taaaactgct
     1561 tgtaccaatt gctattgtaa aaagtgttgc tttcattgcc aagtttgttt catgacaaaa
     1621 gccttaggca tctcctatgg caggaagaag cggagacagc gacgaagagc tcatcagaac
     1681 agtcagactc atcaagcttc tctatcaaag cagtaagtag tacatgggcg cgcccatgtg
     1741 gcaggaagta ggaaaagcaa tgtatgcccc tcccatcagt ggacaaatta gatgttcatc
     1801 aaatattact gggctgctat taacaagaga tggtggtaat aacaacaatg ggtccgagat
     1861 cttcagacct ggaggaggcg atatgaggga caattggaga agtgaattat ataaatataa
     1921 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     1981 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     2041 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctga
     2101 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     2161 actcacagtc tggggcatca aacagctcca ggcaagaatc ctggctgtgg aaagatacct
     2221 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     2281 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga ataacatgac
     2341 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     2401 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     2461 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     2521 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     2581 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaatcc cgaggggacc
     2641 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     2701 attagtgaac ggatccttag cacttatctg ggacgatctg cggagcctgt gcctcttcag
     2761 ctaccaccgc ttgagagact tactcttgat tgtaacgagg attgtggaac ttctgggacg
     2821 cagggggtgg gaagccctca aatattggtg gaatctccta caatattgga gtcaggagct
     2881 aaagaatagt gctgttagct tgctcaatgc cacagccata gcagtagctg aggggacaga
     2941 tagggttata gaagtagtac aaggagcttc tagagctatt cgccacatac ctagaagaat
     3001 aagacagggc ttggaaagga ttttgctata agatgggtgg cgcggccgct taatagtaat
     3061 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     3121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3181 atgttcccat agtaacgcca attgggactt tccattgacg tcaatgggtg gagtatttac
     3241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     3601 taagcagagc tcgtttagtg aaccgtcaga tccagctggc taccggtatg gtgagcaagg
     3661 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     3721 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     3781 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     3841 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     3901 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     3961 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     4021 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     4081 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     4141 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     4201 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     4261 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     4321 gccactccac cggcggcatg gacgagctgt acaagcccgg tgcagatctc gagtacaagg
     4381 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatggtca
     4441 gtaaaggtga agaactgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     4501 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4561 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4621 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     4681 acttcttcaa gtccgccatg cccgaaggtc acgtccagga gcggaccatc ttcttcaagg
     4741 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4801 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4861 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     4921 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4981 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     5041 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     5101 agttcgtgac cgccgccggg atcactctcg gtatggatga attatataaa taacgtacgc
     5161 ttaattaacg gatccggaat tccgctcccc cccccctaac gttactggcc gaagccgctt
     5221 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     5281 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     5341 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     5401 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     5461 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     5521 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     5581 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     5641 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     5701 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa
     5761 cccgtaccaa agatggatag atccggaaag cctgaactca ccgcgacgtc tgtcgagaag
     5821 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     5881 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     5941 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     6001 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
     6061 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     6121 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     6181 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     6241 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     6301 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     6361 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     6421 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     6481 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     6541 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     6601 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     6661 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     6721 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     6781 agcactcgtc cgagggcaaa ggaatagagt agatgccgac cgaacaaggc atgctcttca
     6841 acctcaataa cgttatatcc tgattcacgt aatatttttg gggtaaattt agttcctgtt
     6901 ccattaactg cgctaaaaat aatttttaaa tcttttttag cttcttgctc ttttttgtac
     6961 gtctctgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     7021 aaaagtggca ccgagtcggt gcttttttga attcgttcgg tacctttaag accaatgact
     7081 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7141 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     7201 ttccctgatt ggcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     7261 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     7321 aacaccagct tgttacaccc tgtgagcctg catggaatgg atgaccctga gagagaagtg
     7381 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     7441 gagtacttca agaactgctg acatcgagct tgctacaagg gactttccgc tggggacttt
     7501 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatg ctgcatataa
     7561 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7621 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7681 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7741 gtcagtgtgg aaaatctcta gcacccagga ggtagaggtt gcagtgagcc aagatcgcgc
     7801 cactgcattc cagcctgggc aagaaaacaa gactgtctaa aataataata ataagttaag
     7861 ggtattaaat atatttatac atggaggtca taaaaatata tatatttggg ctgggcgcag
     7921 tggctcacac ctgcgcccgg ccctttggga ggccgaggca ggtggatcac ctgagtttgg
     7981 gagttccaga ccagcctgac caacatggag aaaccccttc tctgtgtatt tttagtagat
     8041 tttattttat gtgtatttta ttcacaggta tttctggaaa actgaaactg tttttcctct
     8101 actctgatac cacaagaatc atcagcacag aggaagactt ctgtgatcaa atgtggtggg
     8161 agagggaggt tttcaccagc acatgagcag tcagttctgc cgcagactcg gcgggtgtcc
     8221 ttcggttcag ttccaacacc gcctgcctgg agagaggtca gaccacaggg tgagggctca
     8281 gtccccaaga cataaacacc caagacataa acacccaaca ggtccacccc gcctgctgcc
     8341 caggcagagc cgattcacca agacgggaat taggatagag aaagagtaag tcacacagag
     8401 ccggctgtgc gggagaacgg agttctatta tgactcaaat cagtctcccc aagcattcgg
     8461 ggatcagagt ttttaaggat aacttagtgt gtagggggcc agtgagttgg agatgaaagc
     8521 gtagggagtc gaaggtgtcc ttttgcgccg agtcagttcc tgggtggggg ccacaagatc
     8581 ggatgagcca gtttatcaat ccgggggtgc cagctgatcc atggagtgca gggtctgcaa
     8641 aatatctcaa gcactgattg atcttaggtt ttacaatagt gatgttaccc caggaacaat
     8701 ttggggaagg tcagaatctt gtagcctgta gctgcatgac tcctaaacca taatttcttt
     8761 tttgtttttt tttttttatt tttgagacag ggtctcactc tgtcacctag gctggagtgc
     8821 agtggtgcaa tcacagctca ctgcagcctc aacgtcgtaa gctcaagcga tcctcccacc
     8881 tcagcctgcc tggtagctga gactacaagc gacgccccag ttaatttttg tatttttggt
     8941 agaggcagcg ttttgccgtg tggccctggc tggtctcgaa ctcctgggct caagtgatcc
     9001 agcctcagcc tcccaaagtg ctgggacaac cggggccagt cactgcacct ggccctaaac
     9061 cataatttct aatcttttgg ctaatttgtt agtcctacaa aggcagtcta gtccccaggc
     9121 aaaaaggggg tttgtttcgg gaaagggctg ttactgtctt tgtttcaaac tataaactaa
     9181 gttcctccta aacttagttc ggcctacacc caggaatgaa caaggagagc ttggaggtta
     9241 gaagcacgat ggaattggtt aggtcagatc tctttcactg tctgagttat aattttgcaa
     9301 tggtggttca aagactgccc gcttctgaca ccagtcgctg cattaatgaa tcggccaacg
     9361 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     9421 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9481 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9541 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9601 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9661 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9721 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9781 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9841 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9901 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9961 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    10021 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    10081 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    10141 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    10201 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    10261 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    10321 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    10381 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    10441 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10501 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    10561 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    10621 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    10681 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    10741 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10801 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10861 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10921 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10981 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    11041 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    11101 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    11161 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    11221 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    11281 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    11341 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    11401 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    11461 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    11521 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    11581 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
    11641 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    11701 ggggaggcag agattgcagt aagctgagat cgcagcactg cactccagcc tgggcgacag
    11761 agtaagactc tgtctcaaaa ataaaataaa taaatcaatc agatattcca atcttttcct
    11821 ttatttattt atttattttc tattttggaa acacagtcct tccttattcc agaattacac
    11881 atatattcta tttttcttta tatgctccag ttttttttag accttcacct gaaatgtgtg
    11941 tatacaaaat ctaggccagt ccagcagagc ctaaaggtaa aaaataaaat aataaaaaat
    12001 aaataaaatc tagctcactc cttcacatca aaatggagat acagctgtta gcattaaata
    12061 ccaaataacc catcttgtcc tcaataattt taagcgcctc tctccaccac atctaactcc
    12121 tgtcaaaggc atgtgcccct tccgggcgct ctgctgtgct gccaaccaac tggcatgtgg
    12181 actctgcagg gtccctaact gccaagcccc acagtgtgcc ctgaggctgc cccttccttc
    12241 tagcggctgc ccccactcgg ctttgctttc cctagtttca gttacttgcg ttcagccaag
    12301 gtctgaaact aggtgcgcac agagcggtaa gactgcgaga gaaagagacc agctttacag
    12361 ggggtttatc acagtgcacc ctgacagtcg tcagcctcac agggggttta tcacattgca
    12421 ccctgacagt cgtcagcctc acagggggtt tatcacagtg cacccttaca atcattccat
    12481 ttgattcaca atttttttag tctctactgt gcctaacttg taagttaaat ttgatcagag
    12541 gtgtgttccc agaggggaaa acagtatata cagggttcag tactatcgca tttcaggcct
    12601 ccacctgggt cttggaatgt gtcccccgag gggtgatgac tacctcagtt ggatctccac
    12661 aggtcacagt gac
//
