LOCUS       sequence-389851-       12345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The lentiviral vector for NIR-GECO2(I426C) and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12345)
  TITLE     Plasmids for KOBACHI mice project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCSIIbleo-6507-P2A-EGFP
FEATURES             Location/Qualifiers
     source          1..12345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2412..2529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2585..3747
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2816..3738
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3695..3715
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5279..5335
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5345..5347,5348..5350,5351..5863)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(5390..5411)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5651..5670)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5998..6019
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6078..6627
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6222..6239)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6449..6468
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6630..7004
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    7040..7628
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7093..7113)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7511..7522)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8147..8327
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8353..8370)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8359..8583
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8629..9057
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8716..8735)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8926..8947
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9066..9086)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9071..9400
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9251..9386
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9313..9332
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9448..9495
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9514..9888
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10018..10139
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10055..10074)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10109..10128
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10188..10204)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10188..10204)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10201..10223)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10212..10228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10236..10242,10243..10260,10261..10266))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10281..10302
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10419..10436)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10590..11178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10670..10689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11349..12140,12141..12209))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11972..11991
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12210..12314)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatctaca aatggcagta
     2401 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2461 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2521 caaaattttc gggtttatta cagggacagc agaaattcac tttgaattaa ttcaagcttc
     2581 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     2641 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     2701 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     2761 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     2821 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2881 aattacttcc acctggctcc agtacgtgat tcttgatccc gagctggagc caggggcggg
     2941 ccttgcgctt taggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     3001 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     3061 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     3121 gtaaatgcgg gccaggatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     3181 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     3241 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     3301 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     3361 gaaagatggc cgcttcccgg ccctgctcca gggggctcaa aatggaggac gcggcgctcg
     3421 ggagagcggg cgggtgagtc acccacacaa aggaaagggg cctttccgtc ctcagccgtc
     3481 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctggagc
     3541 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     3601 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     3661 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     3721 ttttttcttc catttcaggt gtcgtgaaca cgctaccggt ctcgacgaat tcgccaccat
     3781 gtcggtaccg ctgactacct cagcattcgg ccacgcgttt ctggctaact gtgaacgtga
     3841 gcagatccac ctggcgggct ccattcagcc gcacggtatc ctgctggctg ttaaagagcc
     3901 tgacaacgtg gtgatccagg cttctattaa cgctgcggag ttcctgaaca ccaactttgt
     3961 tgttggccgt ccgctgcgtg acctgggcgg cgatctgcct ttgcagatcc tgccgcacct
     4021 gaacggcccg ctgcacctgg ctccgatgac cctgcgttgt actgtgggtt ctccgccgcg
     4081 tcgtgtggac tgtaccattc atcgaccgtc taacggcggc ctgatcgtag aactggaacc
     4141 agcaaccaag gccactaaca ttgcgccggc tctggtcggt gcgcttcatc gtatcacttc
     4201 ttcatcctcc ctgatgggcc tgtgtgacga aaccgcgact attttccgtg agattactgg
     4261 tttcgaccgt gtgatggtaa tgcgtctcgg cgcgcttgac gatctgactg aagagcagat
     4321 cgcagagatt aaagaggctt tctccctatt tgacaaggac ggggacggga cgataacaac
     4381 caaggagctg gggacggtgt tccggtctct ggggcagaac cccacagaag cagagctgca
     4441 ggacatgatc aatgaagtag atgccgatgg cgacggcata ttcgacttcc ctgagttcct
     4501 gacgatgatg gcaaggaaaa tgaatgacac agacagtgaa gaggaaatta gaggagcgtt
     4561 ccgcgtgttt gataaggacg gcaatggcta catcggcgca gcagagcttc gccacgtgat
     4621 gacagacctt ggtgagaagt taactgatga ggaggttgat gaaatgatca gggtagcaga
     4681 caacgatggg gatggtcagg taaactacga agagtttgta caaatgatga cagcgaaggg
     4741 tggcggaggt tctgtagatt catcacgtcg taagtggaat aaggcaggtc acgcagtcag
     4801 agctataggt cggctgagct cgcgtaggca tgatttgctg tccgaatgtc gtcgtgcgga
     4861 cctggaagcg ttcctaggta accgctaccc ggcgtctact attccgcaga tcgctcgtcg
     4921 cctgtacgaa ctcaaccgtg ttcgcctgct ggtagatgtg aactatactc cggttccgct
     4981 agagccgcgc atcagcccgc tgaacggtcg tgatctggat atgtccctgt cttgcctgcg
     5041 atcgatgtcc ccgtgccacc agaaatacat gcaggacatg ggcgttggcg caaccctggt
     5101 ttgctctctg atggtgtctg gtcgtctgtg gggtctgatc gtttgccacc actacgaacc
     5161 gcgctacgtt ccgtcccaca ttcgcgctgc tggcgaagcg ctggcggaag catgtgcgaa
     5221 ccgcatcgcg acgctggaga gctttgcaca gtctcagtcc aaactcgagg gaagcggagc
     5281 gacgaatttt agtctactga aacaagcggg agacgtggag gaaaaccctg gacctggcgg
     5341 ccgcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     5401 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     5461 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     5521 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     5581 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     5641 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     5701 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     5761 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     5821 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     5881 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     5941 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     6001 ggtcctgctg gagttcgtga ccgccgcctc tagcaacggt ttccctctag cgggatcaat
     6061 tccgcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     6121 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     6181 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     6241 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     6301 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     6361 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     6421 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     6481 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     6541 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     6601 ggttttcctt tgaaaaacac gataatacca tggccaagtt gaccagtgcc gttccggtgc
     6661 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
     6721 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
     6781 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
     6841 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
     6901 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
     6961 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgaactaga gttaacatcg
     7021 agggatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7081 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7141 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7201 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7261 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7321 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7381 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7441 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7501 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7561 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7621 ctccccgcat cgataccgtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     7681 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     7741 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7801 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     7861 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     7921 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     7981 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     8041 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     8101 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     8161 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8221 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8281 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
     8341 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     8401 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     8461 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     8521 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     8581 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     8641 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     8701 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     8761 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     8821 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     8881 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     8941 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     9001 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     9061 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     9121 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     9181 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     9241 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9301 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     9361 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     9421 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     9481 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     9541 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     9601 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     9661 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     9721 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     9781 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     9841 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     9901 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     9961 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
    10021 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10081 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10141 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
    10201 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
    10261 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
    10321 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
    10381 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
    10441 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    10501 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    10561 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    10621 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    10681 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    10741 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    10801 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    10861 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    10921 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    10981 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    11041 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    11101 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    11161 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    11221 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    11281 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    11341 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    11401 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    11461 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    11521 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    11581 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    11641 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    11701 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    11761 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    11821 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    11881 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    11941 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    12001 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    12061 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    12121 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    12181 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    12241 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    12301 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
