LOCUS       sequence-389859-       12673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express eGFP fluorescence after Y93H correction and mCherry as 
            transfection control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Rieffer AE, Chen Y, Salamango DJ, Moraes SN, Harris RS
  TITLE     APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels.
  JOURNAL   CRISPR J. 2023 Oct;6(5):430-446. doi: 10.1089/crispr.2023.0027. Epub
            2023 Sep 6.
  PUBMED    37672599
REFERENCE   2  (bases 1 to 12673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2023.0027"; journalName: "CRISPR J"; date: 
            "2023-10"; volume: "6"; issue: "5"; pages: "430-446"
COMMENT     Sequence Label: eGFP Y93H AC editing reporter
FEATURES             Location/Qualifiers
     source          1..12673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1799..2032
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2217..2261
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2555..2581
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     enhancer        2898..3201
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3202..3405
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3356..3376
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3402..3426
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3442..4149
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3587..3605)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3855..3874
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4174..4227
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(4273..4294)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4534..4553)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4881..4902
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4975..5543
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5137..5154)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5364..5383
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5582..6601
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5582..6601
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_RNA        6761..6836
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     LTR             6924..7557
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9197..9214)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9368..9956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9448..9467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10127..10918,10919..10987))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10750..10769
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10988..11092)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11160..11178
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11216..11238)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11338..11357
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatcg acatagcaga
     1261 ataggcgtta ctcgacagag gagagcaaga aatggagcca gtagatccta gactagagcc
     1321 ctggaagcat ccaggaagtc agcctaaaac tgcttgtacc aattgctatt gtaaaaagtg
     1381 ttgctttcat tgccaagttt gtttcatgac aaaagcctta ggcatctcct atggcaggaa
     1441 gaagcggaga cagcgacgaa gagctcatca gaacagtcag actcatcaag cttctctatc
     1501 aaagcagtaa gtagtacatg ggcgcgccca tgtggcagga agtaggaaaa gcaatgtatg
     1561 cccctcccat cagtggacaa attagatgtt catcaaatat tactgggctg ctattaacaa
     1621 gagatggtgg taataacaac aatgggtccg agatcttcag acctggagga ggcgatatga
     1681 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1741 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1801 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1861 cgctgacggt acaggccaga caattattgt ctgatatagt gcagcagcag aacaatttgc
     1921 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaaacagc
     1981 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     2041 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     2101 ataaatctct ggaacagatt tggaataaca tgacctggat ggagtgggac agagaaatta
     2161 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2221 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2281 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2341 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2401 cgtttcagac ccacctccca atcccgaggg gacccgacag gcccgaagga atagaagaag
     2461 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcc ttagcactta
     2521 tctgggacga tctgcggagc ctgtgcctct tcagctacca ccgcttgaga gacttactct
     2581 tgattgtaac gaggattgtg gaacttctgg gacgcagggg gtgggaagcc ctcaaatatt
     2641 ggtggaatct cctacaatat tggagtcagg agctaaagaa tagtgctgtt agcttgctca
     2701 atgccacagc catagcagta gctgagggga cagatagggt tatagaagta gtacaaggag
     2761 cttctagagc tattcgccac atacctagaa gaataagaca gggcttggaa aggattttgc
     2821 tataagatgg gtggcgcggc cgcttaatag taatcaatta cggggtcatt agttcatagc
     2881 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     2941 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaattggg
     3001 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     3061 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     3121 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     3181 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     3241 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     3301 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     3361 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     3421 cagatccagc tggctaccgg tatggtgagc aagggcgagg aggataacat ggccatcatc
     3481 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     3541 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     3601 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     3661 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     3721 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     3781 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     3841 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     3901 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     3961 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     4021 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     4081 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     4141 ctgtacaagc ccggtgcaga tctcgagtac aaggagggca gaggaagtct tctaacatgc
     4201 ggtgacgtgg aggagaatcc cggccctatg gtcagtaaag gtgaagaact gttcaccggg
     4261 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     4321 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     4381 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     4441 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     4501 ggacacgtcc aggagcggac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     4561 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     4621 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     4681 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     4741 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     4801 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     4861 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     4921 ctcggtatgg atgaattata taaataacgt acgcttaatt aacggatccg gaattccgct
     4981 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     5041 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     5101 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     5161 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     5221 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     5281 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     5341 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     5401 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     5461 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     5521 tcctttgaaa aacacgatga taagcttgcc acaacccgta ccaaagatgg atagatccgg
     5581 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     5641 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     5701 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     5761 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     5821 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     5881 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     5941 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     6001 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     6061 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     6121 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     6181 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     6241 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     6301 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     6361 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     6421 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     6481 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     6541 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
     6601 gagtagatgc cgaccgaaca aggcatgctc ttcaacctca ataacgttat atcctgattc
     6661 acgtaatatt tttggggtaa atttagttcc tgttccatta actgcgctaa aaataatttt
     6721 taaatctttt ttagcttctt gctctttttt gtacgtctct gttttagagc tagaaatagc
     6781 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     6841 ttgaattcgt tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6901 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaaagaa gacaagatat
     6961 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     7021 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     7081 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     7141 cctgcatgga atggatgacc ctgagagaga agtgttagag tggaggtttg acagccgcct
     7201 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgacatcg
     7261 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     7321 tggggagtgg cgagccctca gatgctgcat ataagcagct gctttttgcc tgtactgggt
     7381 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7441 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7501 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaccc
     7561 aggaggtaga ggttgcagtg agccaagatc gcgccactgc attccagcct gggcaagaaa
     7621 acaagactgt ctaaaataat aataataagt taagggtatt aaatatattt atacatggag
     7681 gtcataaaaa tatatatatt tgggctgggc gcagtggctc acacctgcgc ccggcccttt
     7741 gggaggccga ggcaggtgga tcacctgagt ttgggagttc cagaccagcc tgaccaacat
     7801 ggagaaaccc cttctctgtg tatttttagt agattttatt ttatgtgtat tttattcaca
     7861 ggtatttctg gaaaactgaa actgtttttc ctctactctg ataccacaag aatcatcagc
     7921 acagaggaag acttctgtga tcaaatgtgg tgggagaggg aggttttcac cagcacatga
     7981 gcagtcagtt ctgccgcaga ctcggcgggt gtccttcggt tcagttccaa caccgcctgc
     8041 ctggagagag gtcagaccac agggtgaggg ctcagtcccc aagacataaa cacccaagac
     8101 ataaacaccc aacaggtcca ccccgcctgc tgcccaggca gagccgattc accaagacgg
     8161 gaattaggat agagaaagag taagtcacac agagccggct gtgcgggaga acggagttct
     8221 attatgactc aaatcagtct ccccaagcat tcggggatca gagtttttaa ggataactta
     8281 gtgtgtaggg ggccagtgag ttggagatga aagcgtaggg agtcgaaggt gtccttttgc
     8341 gccgagtcag ttcctgggtg ggggccacaa gatcggatga gccagtttat caatccgggg
     8401 gtgccagctg atccatggag tgcagggtct gcaaaatatc tcaagcactg attgatctta
     8461 ggttttacaa tagtgatgtt accccaggaa caatttgggg aaggtcagaa tcttgtagcc
     8521 tgtagctgca tgactcctaa accataattt cttttttgtt tttttttttt tatttttgag
     8581 acagggtctc actctgtcac ctaggctgga gtgcagtggt gcaatcacag ctcactgcag
     8641 cctcaacgtc gtaagctcaa gcgatcctcc cacctcagcc tgcctggtag ctgagactac
     8701 aagcgacgcc ccagttaatt tttgtatttt tggtagaggc agcgttttgc cgtgtggccc
     8761 tggctggtct cgaactcctg ggctcaagtg atccagcctc agcctcccaa agtgctggga
     8821 caaccggggc cagtcactgc acctggccct aaaccataat ttctaatctt ttggctaatt
     8881 tgttagtcct acaaaggcag tctagtcccc aggcaaaaag ggggtttgtt tcgggaaagg
     8941 gctgttactg tctttgtttc aaactataaa ctaagttcct cctaaactta gttcggccta
     9001 cacccaggaa tgaacaagga gagcttggag gttagaagca cgatggaatt ggttaggtca
     9061 gatctctttc actgtctgag ttataatttt gcaatggtgg ttcaaagact gcccgcttct
     9121 gacaccagtc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9181 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9241 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9301 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9361 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9421 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9481 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9541 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9601 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9661 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9721 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9781 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9841 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9901 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9961 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10021 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10081 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10141 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10201 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10261 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10321 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10381 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10441 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10501 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10561 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10621 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10681 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10741 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10801 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10861 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10921 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10981 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    11041 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11101 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    11161 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
    11221 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    11281 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
    11341 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
    11401 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
    11461 ggcgatcggt gcgggcctct tcgctattac gccaggggag gcagagattg cagtaagctg
    11521 agatcgcagc actgcactcc agcctgggcg acagagtaag actctgtctc aaaaataaaa
    11581 taaataaatc aatcagatat tccaatcttt tcctttattt atttatttat tttctatttt
    11641 ggaaacacag tccttcctta ttccagaatt acacatatat tctatttttc tttatatgct
    11701 ccagtttttt ttagaccttc acctgaaatg tgtgtataca aaatctaggc cagtccagca
    11761 gagcctaaag gtaaaaaata aaataataaa aaataaataa aatctagctc actccttcac
    11821 atcaaaatgg agatacagct gttagcatta aataccaaat aacccatctt gtcctcaata
    11881 attttaagcg cctctctcca ccacatctaa ctcctgtcaa aggcatgtgc cccttccggg
    11941 cgctctgctg tgctgccaac caactggcat gtggactctg cagggtccct aactgccaag
    12001 ccccacagtg tgccctgagg ctgccccttc cttctagcgg ctgcccccac tcggctttgc
    12061 tttccctagt ttcagttact tgcgttcagc caaggtctga aactaggtgc gcacagagcg
    12121 gtaagactgc gagagaaaga gaccagcttt acagggggtt tatcacagtg caccctgaca
    12181 gtcgtcagcc tcacaggggg tttatcacat tgcaccctga cagtcgtcag cctcacaggg
    12241 ggtttatcac agtgcaccct tacaatcatt ccatttgatt cacaattttt ttagtctcta
    12301 ctgtgcctaa cttgtaagtt aaatttgatc agaggtgtgt tcccagaggg gaaaacagta
    12361 tatacagggt tcagtactat cgcatttcag gcctccacct gggtcttgga atgtgtcccc
    12421 cgaggggtga tgactacctc agttggatct ccacaggtca cagtgacaca agataaccaa
    12481 gacacctccc aaggctacca caatgggccg ccctccacgt gcacatggcc ggaggaactg
    12541 ccatgtcgga ggtgcaagca cacctgcgca tcagagtcct tggtgtggag ggagggacca
    12601 gcgcagcttc cagccatcca cctgatgaac agaacctagg gaaagcccca gttctactta
    12661 caccaggaaa ggc
//
