LOCUS       sequence-38986-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-F1-GCT
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             17..37
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             38..127
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 atctgccacc atggctccaa agaagaagcg taaggtatac ccatacgatg ttcctgacta
       61 tgcgggctat ccctatgacg tcccggacta tgcaggatcg tatccatatg acgttccaga
      121 ttacgctgct catggtacca agccctataa atgtcctgaa tgtggcaagt ccttctcgca
      181 ccgtcagtcc ctcactcgtc accaacgtac gcacaccggc gaaaaaccat atgcttgccc
      241 tgtcgagtcc tgtgaccgcc gcttcagtca ccgtcagtcc ctcactcgtc acatccggat
      301 tcacacaggc cagaagccct tccagtgtcg catctgcctg aggaacttca gccagtctgg
      361 tgctctcacc cgtcacatcc gcacccacac aggcgagaag ccttttgcat gcgacatttg
      421 cggcaggaag ttcgcgctta gtttcaacct cacccgccac accaagatcc acacaggtgg
      481 atccgcggct gcgcgtgcgc tagtcaaaag tgaactggag gagaagaaat ctgaacttcg
      541 tcataaattg aaatatgtgc ctcatgaata tattgaatta attgaaattg ccagaaattc
      601 cactcaggat agaattcttg aaatgaaggt aatggaattt tttatgaaag tttatggata
      661 tagaggtaaa catttgggtg gatcaaggaa accggacgga gcaatttata ctgtcggatc
      721 tcctattgat tacggtgtga tcgtggatac taaagcttat agcggaggtt ataatctgcc
      781 aattggccaa gcagatgaaa tgcaacgata tgtcaaagaa aatcaaacac gaaacaaaca
      841 tatcaaccct aatga
//
