LOCUS       sequence-389940-        8035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsGIGYF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Peter D, Weber R, Sandmeir F, Wohlbold L, Helms S, Bawankar P, 
            Valkov E, Igreja C, Izaurralde E
  TITLE     GIGYF1/2 proteins use auxiliary sequences to selectively bind to 
            4EHP and repress target mRNA expression.
  JOURNAL   Genes Dev. 2017 Jun 1;31(11):1147-1161. doi: 10.1101/gad.299420.117.
            Epub 2017 Jul 11.
  PUBMED    28698298
REFERENCE   2  (bases 1 to 8035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.299420.117"; journalName: "Genes Dev"; date: 
            "2017-06-1- 1"; volume: "31"; issue: "11"; pages: "1147-1161"
COMMENT     Sequence Label: pLNHA-C1-HsGIGYF2_AE
FEATURES             Location/Qualifiers
     source          1..8035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     553..572
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        553..571
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      599..608
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             605..670
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             695..721
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2810..2821
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    4763..4884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4800..4819)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4854..4873
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4891..5346)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5028..5049)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5240..5259
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5373..5477
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5479..5836
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5479..5670
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5502..5522)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5687..5822
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5871..6665
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5871..6665
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5925..5944)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6535..6554
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6853..6872)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6897..6944
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7273..7861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7762..7781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      541 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      601 caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
      661 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
      721 tcctccatct cgagctatgg cagcggaaac gcagacactg aactttgggc ctgaatggct
      781 ccgagctctg tccagtggtg ggagtattac atcccctcct ctttctccag cattgccgaa
      841 gtataaatta gcagattatc gttacggcag agaagaaatg ttagcacttt tccttaaaga
      901 caacaagata ccttcagacc ttctggataa agaatttctg cctatcctcc aggaggaacc
      961 ccttccacca ttggctctgg taccctttac agaagaagaa cagagaaact tttccatgtc
     1021 tgtaaatagt gctgctgtcc tgcgattgac aggacgagga ggaggaggaa cagtggtggg
     1081 ggctcctaga ggtcgaagtt cttcaagagg gcgaggcaga ggcagaggtg aatgtggttt
     1141 ctaccaaaga agttttgatg aagtagaggg tgtttttggt cgaggaggtg gcagagaaat
     1201 gcatagatcg cagagctggg aggaaagggg tgacagacgt tttgaaaaac caggacgaaa
     1261 agatgtaggg agaccaaatt ttgaggaagg tggaccaaca tcagtaggga gaaagcatga
     1321 atttatacgc tcagaaagtg aaaattggcg catctttaga gaggaacaaa atggagaaga
     1381 tgaagatgga ggttggcgac tagctggatc aaggagggat ggagagaggt ggcgacctca
     1441 cagtcctgat ggccctcgtt ctgcaggctg gcgggaacac atggaacgac gtcggaggtt
     1501 tgagtttgat tttcgagata gagatgatga acggggttac cgaagggttc gctctggcag
     1561 tgggagcata gatgatgaca gggatagctt gcccgaatgg tgcttagagg atgctgaaga
     1621 agaaatgggt acatttgact catctggagc attcctttct ctaaaaaaag tacagaaaga
     1681 gcctattcca gaagagcagg agatggactt ccggcctgtg gacgaagggg aggagtgctc
     1741 tgactctgag ggtagccata atgaagaggc caaagaaccc gataagacaa ataagaaaga
     1801 aggagagaaa acagatagag taggagttga agctagtgag gaaactcccc agacctcatc
     1861 atcatctgct agaccaggta ctccttcaga ccatcagtct caggaagcat cacagtttga
     1921 gaggaaagat gaaccaaaaa ctgagcaaac ggaaaaagct gaagaggaga ctcggatgga
     1981 aaatagtcta ccagccaaag tgcccagcag aggggatgaa atggttgctg atgtccagca
     2041 gcccctgtcg cagattcctt cagatacagc ctctcctctt ctcatacttc cacctcctgt
     2101 tcccaatcct agtcctactc tccggccagt tgaaacacca gttgtaggtg ctcctggtat
     2161 gggcagtgtt tccacagaac ctgatgatga agaaggtctc aaacatttgg agcagcaagc
     2221 tgagaaaatg gtggcttatc tccaagacag tgcactagat gatgaaagat tggcatcaaa
     2281 actgcaagag cacagagcta aaggagtgtc gattccattg atgcatgaag caatgcagaa
     2341 gtggtattac aaagatcctc agggagaaat tcaaggtccc ttcaataatc aggagatggc
     2401 agaatggttt caggcgggct attttactat gtctttattg gtgaagagag cgtgtgatga
     2461 aagcttccaa cctcttggcg atatcatgaa aatgtgggga agggttccct tttctccagg
     2521 tccagctccc cctcctcata tgggagagct ggaccaggaa cgactgacca ggcagcaaga
     2581 actcacagcc ttataccaga tgcagcacct gcagtaccag cagtttttaa tacaacaaca
     2641 atatgcacag gttttggccc aacagcagaa agcagcactg tcttcccagc agcagcagca
     2701 gttggcactt cttcttcaac agtttcagac cttgaagatg agaatatctg atcagaacat
     2761 cattccctca gtaactaggt ctgtgtccgt gccagatact ggctctatct gggagcttca
     2821 gccaacagct tcacagccta cagtttggga aggtggtagt gtatgggatc ttcctctgga
     2881 caccacgaca ccaggccctg ccctggaaca gcttcagcag ctagagaagg ccaaagctgc
     2941 aaagctagag caagagagaa gagaggcaga aatgagggca aaacgggaag aggaagagcg
     3001 aaagaggcag gaagaactcc gaagacaaca ggaggaaatt cttcggcgac agcaggaaga
     3061 agaaaggaaa aggcgagagg aagaagaact tgcccgaagg aaacaggaag aggctctgcg
     3121 tcgccagcgg gagcaagaaa ttgcattaag gcgacagcga gaagaggaag aaagacagca
     3181 gcaagaagaa gctcttagaa gactggaaga gaggagaaga gaagaggaag aaaggcggaa
     3241 gcaggaagaa ttgttacgca aacaggaaga ggaggctgca aaatgggccc gggaagaaga
     3301 agaagcccag cgtcgattag aggagaaccg gctgcggatg gaagaggagg cagccagact
     3361 ccggcatgag gaagaagaac ggaagagaaa ggagctggag gtccagcggc agaaggagtt
     3421 aatgcgccag aggcagcagc agcaagaggc tctccggagg ttgcagcagc agcagcagca
     3481 acaacagctg gcgcagatga agcttccttc ttcttcaacg tggggccagc agtccaatac
     3541 aacagcatgt cagtcccagg ccacgctgtc gttggctgaa atccaaaaac tagaggaaga
     3601 acgagaacgg cagcttcgag aagagcaaag gcgccagcag agggagttga tgaaagctct
     3661 tcagcagcag cagcagcagc aacagcagaa actctcaggt tgggggaatg tcagcaaacc
     3721 ttcaggtacc acgaaatctc ttctggagat ccagcaggaa gaggccaggc aaatgcaaaa
     3781 gcagcagcag cagcagcagc aacaccagca accaaacaga gctcgtaaca atacgcattc
     3841 caacctgcac accagcattg ggaattctgt ttggggctct ataaatactg gtcctcctaa
     3901 ccagtgggca tctgacctag tcagtagtat ttggagtaat gctgacacta aaaactccaa
     3961 catgggattc tgggatgatg cagtgaaaga ggtgggacct aggaattcaa caaataaaaa
     4021 taaaaacaac gccagtctca gtaaatctgt aggtgtgtct aaccggcaga ataagaaagt
     4081 agaagaagaa gaaaagttgc tgaagctctt tcagggagta aataaagccc aagatggatt
     4141 tacgcagtgg tgtgaacaga tgcttcatgc ccttaatacg gcaaataact tggatgttcc
     4201 cacatttgtt tctttcctga aagaagtaga atctccttat gaggtccatg attatatcag
     4261 ggcctattta ggagatactt ctgaggccaa ggagtttgcc aagcagttcc ttgagcgccg
     4321 tgccaaacag aaagccaacc agcagcgtca gcagcagcag ctgccgcagc agcagcagca
     4381 gccgccacag cagccgccac agcagccaca acagcaggac tctgtgtggg ggatgaacca
     4441 cagtacactc cattcagtat ttcagaccaa tcaaagcaac aaccaacaat ccaattttga
     4501 ggctgtgcag agtggcaaga agaagaaaaa gcagaagatg gtccgagcag atcccagttt
     4561 attaggattt tcagtcaatg catcatcgga gcgactcaac atgggtgaaa tcgagacgtt
     4621 ggatgactac tgaggatcca ccggatctag ataactgatc ataatcagcc ataccacatt
     4681 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     4741 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     4801 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4861 gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat
     4921 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     4981 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     5041 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     5101 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     5161 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     5221 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     5281 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     5341 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5401 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5461 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     5521 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5581 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5641 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5701 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5761 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5821 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
     5881 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     5941 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     6001 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     6061 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     6121 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     6181 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     6241 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     6301 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     6361 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     6421 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     6481 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     6541 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     6601 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     6661 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     6721 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     6781 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag
     6841 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     6901 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg
     6961 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     7021 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     7081 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga
     7141 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7201 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7261 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7321 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7381 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7441 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7501 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7561 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7621 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7681 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7741 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7801 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7861 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7921 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt
     7981 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccg
//
