LOCUS       sequence-38995-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  TITLE     Boussiotis Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: His-RIAM
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    96..258
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             271..288
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             292..324
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(328..336,337..351)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag ctggctagcg tttaaactta agcttagcgc agaggcttgg ggcagccgag
      121 cggcagccag gccccggccc gggcctcggt tccagaaggg agaggagccc gccaaggcgc
      181 gcaagagagc gggctgcctc gcagtccgag ccggagaggg agcgcgagcc gcgccggccc
      241 cggacggcct ccgaaaccat ggggggttct catcatcatc atcatcatgg tatggctagc
      301 atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggtaccagga
      361 tccagtgtgg tggaattcat gggtgagtca agtgaagaca tagaccaaat gttcagcact
      421 ttgctgggag agatggatct tctgactcag agtttaggag ttgacactct ccctcctcct
      481 gaccctaatc cacccagagc tgaatttaac tacagtgtgg ggtttaaaga tttaaatgag
      541 tccttaaatg cactggaaga ccaagattta gatgctctca tggcagatct ggtagcagac
      601 ataagtgagg ctgagcagag gacaatccag gcacagaaag agtccttgca gaatcaacat
      661 cattcagcat ct
//
