LOCUS       sequence-389953-        2392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the guide RNA targeting the sequence GGACAGTCCTGCCGAGGTGG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Stevenson ZC, Moerdyk-Schauwecker MJ, Banse SA, Patel DS, Lu H, 
            Phillips PC
  TITLE     High-throughput library transgenesis in Caenorhabditis elegans via 
            Transgenic Arrays Resulting in Diversity of Integrated Sequences 
            (TARDIS).
  JOURNAL   Elife. 2023 Jul 4;12:RP84831. doi: 10.7554/eLife.84831.
  PUBMED    37401921
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-07-4"; volume: "12:RP84831. doi"; pages: " 
            10.7554/eLife.84831"
COMMENT     Sequence Label: pMS84 (U6p::GGACAGTCCTGCCGAGGTGG)
FEATURES             Location/Qualifiers
     source          1..2392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(148..165)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(148..164)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        186..535
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     misc_RNA        556..631
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     rep_origin      complement(771..1359)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(851..870)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1530..2321,2322..2390))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2153..2172
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(2391..2392,1..103))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
       61 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
      121 tgccacatta tacatagttg ataattcact ggccgtcgtt ttacaacgtc gttactggga
      181 aaacccaaaa aaaactagca ataaaggaat aaaaaactgt acaccttaaa ggcgcacact
      241 ctgttttgca aattttattt ttagttgtga attttctgct gagacctgaa aatagcaact
      301 ttagtactac tataatttgt caaccttttc aaaaaaagca tgcaattttt gagaaactct
      361 tataaaagct attattaaaa aaacaccttt tttccaaaat tattccacaa aaaatatgtt
      421 atgaaatgcc tacaccctct cacacacact ctttatacta ctctgtcaaa ctcacgagat
      481 gtctgccgcc tcttgtgttg cccctatata aacacctcct attgcgagat gtcttggaca
      541 gtcctgccga ggtgggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
      601 tcaacttgaa aaagtggcac cgagtcggtg ctttttgtga aatttctggc gtaatagcga
      661 agaggcccgc accgaaacgc ccttcccaac agttgcgcag cctgaatggc gaattgagca
      721 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      781 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      841 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      901 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      961 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1021 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1081 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1141 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1201 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1261 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1321 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1381 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1441 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1501 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1561 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1621 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc
     1681 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1741 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1801 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1861 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1921 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1981 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2041 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2101 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2161 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2221 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2281 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2341 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat ac
//
