LOCUS       sequence-38997-e         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  TITLE     Boussiotis Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Smad3-shRNA
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     123..142
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     terminator      complement(358..452)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata
       61 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      121 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      181 tgtggaaagg acgaggatcc acctgagtga agatggagat tcaagagatc tccatcttca
      241 ctcaggtttt tttacgcgtg aattctctag accattcgtt tggcgcgcgg gcccagtagg
      301 taagtgaaca tggtcatagc tgtttcctag gagatcctgg tcatgactag tgcttggatt
      361 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
      421 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctctagttat taatagtaat
      481 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      541 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      601 atgttcccat ngtaacgcca atagggactt tccatgacgt ccatggcgtg cagtatttac
      661 ggtaaactgc
//
