LOCUS       sequence-389994-        2066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 module 5T with Esp3I expansion point.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pJMC-5T-Esp3I
FEATURES             Location/Qualifiers
     source          1..2066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        133..237
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1322..1910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1811..1830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2064..2066,1..15)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gagtcagtga gcgaggaagc ggaagagcgc ccacctgctt ttcctcaccg tgagacgaac
       61 aacaacgtct ctagtgtttt gcaggtgtga tgaaaagcct tagggtcagg tggcactttt
      121 cggggaaatg tgcgatgaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      181 ccgctaatga gacaataacc ctgataaatg cttcaattat attgaaaaag gaagagtatt
      241 gaaaaaggaa gagtatgagt atccaacatt tccgtgtcgc ccttattccc ttttttgcgg
      301 ccttctgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      361 atcagttggg tgcccgagtg ggttacattg aactggatct caacagcggc aaaatcttag
      421 agagttttcg ccccgaagaa cgcttcccaa tgatgagcac tttcaaagtt ctgctatgtg
      481 gcgcggtgtt atcccgtatt gatgctgggc aagagcaact cggtcgccgc atacactatt
      541 ctcagaatga cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga
      601 cagtaagaga attatgtagt gctgccataa ccatgagtga taacactgcg gccaacttac
      661 ttctgacaac gattggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
      721 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
      781 gtgacaccac gatgcctgta gcaatggcaa caactctccg caaactatta actggcgaac
      841 tacttactct tgcttcacgc cagcaactca ttgactggat ggaggcggac aaagttgcag
      901 gaccacttct gcgctcggca cttccggctg gctggtttat tgctgataaa tctggagccg
      961 gtgagcgtgg ctctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1021 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1081 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agacgaaggg aaataaatag
     1141 tagcccgcct gatgtgcggg cttttttttg gtggtggtta ccagtgtcgc ttagctaccg
     1201 taggtcctct taggcggtcc gattagcgac atagtcatca atgccttgac ggcaaaatcc
     1261 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1321 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1381 cagcggtggt ttgtttgccg gatcaagagc tactaactct ttttccgaag gtaactggct
     1441 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1501 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1561 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1621 aggcgcagcg gtcgggctga acggggggtt cgtgctcaca gcccagcttg gagcgaacga
     1681 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1741 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1801 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1861 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1921 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1981 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2041 gccgcagccg aacgaccgag cgcagc
//
