LOCUS       sequence-389996-       14347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  35S promoter-driven expression of the positive-sense TSWV M RNA 
            segment encoding CBE in place of viral GP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14347)
  AUTHORS   Liu Q, Zhao C, Sun K, Deng Y, Li Z
  TITLE     Engineered biocontainable RNA virus vectors for non-transgenic 
            genome editing across crop species and genotypes.
  JOURNAL   Mol Plant. 2023 Mar 6;16(3):616-631. doi: 
            10.1016/j.molp.2023.02.003. Epub 2023 Feb 7.
  PUBMED    36751129
REFERENCE   2  (bases 1 to 14347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molp.2023.02.003"; journalName: "Mol Plant"; date: 
            "2023-03-6- 6"; volume: "16"; issue: "3"; pages: "616-631"
COMMENT     Sequence Label: pM-CBE
FEATURES             Location/Qualifiers
     source          1..14347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        423..768
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     711..731
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             859..924
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             931..951
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1624..1647,1648..1650,1651..5724)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5746..5994
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6007..6027
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7416..7668
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7716..7740
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7868..9210
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(8831..8878)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9251..9269
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9307..9329)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9429..9448
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9469..9750
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             9751..10425
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(10528..10983)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10665..10686)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10877..10896
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(11099..12247)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12546..13340)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEANGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13549..14180
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    14260..14284
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
       61 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
      121 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
      181 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
      241 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag
      361 cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca
      421 attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct
      481 atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat
      541 tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga
      601 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
      661 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
      721 caagaccctt cctctatata aggaagttca tttcatttgg agaggagagc aatcagtgca
      781 aacaaaaacc ttaatccaga catcttgaaa ttaatcatat aaccattata atctgagcag
      841 acgtataaga ctagtatgga ttacaaggac cacgacggtg attataagga tcacgacatc
      901 gactacaagg acgatgatga caagatggct ccgaagaaga aaaggaaagt gggtatacga
      961 gaagcctctc cagcatctgg tcccaggcat ctgatggatc cacacatctt cacatccaac
     1021 ttcaacaacg gcatcggacg acacaagacg tatctgtgct acgaggtaga gaggttggac
     1081 aacgggactt cagtgaagat ggaccagcac agaggcttct tgcacaacca agccaagaac
     1141 ttgctgtgcg gattctacgg gaggcatgcc gagttaagat tcctcgactt ggttccaagc
     1201 ttgcagctcg acccggcaca gatctatcga gtcacgtggt tcatcagttg gtccccgtgc
     1261 ttcagttggg gatgtgcagg tgaggtgaga gcattcctgc aggagaacac tcatgtccga
     1321 ttgcgtatct tcgcagcccg aatctacgac tacgacccat tgtacaaaga ggcactgcag
     1381 atgctcaggg acgccggtgc acaggtctcc atcatgacat acgacgagtt caagcactgt
     1441 tgggacacat tcgtcgatca tcaaggttgc ccattccagc cttgggacgg tttggacgaa
     1501 cacagccagg ctctctctgg aaggctgagg gccattcttc agaaccaggg caactcagga
     1561 tctgaaacgc cgggtaccag tgaaagtgcc acacccgaga gtatacacgg tgttccagca
     1621 gccgataaga agtacagtat cggacttgcc atagggacta actctgtcgg gtgggcagta
     1681 atcacagatg agtacaaagt cccatctaag aagttcaagg tgctcggtaa caccgataga
     1741 cacagcatca agaagaacct catcggtgct ctcctgttcg attccggcga aacggccgaa
     1801 gccactaggt tgaagaggac ggcccgacga cgttataccc gtagaaagaa taggatatgc
     1861 tatctgcagg agatattctc caacgagatg gctaaggtag acgatagctt cttccatcgt
     1921 ctcgaggagt ccttcctggt ggaagaggac aagaaacatg aaagacaccc aatctttggg
     1981 aacattgttg acgaggtcgc atatcatgag aagtacccaa caatctacca cttgaggaag
     2041 aaactcgtcg acagtactga caaggctgat ctgcgtttaa tctacttagc actggctcat
     2101 atgatcaagt tcagaggtca cttcttgatc gagggtgact tgaaccctga caattcagac
     2161 gtagacaagc tgttcatcca gttagtccag acgtataatc agttgttcga ggaaaaccca
     2221 attaacgcta gtggagtcga cgccaaggca atattgagtg caagactctc caagtcccga
     2281 aggttggaaa acctcattgc tcaattgcct ggagagaaga agaacgggct cttcggaaat
     2341 cttatagctc tgtccttagg gttaacgccg aatttcaagt ccaacttcga cctcgccgag
     2401 gacgccaagt tgcaattgtc caaggatacc tacgacgatg acctcgacaa cttactggct
     2461 cagatcggcg accagtacgc tgacctcttc ttggccgcta agaacttgtc agacgcaatc
     2521 ctcttaagtg atatcttacg tgtgaacaca gagattacga aagctccttt gagcgcatcc
     2581 atgatcaaga gatacgatga gcaccatcag gatttgacgt tgttgaaggc cttagttcgt
     2641 cagcaattgc ccgagaagta caaggagatc ttcttcgatc agtccaagaa cggatacgca
     2701 gggtacatcg atggtggtgc tagtcaagag gagttttaca agttcatcaa gcccatactc
     2761 gagaagatgg atggaactga ggaactgtta gtgaagctga atcgtgagga cctgttaagg
     2821 aagcagcgaa cgttcgacaa tggcagcatc ccgcaccaga tccaccttgg cgagctgcat
     2881 gcaatcttga ggagacagga agacttctat ccattcttga aggacaacag agagaagatc
     2941 gagaagatct tgacgttcag aatcccgtat tacgttggtc cactcgccag gggtaactcc
     3001 cgtttcgctt ggatgacgag gaaatcagag gagacaatca ctccttggaa cttcgaggag
     3061 gtggttgaca aaggagccag cgctcaatcc ttcatagagc gtatgaccaa tttcgacaag
     3121 aatctcccaa acgagaaggt cctgccgaaa cactcattgt tgtacgagta cttcactgtg
     3181 tataacgagc tcacgaaggt caaatacgta actgagggta tgcgaaagcc cgccttcctc
     3241 tccggtgaac agaagaaggc aatcgtcgat ttgttgttca agaccaacag gaaggtcact
     3301 gtgaagcaac tgaaagagga ctacttcaag aagatagagt gcttcgattc tgtggagatc
     3361 agtggtgttg aggatcgttt caacgcatcc ttgggcacat accatgattt gttgaagatc
     3421 atcaaggaca aggacttcct cgacaacgag gagaacgagg acatcctcga ggatatcgtt
     3481 ctcacattga cgctcttcga ggatcgtgag atgatcgagg agagactgaa gacctacgct
     3541 catttgttcg atgataaggt gatgaagcaa ctcaagaggc gtcgatacac gggatgggga
     3601 aggttgtctc ggaagctgat caacggtatt agggataaac agtctggcaa gacaatcttg
     3661 gacttcttga agagtgatgg attcgcaaat aggaatttca tgcagttaat ccacgatgat
     3721 agtctgacgt tcaaggagga catccaaaag gcccaagtga gtggtcaggg agacagcctc
     3781 catgaacata tagcaaactt ggctggatcc ccagcaatta agaaaggaat cttgcagacg
     3841 gtcaaagttg tggatgaact tgttaaggtc atgggtcgtc acaaacctga gaatatcgtt
     3901 atcgaaatgg cccgagagaa tcagacgacc cagaaaggac agaagaactc tcgtgaacgt
     3961 atgaaacgaa tagaagaagg gatcaaagag ctcggtagcc agatcctgaa ggagcaccca
     4021 gtagagaaca ctcaactgca gaacgagaag ctgtatctgt actacttgca aaacgggcga
     4081 gatatgtatg ttgaccagga gctcgacatc aatagacttt ctgactacga tgttgaccat
     4141 attgtgccgc aaagcttctt gaaggatgat agcatagata acaaagttct cacacgttcc
     4201 gacaagaaca gaggtaagag cgacaacgtg ccgagtgaag aagtcgttaa gaagatgaag
     4261 aactactgga ggcagttgct caacgctaag ttgatcaccc agaggaagtt cgacaacctc
     4321 acaaaggctg agagaggagg cctgtcagag ttggataagg ctgggttcat caagaggcag
     4381 cttgtcgaga cacgacagat caccaagcac gtagcccaga tcctcgattc aaggatgaac
     4441 accaagtacg atgagaacga caagctcatc agggaagtga aggtaatcac gctgaagagc
     4501 aagctcgtat ccgacttccg aaaggacttt cagttctaca aggtgcgtga gatcaacaac
     4561 taccaccatg ctcatgatgc atatcttaat gcagtggttg ggacggctct cattaagaag
     4621 tacccaaagc tcgaatctga atttgtgtat ggcgattaca aggtttatga tgtccgtaag
     4681 atgattgcta agagcgagca ggagattggt aaggctaccg cgaagtactt cttctactct
     4741 aacatcatga acttcttcaa gacggagatc accctggcca acggagagat caggaagcgt
     4801 ccgctcatcg agaccaacgg tgagaccggt gagatcgtgt gggacaaggg acgtgacttc
     4861 gccactgtga ggaaggtgtt gagcatgccg caggtgaaca tcgtgaagaa gacggaggtg
     4921 cagaccggag ggttctccaa ggaatcaatc ttgccaaaga ggaactccga taaactcata
     4981 gcaaggaaga aggattggga tcccaagaag tatggagggt tcgactcccc gaccgtcgcc
     5041 tacagcgtct tggttgtggc aaaggtggag aagggaaaga gcaagaagtt gaaatcagtc
     5101 aaggagctcc ttggaatcac aatcatggag cgaagttctt tcgagaagaa ccccatagac
     5161 ttcttggagg ccaaaggtta caaagaagtg aagaaagact tgataatcaa gttgcctaag
     5221 tactccttgt tcgagctcga gaacgggagg aagcgaatgt tggccagcgc cggagaactt
     5281 cagaaaggca acgagctcgc tctcccaagc aagtacgtga atttcttgta cctcgcatca
     5341 cactacgaga agctgaaggg cagcccggag gataacgaac agaagcagct cttcgtggag
     5401 caacacaagc actacttgga tgagatcatc gagcagatta gcgagttcag caagagggta
     5461 atcttggctg acgctaatct ggacaaggtt ctcagcgcat acaacaagca tcgtgacaaa
     5521 cccatccgag agcaggcaga gaacatcatc cacttgttca ccctgactaa tttgggagca
     5581 cccgcagcct tcaagtactt cgatactacc atcgaccgga agagatacac aagtaccaag
     5641 gaggtcctgg atgcaacctt gatccatcag tccatcaccg gcttgtacga gactaggatc
     5701 gacctctctc agttgggtgg agacactaga gactccggag gcagcacaaa cctcagcgac
     5761 atcatcgaga aggagacagg caagcagttg gtcatccagg agtctatcct catgctgccc
     5821 gaggaggttg aggaggtcat tggcaataag cctgagagcg acatacttgt gcacaccgca
     5881 tatgacgaat ccacagatga gaatgtgatg cttctgacat cagatgcacc tgagtacaag
     5941 ccatgggccc tcgtgatcca ggactcaaac ggtgagaaca agatcaagat gttgagcggc
     6001 gggtcgccta agaagaagcg taaagtgtaa cgcgtcgacg gctgcttgtc acaagctaaa
     6061 tttgatccta tttcctacat taatcgaatt atatatatat aaaataaaca aaaaacaaac
     6121 aaaaatacaa ataaacaaaa aacaacaaaa agagtcttcg gaccaaagtt tggcttttca
     6181 gccttatttt gtttttgttt tttgtttttg attttttgtt tgttttttat ttttgttttt
     6241 gtttggtttt tgatttaaaa atgcaaaata aacagagatt taagcttaaa taagtggtat
     6301 taaaagtact atatttcatc aaaagataac tgagcaacat tatcagaaac cccttcctcc
     6361 tcttcttcaa ctgatctctc aagatttgag ctcagttctt taagctgttt tttaatctgc
     6421 ttctcactgt ttcctttagg aattatcagc ttgcaagcct caatgaatgc ttgagatcta
     6481 gctctaatag ccctgtttag aggtatgacc atgcaacttt tgtctttatc agctctgggt
     6541 gaatcacaaa actcctttgt ccaagaatac atgacactac caaaagaaac ccccttcttg
     6601 tattcttgac tgcacatcaa gtgcagctga cagcagtttt ctggagtgtt attcattttc
     6661 ggaatagacc agttcagata aaaaacaaaa cagataggat cagttattgt cccctgaccc
     6721 ttcagaatga tttgcttttc agatggcatg ttgggatcga ccagagcaac cacaagtttc
     6781 cctgtagggt ttggtatagt ggggcagacc catatcacaa tcctggaaat catcatgtat
     6841 tgttttctgc tgtcccaagt tggacagatc ttgatgacct tattagcgtt ttgcttcccg
     6901 ttgccgacaa aaagatcatt tttccagttt gagatatggt ggtttgtatc tactatcatt
     6961 cttgcagaaa gatcataacc ctctgattct gtgatagaat cagattcata tgttccaaag
     7021 gaagatgttc cctctgtgtt caacagtatc tttcctttgg atgtatccat agctttggtt
     7081 aaagcaagct tttcatcaga agaagaccat ggttttgaga cttcaacatt accgttatgt
     7141 ttagcaagtg aaactaaagg cccttcatcc tttctggcag acttagaaga ccccttatta
     7201 ccaaaaaaag tcaacatttt gagttcaaca gcctgaggta gaggagcttg tgtaatgtaa
     7261 ttaattgatt cttagcaaag tgtataatag gtatatttct gatgcactga ttgctctggg
     7321 tcggcatggc atctccacct cctcgcggtc cgacctgggc atccgaagga ggacgtcgtc
     7381 cactcggatg gctaagggag agctcgaatt tccccgatcg ttcaaacatt tggcaataaa
     7441 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     7501 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     7561 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     7621 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg aattcagatt
     7681 gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
     7741 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
     7801 tccgttcgtc catttgtatg tgcatgccaa ccacaggaga tctcagtaaa gcgaaaagtg
     7861 ccacctgaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct
     7921 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc
     7981 gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga
     8041 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
     8101 agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc
     8161 cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc
     8221 tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct
     8281 actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg
     8341 attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta
     8401 taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat
     8461 tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat
     8521 gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt
     8581 gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag
     8641 ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc
     8701 aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt
     8761 tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca
     8821 aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa
     8881 agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac
     8941 tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg
     9001 catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag
     9061 gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc
     9121 agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt
     9181 caatgctatc atttcctttg atattggatc atactaagaa accattatta tcatgacatt
     9241 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     9301 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     9361 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     9421 taactatgcg gcatcagagc agattgtact gagagtgcac catagatcaa cgacattact
     9481 atatatataa tataggaagc atttaataga acagcatcgt aatatatgtg tactttgcag
     9541 ttatgacgcc agatggcagt agtggaagat attctttatt gaaaaatagc ttgtcacctt
     9601 acgtacaatc ttgatccgga gcttttcttt ttttgccgat taaaaattaa ttcggtcgaa
     9661 aaaagaaaag gagagggcca agagggaggg cattggtgac tattgagcac gtgagtatac
     9721 gtgattaagc acacaaaggc agcttggagt atgtctgtta ttaatttcac aggtagttct
     9781 ggtccattgg tgaaagtttg cggcttgcag agcacagagg ccgcagaatg tgctctagat
     9841 tccgatgctg acttgctggg tattatatgt gtgcccaata gaaagagaac aattgacccg
     9901 gttattgcaa ggaaaatttc aagtcttgta aaagcatata aaaatagttc aggcactccg
     9961 aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc tctggtcaat
    10021 gattacggca ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga ataccaagag
    10081 ttcctcggtt tgccagttat taaaagactc gtatttccaa aagactgcaa catactactc
    10141 agtgcagctt cacagaaacc tcattcgttt attcccttgt ttgattcaga agcaggtggg
    10201 acaggtgaac ttttggattg gaactcgatt tctgactggg ttggaaggca agagagcccc
    10261 gaaagcttac attttatgtt agctggtgga ctgacgccag aaaatgttgg tgatgcgctt
    10321 agattaaatg gcgttattgg tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa
    10381 gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga aataggttat tactgagtag
    10441 tatttattta agtattgttt gtgcacttgc cgatctatgc ggtgtgaaat accgcacaga
    10501 tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg
    10561 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
    10621 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
    10681 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
    10741 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
    10801 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
    10861 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
    10921 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
    10981 cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    11041 gctattacgc cagatccttg ctggctgaac ccccagccgg aactgacccc acaaggccct
    11101 agcgtttgca atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc
    11161 gcaactcttc gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg
    11221 atccgcacat gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga
    11281 aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt
    11341 tcgtgtagtg gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac
    11401 gggacgtttt cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca
    11461 ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt
    11521 cctcggcctt cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt
    11581 agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct
    11641 gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt
    11701 ccttgttgac gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc
    11761 gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc
    11821 acggcgcaat atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca
    11881 gcaacgcggc ctgcttggcc tcgctgacct gttttgccag gtcctcgccg gcggtttttc
    11941 gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg
    12001 cctcctgttc gagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg
    12061 gagccagttg cacgctgtcg cgctcgatct tggccgtagc ttgctgggcc atcgagccga
    12121 cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat
    12181 cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc
    12241 gattcattca ccctccttgc gggattgccc cgactcacgc cggggcaatg tgcccttatt
    12301 cctgatttga cccgcctggt gccttggtgt ccagataatc caccttatcg gcaatgaagt
    12361 cggtcccgta gaccgtctgg ccgtccttct cgtacttggt attccgaatc ttgccctgca
    12421 cgaataccag cgaccccttg cccaaatact tgccgtgggc cttggcctga gagccaaaac
    12481 acttgatgcg gaagaagtcg gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct
    12541 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
    12601 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
    12661 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
    12721 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
    12781 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
    12841 tctttaaatg gggtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
    12901 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
    12961 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
    13021 ctttgttcat cttcatactc ttccgagcaa aggacgccat tggcctcact catgagcaga
    13081 ttgctccagc catcatgccg ttcaaggtgc aggacctttg gaacaggcag ctttccttcc
    13141 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
    13201 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
    13261 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
    13321 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
    13381 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaact
    13441 ttaaatacca gaaaacagct tttttaaagt tgttttcaaa gttggcgtat aacatagtat
    13501 cgacggagcc gattttgaaa ccacaattat gggtgatgct gcaactcgag cgggccggga
    13561 gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc
    13621 cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag acaggttagc
    13681 ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct gtggacagcc
    13741 cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca agtgtcaagg
    13801 atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc agggcactta
    13861 tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg ttttcgccga
    13921 tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca
    13981 acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga
    14041 gggccaagtt ttccgcgagg tattcacaac gccggcggcc gcggtgtctc gcacacggct
    14101 tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca gcggcgaggg
    14161 caaccagccc ggtgagcgtc tctagtggac tgatgggctg cctgtatcga gtggtgattt
    14221 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
    14281 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg
    14341 tggtttt
//
