LOCUS       sequence-39000-e         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  AUTHORS   White MD, Milne RV, Nolan MF
  TITLE     A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or 
            Down-Regulate Neuronal Gene Expression in vivo.
  JOURNAL   Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4.
  PUBMED    21772812
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2011"; volume: "4"; pages: "8. Epub 2011 Jul 4"
COMMENT     Sequence Label: pENTR-L5-Kir2.1-mCherry-L2
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..30
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    61..156
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      156..165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgactcacta tagggcgaat tgaaggaagg ccgtcaaggc ctaggcgcgc ctcgggcccc
       61 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat
      121 gcttttttat aatgccaact ttgtatacaa aagttgccgc catgggcagt gtgagaacca
      181 accgctacag catcgtctct tcggaggaag atggcatgaa gctggccact atggcagttg
      241 ccaatggctt tgggaatggc aagagtaaag tccatacccg acaacagtgc aggagccgct
      301 ttgtgaagaa agatggtcat tgcaatgttc agtttatcaa cgtgggtgag aagggacaga
      361 ggtacctggc agacatcttt actacctgtg tcgacatccg ctggaggtgg atgctggtta
      421 tcttctgtct tgccttcgtg ctctcctggc tgttctttgg ctgtgtgttt tggttgatag
      481 ccctgctcca tggggatcta gatacttcta aagtgagcaa agcatgcgtg tcagaggtca
      541 acagcttcac ggctgccttc ctcttctcca tcgagaccca gacaaccatt ggctatggtt
      601 tcaggtgtgt gacagacgag tgcccaattg ctgtcttcat ggtggtattc c
//
