LOCUS       sequence-390009-        4358 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of eGFP-Rab31 (human) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Banworth MJ, Liang Z, Li G
  TITLE     A novel membrane targeting domain mediates the endosomal or Golgi 
            localization specificity of small GTPases Rab22 and Rab31.
  JOURNAL   J Biol Chem. 2022 Sep;298(9):102281. doi: 10.1016/j.jbc.2022.102281.
            Epub 2022 Jul 19.
  PUBMED    35863437
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-09"; volume: "298(9):102281. doi"; pages: " 
            10.1016/j.jbc.2022.102281. Epub 2022 Jul 19"
COMMENT     Sequence Label: pBI-eGFP-Rab31
FEATURES             Location/Qualifiers
     source          1..4358
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(136..154,155..177,178..196,197..219,220..238,239..261,
                     262..280,281..303,304..322,323..345,346..364,365..387,
                     388..406)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        439..476
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          658..1230
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(1362..1381)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1481..1500)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1873..1890)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2044..2632)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2124..2143)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2803..3594,3595..3663))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3426..3445
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3664..3768)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3836..3854
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    4199..4333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4236..4255)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4290..4309
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctgcaggaat tcggggccgc ggaggctgga tcggtcccgg tgtcttctat ggaggtcaaa
       61 acagcgtgga tggcgtctcc aggcgatctg acggttcact aaacgagctc tgcttatata
      121 ggtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
      181 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
      241 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
      301 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      361 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      421 gctcggtacc cgggtcgagt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
      481 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      541 accgggaccg atccagcctc cgcggccccg aattcgagct cggtacccgg ggatcctcta
      601 gtcagctgac gcgtgctagc gcggcctcga cgatatctct agactgagaa cttcagggtg
      661 agtttgggga cccttgattg ttctttcttt ttcgctattg aaaaattcat gttatatgga
      721 gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga
      781 ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt
      841 attttctttt cattttctgt aacttttttc gttaaacttt agcttgcatt tgtaacgaat
      901 ttttaaattc actttcgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt
      961 tcaaggcaat cagggtaatt atattgtact tcagcacagt tttagagaac aattgttata
     1021 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt
     1081 attggtagaa acaactacat cctggtaatc atcctgcctt tctctttatg gttacaatga
     1141 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac
     1201 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tgttgtgctg
     1261 tctcatcatt ttggcaaaga attcactcct caggtgcagg ctgcctatca gaaggtggtg
     1321 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     1381 aattatgggg acatcatgaa gccccttgag catctgactt ctgggtaata aaggaaattt
     1441 attttcattg caatagtgtg tgggaatttt ttgtgtctct cactcggaag gacatatggg
     1501 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     1561 atatgctggc tgccatgaac aaaggtggct ataaagaggt catcagtata tgaaacagcc
     1621 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     1681 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     1741 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     1801 aactcgactg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1861 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1921 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1981 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2041 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2101 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2161 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2221 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2281 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2341 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2401 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2461 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     2521 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2581 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2641 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2701 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2761 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2821 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2881 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2941 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3001 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3061 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3121 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3181 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3241 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3301 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3361 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3421 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3481 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3541 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3601 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3661 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3721 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3781 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     3841 gcgtatcacg aggccctttc gtcttcactc gacgctggtc gagctgatac ttcccgtccg
     3901 ccaggggaca tgccggcgat gctgaaggtc gcgcgcattc ccgatgaaga ggccggttac
     3961 cgcctgttga cctggtggga cgggcagggc gccgcccgag tcttgcctcg gcggcgggcg
     4021 ctctgctcat ggagcgcgcg tccggggacc ttgcacagat agcgtggtcc ggccagacga
     4081 cgaggcttgc aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     4141 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     4201 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4261 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4321 tcatgtctgg atcctctaga actaggtcga cgcggccg
//
