LOCUS       sequence-39002-e         607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 607)
  AUTHORS   White MD, Milne RV, Nolan MF
  TITLE     A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or 
            Down-Regulate Neuronal Gene Expression in vivo.
  JOURNAL   Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4.
  PUBMED    21772812
REFERENCE   2  (bases 1 to 607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2011"; volume: "4"; pages: "8. Epub 2011 Jul 4"
COMMENT     Sequence Label: pENTR-R4r-LucmiR-EGFP-R3r
FEATURES             Location/Qualifiers
     source          1..607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(35..158)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           299..325
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           389..430
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     505..524
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      556..565
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctacaggtca ctaataccat ctaagtagtt gattcatagt gactggatat gttgtgtttt
       61 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
      121 attttacgtt tctcgttcaa cttttctata caaagttgct aaggtagcct tgcagaagtt
      181 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
      241 agaaactggg cttgtcgaga cagagaagac tcttgcgttt cggtcgactt ctagagctct
      301 ggaggcttgc tgaaggctgt atgctgattt gtattcagcc catatcggtt ttggccactg
      361 actgaccgat atggtgaata caaatcagga cacaaggcct gttactagca ctcacatgga
      421 acaaatggcc gaattcggcg ctagctgctg atatcgcata cgcgtggacc agataggcac
      481 ctattggtct tactgacatc cactttgcct ttctctccac aggtgtcggt accgcgggcc
      541 cgggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
      601 cccatcc
//
