LOCUS       sequence-390021-       11198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of ULK4 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11198)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-ULK4-V5-miniTurbo-STOP_IDG-K
FEATURES             Location/Qualifiers
     source          1..11198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4168..4188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4188..5399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5236..5255
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5400..5424
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5427..5436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(7177..7201)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7203..7244
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7251..8021
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(8027..8047)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    8076..8664
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8129..8149)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8547..8558)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8678..9177
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8713..8736)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9110..9129
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9198..9797
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9198..9217)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9694..9714
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9935..10168
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10240..10361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10277..10296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10331..10350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10401..10536
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10556..10575)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10557..10575)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10585..10602)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10585..10601)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10594..10616)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10743..11171
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10830..10849)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11040..11061
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggt ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagaca
     5401 caagtttgta caaaaaagca ggcttagcca ccatggaaaa ctttattctg tatgaggaga
     5461 tcggaagagg aagcaagact gttgtctata aagggcgacg gaagggaaca atcaattttg
     5521 tagccattct ttgtactgat aagtgcaaaa ggcctgaaat aaccaactgg gtccgtctca
     5581 cccgtgaaat aaaacacaag aatattgtaa cttttcatga atggtatgaa acaagcaacc
     5641 acctctggct agtggtggaa ctctgcacag gtggttcctt aaaaacagtt attgctcaag
     5701 atgaaaacct cccagaagat gttgtgagag aatttggaat tgacctgatt agtggattac
     5761 atcatcttca taaacttggc attctctttt gtgacatttc tcctaggaag atactcttgg
     5821 aagggcctgg cacactgaag tttagcaact tttgcttggc aaaagtggaa ggtgaaaatt
     5881 tggaagagtt ctttgctttg gtggcagcag aggaaggagg aggtgataat ggggaaaatg
     5941 tcctgaagaa aagcatgaaa agtagagtca aaggatctcc tgtatataca gcaccagaag
     6001 ttgtgagggg tgctgacttt tccatctcca gtgacctctg gtctttgggc tgtctgcttt
     6061 atgaaatgtt ttcaggaaaa cctccattct tctcagaaag tatttcagaa ttaactgaaa
     6121 agatcttatg tgaagatcct ttgccaccta ttccgaaaga ttcttctcgt cctaaagctt
     6181 cttcagattt tattaatttg cttgatgggt tacttcaaag agatcctcag aaaagattga
     6241 cttggacaag gctactgcag cattcatttt ggaagaaagc ttttgctgga gcagatcagg
     6301 aatcaagcgt cgaagatctc agtctcagca gaaacactat ggagtgttct gggccacaag
     6361 attccaagga gcttttgcag aactctcaga gtagacaagc aaaagggcac aagagtggtc
     6421 aaccactagg tcactctttc agactagaaa atccaactga gtttcggcct aagagtactc
     6481 ttgagggtca attgaatgaa tccatgtttc ttctcagttc tcgtcctact cccagaacta
     6541 gcactgcagt ggaagtaagt cctggtgagg atatgactca ctgttcacca cagaagactt
     6601 ctcctctgac caagattaca agtggacacc tgagtcagca ggacctggaa tcccagatga
     6661 gagagcttat ctacacggac tcagatcttg ttgtcacccc cattatcgac aatccaaaga
     6721 taatgaaaca gccaccagtt aaatttgatg caaaaatatt gcatctacca acatattcag
     6781 tggataagtt attatttctg aaagatcaag attggaatga ctttttgcaa caagtgtgct
     6841 cgcagatcga ctccactgag aagagcatgg gggcctcccg agccaagctg aatctccttt
     6901 gctatttgtg cgtggtggct ggtcaccagg aggtggccac caggctcctc cattcccccc
     6961 tgttccaatt gctaatccag catttgcgga tagctccaaa ctgggatata cgggccaagg
     7021 ttgctcacgt aattggttta ctggcttcgc acacagctga gctccaggaa aatacacctg
     7081 ttgttgagac tacaagctcc attggaatcg ggattttgaa ctgtcttgtt caacactcca
     7141 ctccagtgcc tagacagtgc cttgtgtatg tatcaaaccc agctttcttg tacaaagtgg
     7201 gcggcaagcc catccccaac cccctgctgg gcctggacag caccgctagc atcccgctgc
     7261 tgaacgctaa acagattctg ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg
     7321 tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt ggggatgctt
     7381 gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg ttctctcctt
     7441 ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg
     7501 gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg ggagcagaca
     7561 aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg gcaggcatcc
     7621 tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc gccgggatta
     7681 acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc acactgcagg
     7741 aagcagggat taacctggac aggaatactc tggccgctat gctgatccga gagctgcggg
     7801 cagccctgga actgttcgag caggaaggcc tggctccata tctgtcacgg tgggagaagc
     7861 tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag attttcggga
     7921 ttagccgggg gattgataaa cagggagccc tgctgctgga acaggacgga gttatcaaac
     7981 cctggatggg cggagaaatc agtctgcggt ctgccgaaaa gtaagccaac tttattatac
     8041 atagttgata tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt
     8101 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     8161 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     8221 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     8281 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     8341 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     8401 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     8461 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     8521 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     8581 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     8641 tctccctttg ggccgcctcc ccgcctggaa ttctaccggg taggggaggc gcttttccca
     8701 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     8761 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     8821 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     8881 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     8941 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     9001 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     9061 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     9121 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     9181 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     9241 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     9301 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     9361 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     9421 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     9481 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     9541 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     9601 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     9661 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     9721 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     9781 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
     9841 gaccccatgc atctcgaggg cccggtacct ttaagaccaa tgacttacaa ggcagctgta
     9901 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctagctca ctcccaacga
     9961 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
    10021 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    10081 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10141 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
    10201 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
    10261 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    10321 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
    10381 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
    10441 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
    10501 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
    10561 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
    10621 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
    10681 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
    10741 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
    10801 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
    10861 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
    10921 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    10981 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    11041 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
    11101 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
    11161 taacgcgaat tttaacaaaa tattaacgct tacaattt
//
