LOCUS       sequence-390025-       14092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  35S promoter-driven expression of the positive-sense TSWV M RNA 
            segment encoding ABE in place of viral GP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14092)
  AUTHORS   Liu Q, Zhao C, Sun K, Deng Y, Li Z
  TITLE     Engineered biocontainable RNA virus vectors for non-transgenic 
            genome editing across crop species and genotypes.
  JOURNAL   Mol Plant. 2023 Mar 6;16(3):616-631. doi: 
            10.1016/j.molp.2023.02.003. Epub 2023 Feb 7.
  PUBMED    36751129
REFERENCE   2  (bases 1 to 14092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molp.2023.02.003"; journalName: "Mol Plant"; date: 
            "2023-03-6- 6"; volume: "16"; issue: "3"; pages: "616-631"
COMMENT     Sequence Label: pM-ABE
FEATURES             Location/Qualifiers
     source          1..14092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        423..768
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     711..731
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             859..924
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             931..951
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5680..5700
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5713..5733
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5746..5766
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7161..7413
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7461..7485
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7613..8955
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(8576..8623)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     8996..9014
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9052..9074)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9174..9193
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9214..9495
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             9496..10170
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(10273..10728)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10410..10431)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10622..10641
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(10844..11992)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12291..13085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13294..13925
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    14005..14029
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
       61 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
      121 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
      181 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
      241 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag
      361 cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca
      421 attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct
      481 atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat
      541 tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga
      601 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
      661 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
      721 caagaccctt cctctatata aggaagttca tttcatttgg agaggagagc aatcagtgca
      781 aacaaaaacc ttaatccaga catcttgaaa ttaatcatat aaccattata atctgagcag
      841 acgtataaga ctagtatgga ttacaaggac cacgacggtg attataagga tcacgacatc
      901 gactacaagg acgatgatga caagatggct ccgaagaaga aaaggaaagt gtctgaagta
      961 gagttctccc acgagtactg gatgaggcac gctcttacat tggccaagcg tgctagggac
     1021 gagagagagg tgcctgtcgg agctgtgctt gtactgaaca accgagtaat cggcgaaggc
     1081 tggaaccgtg ccattggtct gcatgatccc accgcacacg cagagatcat ggcacttcgt
     1141 caaggaggtc ttgtaatgca gaactaccgt ctgatagatg caactctcta cgtgaccttc
     1201 gagccctgtg tcatgtgtgc tggagcaatg atccactccc gtatcggtcg agtggtattc
     1261 ggctggcgta acagcaagag aggtgctgct ggtagcctca tgaacgtgct gaactaccct
     1321 ggtatgaacc atcgtgtaga gatcactgag ggcattcttg cagacgagtg tgctgcactg
     1381 ctctgcgact tctacaggat gcctaggcag gtgttcaatg ctcagaagaa ggctcagagc
     1441 tccatcaata gcggtggatc ctcaggtgga tcttcaggca gtgagacacc tggaaccagt
     1501 gagtcagcta ctccagagtc aagtggtgga tcttcaggag gctcaataca cggtgttcca
     1561 gcagccgata agaagtacag tatcggactt gccataggga ctaactctgt cgggtgggca
     1621 gtaatcacag atgagtacaa agtcccatct aagaagttca aggtgctcgg taacaccgat
     1681 agacacagca tcaagaagaa cctcatcggt gctctcctgt tcgattccgg cgaaacggcc
     1741 gaagccacta ggttgaagag gacggcccga cgacgttata cccgtagaaa gaataggata
     1801 tgctatctgc aggagatatt ctccaacgag atggctaagg tagacgatag cttcttccat
     1861 cgtctcgagg agtccttcct ggtggaagag gacaagaaac atgaaagaca cccaatcttt
     1921 gggaacattg ttgacgaggt cgcatatcat gagaagtacc caacaatcta ccacttgagg
     1981 aagaaactcg tcgacagtac tgacaaggct gatctgcgtt taatctactt agcactggct
     2041 catatgatca agttcagagg tcacttcttg atcgagggtg acttgaaccc tgacaattca
     2101 gacgtagaca agctgttcat ccagttagtc cagacgtata atcagttgtt cgaggaaaac
     2161 ccaattaacg ctagtggagt cgacgccaag gcaatattga gtgcaagact ctccaagtcc
     2221 cgaaggttgg aaaacctcat tgctcaattg cctggagaga agaagaacgg gctcttcgga
     2281 aatcttatag ctctgtcctt agggttaacg ccgaatttca agtccaactt cgacctcgcc
     2341 gaggacgcca agttgcaatt gtccaaggat acctacgacg atgacctcga caacttactg
     2401 gctcagatcg gcgaccagta cgctgacctc ttcttggccg ctaagaactt gtcagacgca
     2461 atcctcttaa gtgatatctt acgtgtgaac acagagatta cgaaagctcc tttgagcgca
     2521 tccatgatca agagatacga tgagcaccat caggatttga cgttgttgaa ggccttagtt
     2581 cgtcagcaat tgcccgagaa gtacaaggag atcttcttcg atcagtccaa gaacggatac
     2641 gcagggtaca tcgatggtgg tgctagtcaa gaggagtttt acaagttcat caagcccata
     2701 ctcgagaaga tggatggaac tgaggaactg ttagtgaagc tgaatcgtga ggacctgtta
     2761 aggaagcagc gaacgttcga caatggcagc atcccgcacc agatccacct tggcgagctg
     2821 catgcaatct tgaggagaca ggaagacttc tatccattct tgaaggacaa cagagagaag
     2881 atcgagaaga tcttgacgtt cagaatcccg tattacgttg gtccactcgc caggggtaac
     2941 tcccgtttcg cttggatgac gaggaaatca gaggagacaa tcactccttg gaacttcgag
     3001 gaggtggttg acaaaggagc cagcgctcaa tccttcatag agcgtatgac caatttcgac
     3061 aagaatctcc caaacgagaa ggtcctgccg aaacactcat tgttgtacga gtacttcact
     3121 gtgtataacg agctcacgaa ggtcaaatac gtaactgagg gtatgcgaaa gcccgccttc
     3181 ctctccggtg aacagaagaa ggcaatcgtc gatttgttgt tcaagaccaa caggaaggtc
     3241 actgtgaagc aactgaaaga ggactacttc aagaagatag agtgcttcga ttctgtggag
     3301 atcagtggtg ttgaggatcg tttcaacgca tccttgggca cataccatga tttgttgaag
     3361 atcatcaagg acaaggactt cctcgacaac gaggagaacg aggacatcct cgaggatatc
     3421 gttctcacat tgacgctctt cgaggatcgt gagatgatcg aggagagact gaagacctac
     3481 gctcatttgt tcgatgataa ggtgatgaag caactcaaga ggcgtcgata cacgggatgg
     3541 ggaaggttgt ctcggaagct gatcaacggt attagggata aacagtctgg caagacaatc
     3601 ttggacttct tgaagagtga tggattcgca aataggaatt tcatgcagtt aatccacgat
     3661 gatagtctga cgttcaagga ggacatccaa aaggcccaag tgagtggtca gggagacagc
     3721 ctccatgaac atatagcaaa cttggctgga tccccagcaa ttaagaaagg aatcttgcag
     3781 acggtcaaag ttgtggatga acttgttaag gtcatgggtc gtcacaaacc tgagaatatc
     3841 gttatcgaaa tggcccgaga gaatcagacg acccagaaag gacagaagaa ctctcgtgaa
     3901 cgtatgaaac gaatagaaga agggatcaaa gagctcggta gccagatcct gaaggagcac
     3961 ccagtagaga acactcaact gcagaacgag aagctgtatc tgtactactt gcaaaacggg
     4021 cgagatatgt atgttgacca ggagctcgac atcaatagac tttctgacta cgatgttgac
     4081 catattgtgc cgcaaagctt cttgaaggat gatagcatag ataacaaagt tctcacacgt
     4141 tccgacaaga acagaggtaa gagcgacaac gtgccgagtg aagaagtcgt taagaagatg
     4201 aagaactact ggaggcagtt gctcaacgct aagttgatca cccagaggaa gttcgacaac
     4261 ctcacaaagg ctgagagagg aggcctgtca gagttggata aggctgggtt catcaagagg
     4321 cagcttgtcg agacacgaca gatcaccaag cacgtagccc agatcctcga ttcaaggatg
     4381 aacaccaagt acgatgagaa cgacaagctc atcagggaag tgaaggtaat cacgctgaag
     4441 agcaagctcg tatccgactt ccgaaaggac tttcagttct acaaggtgcg tgagatcaac
     4501 aactaccacc atgctcatga tgcatatctt aatgcagtgg ttgggacggc tctcattaag
     4561 aagtacccaa agctcgaatc tgaatttgtg tatggcgatt acaaggttta tgatgtccgt
     4621 aagatgattg ctaagagcga gcaggagatt ggtaaggcta ccgcgaagta cttcttctac
     4681 tctaacatca tgaacttctt caagacggag atcaccctgg ccaacggaga gatcaggaag
     4741 cgtccgctca tcgagaccaa cggtgagacc agtgagatcg tgtgggacaa gggacgtgac
     4801 ttcgccactg tgaggaaggt gttgagcatg ccgcaggtga acatcgtgaa gaagacggag
     4861 gtgcagaccg gagggttctc caaggaatca atcttgccaa agaggaactc cgataaactc
     4921 atagcaagga agaaggattg ggatcccaag aagtatggag ggttcgactc cccgaccgtc
     4981 gcctacagcg tcttggttgt ggcaaaggtg gagaagggaa agagcaagaa gttgaaatca
     5041 gtcaaggagc tccttggaat cacaatcatg gagcgaagtt ctttcgagaa gaaccccata
     5101 gacttcttgg aggccaaagg ttacaaagaa gtgaagaaag acttgataat caagttgcct
     5161 aagtactcct tgttcgagct cgagaacggg aggaagcgaa tgttggccag cgccggagaa
     5221 cttcagaaag gcaacgagct cgctctccca agcaagtacg tgaatttctt gtacctcgca
     5281 tcacactacg agaagctgaa gggcagcccg gaggataacg aacagaagca gctcttcgtg
     5341 gagcaacaca agcactactt ggatgagatc atcgagcaga ttagcgagtt cagcaagagg
     5401 gtaatcttgg ctgacgctaa tctggacaag gttctcagcg catacaacaa gcatcgtgac
     5461 aaacccatcc gagagcaggc agagaacatc atccacttgt tcaccctgac taatttggga
     5521 gcacccgcag ccttcaagta cttcgatact accatcgacc ggaagagata cacaagtacc
     5581 aaggaggtcc tggatgcaac cttgatccat cagtccatca ccggcttgta cgagactagg
     5641 atcgacctct ctcagttggg tggagactcc ggcggcagcc caaagaagaa gcggaaggtg
     5701 tctggaggtt ctcctaagaa aaagagaaaa gtgtccggcg gctccccgaa gaagaagcgc
     5761 aaggtgtagg agctacgcgt cgacggctgc ttgtcacaag ctaaatttga tcctatttcc
     5821 tacattaatc gaattatata tatataaaat aaacaaaaaa caaacaaaaa tacaaataaa
     5881 caaaaaacaa caaaaagagt cttcggacca aagtttggct tttcagcctt attttgtttt
     5941 tgttttttgt ttttgatttt ttgtttgttt tttatttttg tttttgtttg gtttttgatt
     6001 taaaaatgca aaataaacag agatttaagc ttaaataagt ggtattaaaa gtactatatt
     6061 tcatcaaaag ataactgagc aacattatca gaaacccctt cctcctcttc ttcaactgat
     6121 ctctcaagat ttgagctcag ttctttaagc tgttttttaa tctgcttctc actgtttcct
     6181 ttaggaatta tcagcttgca agcctcaatg aatgcttgag atctagctct aatagccctg
     6241 tttagaggta tgaccatgca acttttgtct ttatcagctc tgggtgaatc acaaaactcc
     6301 tttgtccaag aatacatgac actaccaaaa gaaaccccct tcttgtattc ttgactgcac
     6361 atcaagtgca gctgacagca gttttctgga gtgttattca ttttcggaat agaccagttc
     6421 agataaaaaa caaaacagat aggatcagtt attgtcccct gacccttcag aatgatttgc
     6481 ttttcagatg gcatgttggg atcgaccaga gcaaccacaa gtttccctgt agggtttggt
     6541 atagtggggc agacccatat cacaatcctg gaaatcatca tgtattgttt tctgctgtcc
     6601 caagttggac agatcttgat gaccttatta gcgttttgct tcccgttgcc gacaaaaaga
     6661 tcatttttcc agtttgagat atggtggttt gtatctacta tcattcttgc agaaagatca
     6721 taaccctctg attctgtgat agaatcagat tcatatgttc caaaggaaga tgttccctct
     6781 gtgttcaaca gtatctttcc tttggatgta tccatagctt tggttaaagc aagcttttca
     6841 tcagaagaag accatggttt tgagacttca acattaccgt tatgtttagc aagtgaaact
     6901 aaaggccctt catcctttct ggcagactta gaagacccct tattaccaaa aaaagtcaac
     6961 attttgagtt caacagcctg aggtagagga gcttgtgtaa tgtaattgat tgattcttag
     7021 caaagtgtat aataggtata tttctgatgc actgattgct ctgggtcggc atggcatctc
     7081 cacctcctcg cggtccgacc tgggcatccg aaggaggacg tcgtccactc ggatggctaa
     7141 gggagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     7201 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     7261 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     7321 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     7381 tatcgcgcgc ggtgtcatct atgttactag atcggaattc agattgtcgt ttcccgcctt
     7441 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     7501 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
     7561 gtatgtgcat gccaaccaca ggagatctca gtaaagcgaa aagtgccacc tgaacgaagc
     7621 atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
     7681 gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg
     7741 aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa
     7801 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac
     7861 caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt
     7921 tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct
     7981 tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt
     8041 ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct
     8101 gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg
     8161 cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat
     8221 gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta
     8281 ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata
     8341 ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag
     8401 gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga gatacttttg
     8461 agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt
     8521 ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt
     8581 tcctatactt tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac
     8641 gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc
     8701 tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta
     8761 tgcttaaatg cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc
     8821 ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag
     8881 ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc
     8941 ctttgatatt ggatcatact aagaaaccat tattatcatg acattaacct ataaaaatag
     9001 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     9061 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     9121 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     9181 agagcagatt gtactgagag tgcaccatag atcaacgaca ttactatata tataatatag
     9241 gaagcattta atagaacagc atcgtaatat atgtgtactt tgcagttatg acgccagatg
     9301 gcagtagtgg aagatattct ttattgaaaa atagcttgtc accttacgta caatcttgat
     9361 ccggagcttt tctttttttg ccgattaaga attaattcgg tcgaaaaaag aaaaggagag
     9421 ggccaagagg gagggcattg gtgactattg agcacgtgag tatacgtgat taagcacaca
     9481 aaggcagctt ggagtatgtc tgttattaat ttcacaggta gttctggtcc attggtgaaa
     9541 gtttgcggct tgcagagcac agaggccgca gaatgtgctc tagattccga tgctgacttg
     9601 ctgggtatta tatgtgtgcc caatagaaag agaacaattg acccggttat tgcaaggaaa
     9661 atttcaagtc ttgtaaaagc atataaaaat agttcaggca ctccgaaata cttggttggc
     9721 gtgtttcgta atcaacctaa ggaggatgtt ttggctctgg tcaatgatta cggcattgat
     9781 atcgtccaac tgcatggaga tgagtcgtgg caagaatacc aagagttcct cggtttgcca
     9841 gttattaaaa gactcgtatt tccaaaagac tgcaacatac tactcagtgc agcttcacag
     9901 aaacctcatt cgtttattcc cttgtttgat tcagaagcag gtgggacagg tgaacttttg
     9961 gattggaact cgatttctga ctgggttgga aggcaagaga gccccgaaag cttacatttt
    10021 atgttagctg gtggactgac gccagaaaat gttggtgatg cgcttagatt aaatggcgtt
    10081 attggtgttg atgtaagcgg aggtgtggag acaaatggtg taaaagactc taacaaaata
    10141 gcaaatttcg tcaaaaatgc taagaaatag gttattactg agtagtattt atttaagtat
    10201 tgtttgtgca cttgccgatc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    10261 taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
    10321 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
    10381 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
    10441 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
    10501 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
    10561 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
    10621 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
    10681 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt
    10741 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagat
    10801 ccttgctggc tgaaccccca gccggaactg accccacaag gccctagcgt ttgcaatgca
    10861 ccaggtcatc attgacccag gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg
    10921 cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga atccgatccg cacatgaggc
    10981 ggaaggtttc cagcttgagc gggtacggct cccggtgcga gctgaaatag tcgaacatcc
    11041 gtcgggccgt cggcgacagc ttgcggtact tctcccatat gaatttcgtg tagtggtcgc
    11101 cagcaaacag cacgacgatt tcctcgtcga tcaggacctg gcaacgggac gttttcttgc
    11161 cacggtccag gacgcggaag cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt
    11221 cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt
    11281 aataccggcc attgatcgac cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga
    11341 tcggctcgcc gataggggtg cgcttcgcgt actccaacac ctgctgccac accagttcgt
    11401 catcgtcggc ccgcagctcg acgccggtgt aggtgatctt cacgtccttg ttgacgtgga
    11461 aaatgacctt gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg
    11521 cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga
    11581 acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct
    11641 tggcctcgct gacctgtttt gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca
    11701 tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc tgttcgagac
    11761 gacgcgaacg ctccacggcg gccgatggcg cgggcagggc agggggagcc agttgcacgc
    11821 tgtcgcgctc gatcttggcc gtagcttgct gggccatcga gccgacggac tggaaggttt
    11881 cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc
    11941 ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc attcaccctc
    12001 cttgcgggat tgccccgact cacgccgggg caatgtgccc ttattcctga tttgacccgc
    12061 ctggtgcctt ggtgtccaga taatccacct tatcggcaat gaagtcggtc ccgtagaccg
    12121 tctggccgtc cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat accagcgacc
    12181 ccttgcccaa atacttgccg tgggcctcgg cctgagagcc aaaacacttg atgcggaaga
    12241 agtcggtgcg ctcctgcttg tcgccggcat cgttgcgcca catctaggta ctaaaacaat
    12301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
    12361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
    12421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
    12481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
    12541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggggtg
    12601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
    12661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
    12721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
    12781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
    12841 tgccgttcaa ggtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
    12901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
    12961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
    13021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
    13081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
    13141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaactttaaa taccagaaaa
    13201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
    13261 tgaaaccaca attatgggtg atgctgcaac tcgagcgggc cgggagggtt cgagaagggg
    13321 gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg ttaaaaacaa
    13381 ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg ccgaaaaacg
    13441 ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca aatgtcaata
    13501 ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc gcccctcatc
    13561 tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc aggcttgtcc
    13621 acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc gaggctggcc
    13681 agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc gcgccgggtg
    13741 agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc aagttttccg
    13801 cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg
    13861 gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc agcccggtga
    13921 gcgtctctag tggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
    13981 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
    14041 tagacaactt aataacacat tgcggacgtt tttaatgtac tggggtggtt tt
//
