LOCUS       sequence-390035-       10153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  35S promoter-driven expression of the positive-sense TSWV S RNA 
            segment encoding crRNA:GFP fusion in place of the NSs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Liu Q, Zhao C, Sun K, Deng Y, Li Z
  TITLE     Engineered biocontainable RNA virus vectors for non-transgenic 
            genome editing across crop species and genotypes.
  JOURNAL   Mol Plant. 2023 Mar 6;16(3):616-631. doi: 
            10.1016/j.molp.2023.02.003. Epub 2023 Feb 7.
  PUBMED    36751129
REFERENCE   2  (bases 1 to 10153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molp.2023.02.003"; journalName: "Mol Plant"; date: 
            "2023-03-6- 6"; volume: "16"; issue: "3"; pages: "616-631"
COMMENT     Sequence Label: pS-cr:GFP
FEATURES             Location/Qualifiers
     source          1..10153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        423..768
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     711..731
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             complement(join(2241..2954,2955..2957,2958..2960))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVPLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2286..2307)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2635..2654
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2894..2915
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      3222..3474
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3522..3546
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      3674..5016
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(4637..4684)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     5057..5075
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5113..5135)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5235..5254
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5275..5556
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             5557..6231
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(6334..6789)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6471..6492)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6683..6702
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(6905..8053)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8352..9146)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9355..9986
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    10066..10090
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
       61 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
      121 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
      181 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
      241 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag
      361 cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca
      421 attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct
      481 atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat
      541 tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga
      601 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
      661 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
      721 caagaccctt cctctatata aggaagttca tttcatttgg agaggagagc aattgtgtca
      781 attttattca aaccttaaca ctcagtctta caaatcatca cattaaaacc ctaagaaacg
      841 actgcggaat acagagttgt actttcgcac cttgaattac atacggtcaa agcatataac
      901 aacttctacg atcatcatgt ctaaggttaa gctcactaag gaaaacattg ttgctttgtt
      961 gacacaaggc aaagatcttg aatttgaaga agatcagaat ctggtagcat ttaacttcaa
     1021 gactttttgt ctggaaaacc ttgaccagat caagaagatg agcattattt catgtctgac
     1081 attcctgaag aatcgtcaga gtataatgaa ggttattaag caaagtgatt ttacttttgg
     1141 caaaatcact ataaagaaaa cttcagacag gattggagcc actgacatga ccttcagaag
     1201 gcttgatagc ttgatcaggg tcaggcttgt cgaggaaact gggaattctg agaatctcaa
     1261 tactatcaaa tctaagattg cttctcaccc tctgattcaa gcctatggat tacctcttga
     1321 tgatgcaaag tctgtgaggc ttgccataat gctgggaggt agcttacctc ttattgcttc
     1381 agttgatagc tttgagatga tcagtgttgt cttggctata tatcaggatg caaaatacaa
     1441 agacctcggg atcgatccaa agaagtatga caccagggaa gccttaggaa aagtttgcac
     1501 tgtgctaaaa agcaaagcat ttgaaatgaa tgaagatcag gtgaagaaag ggaaagagta
     1561 tgctgctata cttagctcca gcaatcctaa tgctaaagga agtattgcta tggaacatta
     1621 cagtgaaact cttaacaagt tctatgaaat gttcggggtt aaaaaacaga caaaacttgc
     1681 agaacttgct taaaagcagt tgtaagttaa attataaaaa agcctataaa tatataaagc
     1741 tttctttatc tttattgctt gtgcttgctt agtgtgttaa atttaaaata agtgtgttta
     1801 attaaagttt gctttctgtg tgttgtgctt aataataaac aaaataacaa aaacaacaaa
     1861 aaacataaaa taaaaataag aaaaaataaa ataaaataaa ataaaataaa taaataaaaa
     1921 taaaaataaa ataaaaacaa aaaacaaaaa acaaaaaaca aaacaaaacc caaatttggc
     1981 caaaattgtc cctttcggga tctttttggg tttttgtttt ttaatttttt gttgttttta
     2041 tttcattttt tgatttttgt ttgattttgt ttcatttttt gatttttgtt tcattttgat
     2101 tttattttta tttttatttt tatttttatg gtttctatta aacaggagaa attcaaaaga
     2161 aatgacaaaa cagagaaata attatgagta aagaaagaaa ataaacataa cataattagg
     2221 aaaagctgga cacggcaaga ttacttgtac agctcgtcca tgccgagagt gatcccggcg
     2281 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
     2341 gcggactggg tgctcaggta gtggttgtcg ggcagcggca cggggccgtc gccgatgggg
     2401 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
     2461 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
     2521 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
     2581 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
     2641 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
     2701 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg
     2761 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
     2821 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
     2881 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
     2941 tcctcgccct tgctcaccat actagtatct acacttagta gaaattggag accgaggtcc
     3001 gaggtctcca tctacactta gtagaaatta ctagttatgg ttattggtac tgtgttctta
     3061 ttacagtatt gtgattttct aagtgaggtt tgattaagaa taaaattatg acacaattgc
     3121 tctgggtcgg catggcatct ccacctcctc gcggtccgac ctgggcatcc gaaggaggac
     3181 gtcgtccact cggatggcta agggagagct cgaatttccc cgatcgttca aacatttggc
     3241 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
     3301 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     3361 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     3421 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcggaatt
     3481 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg
     3541 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     3601 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac aggagatctc agtaaagcga
     3661 aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag
     3721 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     3781 gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac
     3841 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
     3901 aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat
     3961 gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt
     4021 gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag
     4081 aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt
     4141 ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat
     4201 attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt
     4261 cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata
     4321 ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat
     4381 ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga
     4441 tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata
     4501 tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta
     4561 gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg
     4621 ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa
     4681 cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca
     4741 gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa
     4801 gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga
     4861 tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct
     4921 tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc
     4981 atccttcaat gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat
     5041 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     5101 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     5161 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     5221 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata gatcaacgac
     5281 attactatat atataatata ggaagcattt aatagaacag catcgtaata tatgtgtact
     5341 ttgcagttat gacgccagat ggcagtagtg gaagatattc tttattgaaa aatagcttgt
     5401 caccttacgt acaatcttga tccggagctt ttcttttttt gccgattaag aattaattcg
     5461 gtcgaaaaaa gaaaaggaga gggccaagag ggagggcatt ggtgactatt gagcacgtga
     5521 gtatacgtga ttaagcacac aaaggcagct tggagtatgt ctgttattaa tttcacaggt
     5581 agttctggtc cattggtgaa agtttgcggc ttgcagagca cagaggccgc agaatgtgct
     5641 ctagattccg atgctgactt gctgggtatt atatgtgtgc ccaatagaaa gagaacaatt
     5701 gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag catataaaaa tagttcaggc
     5761 actccgaaat acttggttgg cgtgtttcgt aatcaaccta aggaggatgt tttggctctg
     5821 gtcaatgatt acggcattga tatcgtccaa ctgcatggag atgagtcgtg gcaagaatac
     5881 caagagttcc tcggtttgcc agttattaaa agactcgtat ttccaaaaga ctgcaacata
     5941 ctactcagtg cagcttcaca gaaacctcat tcgtttattc ccttgtttga ttcagaagca
     6001 ggtgggacag gtgaactttt ggattggaac tcgatttctg actgggttgg aaggcaagag
     6061 agccccgaaa gcttacattt tatgttagct ggtggactga cgccagaaaa tgttggtgat
     6121 gcgcttagat taaatggcgt tattggtgtt gatgtaagcg gaggtgtgga gacaaatggt
     6181 gtaaaagact ctaacaaaat agcaaatttc gtcaaaaatg ctaagaaata ggttattact
     6241 gagtagtatt tatttaagta ttgtttgtgc acttgccgat ctatgcggtg tgaaataccg
     6301 cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa
     6361 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     6421 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     6481 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     6541 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     6601 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     6661 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     6721 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     6781 agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     6841 ctcttcgcta ttacgccaga tccttgctgg ctgaaccccc agccggaact gaccccacaa
     6901 ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc
     6961 tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg
     7021 aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg
     7081 agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata
     7141 tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct
     7201 ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg
     7261 attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca
     7321 ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa
     7381 gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca
     7441 cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct
     7501 tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct
     7561 tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt
     7621 ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt
     7681 gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg
     7741 tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac
     7801 ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg
     7861 cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tgggccatcg
     7921 agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt
     7981 cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa
     8041 acgtccgatt cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc
     8101 cttattcctg atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa
     8161 tgaagtcggt cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc
     8221 cctgcacgaa taccagcgac cccttgccca aatacttgcc gtgggcctcg gcctgagagc
     8281 caaaacactt gatgcggaag aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc
     8341 acatctaggt actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca
     8401 ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc
     8461 ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca
     8521 agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg
     8581 ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg
     8641 cgcggatctt taaatggggt gtcttcttcc cagttttcgc aatccacatc ggccagatcg
     8701 ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa
     8761 tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt
     8821 tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg
     8881 agcagattgc tccagccatc atgccgttca aggtgcagga cctttggaac aggcagcttt
     8941 ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac
     9001 cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta
     9061 gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat
     9121 tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa
     9181 agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct
     9241 aaaactttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca
     9301 tagtatcgac ggagccgatt ttgaaaccac aattatgggt gatgctgcaa ctcgagcggg
     9361 ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg
     9421 cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag
     9481 gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg
     9541 acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg
     9601 tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg
     9661 cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt
     9721 cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat
     9781 ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt
     9841 cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac
     9901 acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg
     9961 cgagggcaac cagcccggtg agcgtctcta gtggactgat gggctgcctg tatcgagtgg
    10021 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
    10081 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta
    10141 ctggggtggt ttt
//
