LOCUS       sequence-390039-        3031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses LuxR under the control of BBa_J23102 constitutive 
            promoter, ColE1 origin of replication, Ampicillin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Sarkar K, Chakraborty S, Bonnerjee D, Bagh S
  TITLE     Distributed Computing with Engineered Bacteria and Its Application 
            in Solving Chemically Generated 2 x 2 Maze Problems.
  JOURNAL   ACS Synth Biol. 2021 Oct 15;10(10):2456-2464. doi: 
            10.1021/acssynbio.1c00279. Epub 2021 Sep 20.
  PUBMED    34543017
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00279"; journalName: "ACS Synth Biol"; date: 
            "2021-10-15- 15"; volume: "10"; issue: "10"; pages: "2456-2464"
COMMENT     Sequence Label: pR
FEATURES             Location/Qualifiers
     source          1..3031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(82..168)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             985..996
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(1006..1040)
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     rep_origin      complement(1236..1824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1316..1335)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1912..2006)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2036..2827,2828..2896))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2659..2678
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2897..3001)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctactgggac gaagacgaac agtcgacaac gaagacgcca acatcaagat ctcgcatttg
       61 ttcctaggtc tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
      121 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctctagat
      181 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaat ttttaaagta
      241 tgggcaatca attgctcctg ttaaaattgc tttagaaata ctttggcagc ggtttgttgt
      301 attgagtttc atttgcgcat tggttaaatg gaaagtgaca gtacgctcac tgcaacctaa
      361 tatttttgaa atatcccaag agctttttcc ttcgcatgcc cacgctaaac attctttttc
      421 tcttttggtt aaatcgttgt ttgatttatt atttgctata tttatttttc gataattatc
      481 aactagagaa ggaacaatta atggtatgtt catacacgca tgtaaaaata aactatctat
      541 atagttgtct ttttctgaat gtgcaaaact aagcattccg aagccattgt tagccgtatg
      601 aatagggaaa ctaaacccag tgataagacc tgatgttttc gcttctttaa ttacatttgg
      661 agatttttta tttacagcat tgttttcaaa tatattccaa ttaattggtg aatgattgga
      721 gttagaataa tctactatag gatcatattt tattaaatta gcgtcatcat aatattgcct
      781 ccatttttta gggtaattat ctaggattga aatatcagat ttaaccatag aatgaggata
      841 aatgatcgcg agtaaataat attcacaatg taccatttta gtcatatcag ataagcattg
      901 attaatatca ttattgcttc tacaagcttt aattttatta attattctgt atgtgtcgtc
      961 ggcatttatg tttttcatgg tacctttctc ctctttaatg aattcgctag cacagtacct
     1021 aggactgagc tagctgtcaa ctcgagttac caggatcctg gtagaactgc agttccgtgc
     1081 tccaaaacaa ccgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1141 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1201 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1261 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1321 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1381 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1441 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1501 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1561 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1621 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     1681 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1741 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1801 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1861 cagtggaacg aaaactcacg ttaagggatt ttggtcatga ctagtgcttg gattctcacc
     1921 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     1981 cattactgga tctatcaaca ggagtccaag cgagctcgta aacttggtct gacagttacc
     2041 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2101 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2161 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2221 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2281 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2341 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2401 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2461 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2521 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2581 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2641 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2701 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2761 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2821 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2881 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2941 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3001 gcacatttcc ccgaaaagtg ccacctgacg t
//
