LOCUS       sequence-390040-       15968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of ALPK2 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15968)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 15968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-ALPK2-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..15968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4168..4188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4188..5399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5236..5255
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5400..5424
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(8691..8702)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(11947..11971)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11973..12014
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             12021..12791
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(12797..12817)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    12846..13434
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12899..12919)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13317..13328)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        13448..13947
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(13483..13506)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     13880..13899
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             13968..14567
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(13968..13987)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     14464..14484
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             14705..14938
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    15010..15131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15047..15066)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15101..15120
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      15171..15306
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(15326..15345)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(15327..15345)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(15355..15372)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(15355..15371)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15364..15386)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      15513..15941
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15600..15619)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15810..15831
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggy ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagaca
     5401 caagtttgta caaaaaagca ggcttagcca ccatgaaaga ctccgaaggg ccccagaggc
     5461 ccccgctgtg ttttttatct acattgcttt cccagaaggt tcctgagaag tcagacgctg
     5521 tgcttcgctg cataatatct ggtcagccca agccagaggt aacttggtat aagaatggtc
     5581 aggccatcga tgggagtggc attatttcca actatgaatt ctttgagaat cagtatattc
     5641 atgtgttaca tctctcttgc tgtaccaaaa atgatgctgc tgtctatcaa atctcggcta
     5701 aaaactcttt tggaatgatc tgttgttctg cttccgttga ggttgagtgc tcatcagaga
     5761 acccacaatt gtctcctaac ctggaagatg acagggacag gggttggaaa catgaaacag
     5821 ggacacatga agaagaaagg gcaaatcaga ttgatgagaa ggaacatcct tataaggaag
     5881 aagaaagcat ctccccgggc actcccaggt cagctgactc ctccccctcc aaatccaacc
     5941 attcactctc cctccagtca ttgggcaatc ttgacattag tgtgtccagt tctgaaaatc
     6001 ctttgggtgt taaaggaaca aggcacactg gagaggctta tgatccaagt aacacagaag
     6061 aaattgcaaa tgggttgctt tttcttaatt caagtcatat ttatgaaaaa caagacagat
     6121 gttgccacaa gacagtgcat tccatggcat caaagttcac ggatggtgac ctgaacaatg
     6181 atggtcctca tgatgaaggc ttacgctcta gtcagcaaaa tcccaaagta cagaaataca
     6241 ttagcttcag cctcccgcta tctgaggcaa ctgcacacat ttacccaggt gacagtgccg
     6301 tggccaacaa acaacccagc ccacagcttt ccagtgaaga ctctgacagt gactatgaac
     6361 tttgcccaga gataacccta acctacaccg aggagttttc agatgatgac ctggagtatc
     6421 tggaatgttc tgatgttatg acggattact ctaatgcagt ttggcaaagg aacctgctgg
     6481 ggactgagca tgttttttta ttagaaagcg atgacgaaga gatggaattc ggtgagcatt
     6541 gcctgggtgg gtgtgagcat ttcctcagtg gaatgggttg tgggtctcgg gtgtcgggtg
     6601 acgctgggcc tatggttgcc actgctggct tctgtggtca tcactcacaa ccccaagaag
     6661 ttggggtgag gagcagcaga gtctccaagc acggtccctc atccccacaa acagggatga
     6721 ctctcatttt gggacctcac caggatggaa cgtcttcagt gacagaacag gggagatata
     6781 aactccccac tgctcccgag gctgctgaaa atgattatcc aggaattcaa ggagaaacca
     6841 gagacagcca ccaagcaaga gaggaatttg ccagtgacaa tctgctcaac atggatgaat
     6901 cagtaagaga gacagagatg aagctcttgt ctggtgagtc agaaaactca gggatgagcc
     6961 agtgttggga gacggcagct gacaagagag tggggggaaa ggacttatgg agcaagaggg
     7021 gttcaaggaa atctgccagg gtgaggcagc cgggaatgaa gggaaatccc aagaagccga
     7081 atgccaacct gagagaaagt acaacagaag gtacccttca tctctgctct gccaaagaat
     7141 ctgctgagcc cccactaacc cagagtgata aaagagagac ttctcacacc acagcagcag
     7201 cgactggtcg gagttcccat gctgatgcaa gagaatgtgc tatttcaacc caggcagagc
     7261 aagaagcaaa aacccttcaa acttcaacag actcagtctc caaagaaggc aacacaaatt
     7321 gcaagggaga aggcatgcaa gttaatactc tatttgaaac aagccaggtt ccagactgga
     7381 gtgatcctcc tcaggtacaa gttcaggaaa cagtcagaga gacaatctct tgcagccaga
     7441 tgccagcttt ctcagagcct gctggggagg agtccccatt cactgggacc acaacaattt
     7501 ccttctcaaa cttaggaggg gtccacaagg aaaatgcatc attagctcaa cactcggagg
     7561 tcaaaccctg tacctgtggt ccacagcatg aagaaaaaca agacagagat ggcaacatac
     7621 ctgacaattt cagggaagac ctaaaatatg agcagagcat ctcagaagcc aatgatgaga
     7681 ctatgtcccc aggtgtgttc tcaaggcatc tccccaagga tgctcgtgct gacttcaggg
     7741 agcctgtggc tgtctctgtt gcttcccctg aacccacaga tactgccctc accctggaaa
     7801 atgtgtgtga tgagccaagg gacagagaag cagtgtgtgc aatggagtgt tttgaggctg
     7861 gtgaccaagg aacgtgtttt gataccatag attctcttgt tgggagacca gttgataaat
     7921 attcgcctca agaaatttgc tctgtagata cggaactggc agaaggtcaa aacaaagtat
     7981 ctgatttatg ttcttctaat gacaagacac tggaagtctt ttttcagaca caagtgtctg
     8041 agacttcagt gtctacgtgc aaaagcagca aggacggcaa ctcagtcatg tcccctcttt
     8101 ttaccagtac tttcaccttg aacatttcac acacagctag tgaaggtgcc acaggagaaa
     8161 atctagccaa ggtggagaat tccacctacc cactggcctc cacagtacat gctggccagg
     8221 agcagccaag ccccagcaac tcaggagggc ttgatgaaac acagctcctt tcttctgaga
     8281 acaatccttt agtgcaattt aaagaaggag gtgacaagag ccccagtcct agtgccgcag
     8341 acaccacagc cacaccagcc agttatagtt caattgtgag ttttccttgg gagaagccaa
     8401 caacattaac tgctaataat gagtgctttc aagcgaccag ggagactgag gacacatcaa
     8461 ctgttaccat tgccaccgaa gtccacccag ccaaatacct tgctgtgtca attcctgagg
     8521 acaagcatgc aggtggcact gaggagaggt tccctcgtgc atcccatgaa aaggtttccc
     8581 aatttccttc ccaagtgcag ttggatcata ttttaagtgg tgctaccatc aaatctacaa
     8641 aagagctact ttgcagggca cccagtgtgc caggagtccc acaccatgtc ctgcagctcc
     8701 cagagggaga gggtttctgc agtaattccc ctcttcaggt tgataacctg tctggagata
     8761 agagccagac tgtggacaga gcagacttta ggagctatga agagaatttc caagaaagag
     8821 gaagtgaaac aaagcagggg gtccagcagc agagcctgtc ccagcagggt tctctttctg
     8881 cacctgattt ccaacaaagt ttgcctacga catctgctgc acaagaggaa agaaacttgg
     8941 tgcccacggc ccactcaccc gcaagctcta gggaaggagc agggcagcgc tcaggttggg
     9001 ggacgagggt ctccgtggtg gctgaaactg ctggggaaga agacagtcag gctctgagca
     9061 acgttccatc tctctctgat atccttttgg aagagtctaa agaatataga cctggaaatt
     9121 gggaggcagg caacaagctg aagattataa ctctagaggc ttccgcttct gaaatctggc
     9181 caccacgaca actgacaaat tctgagagca aggcatcaga cggtggtctc ataattcctg
     9241 acaaggtctg ggctgtacct gatagtctaa aggcagatgc tgttgtgcct gaattggccc
     9301 cctctgaaat agcagcattg gctcacagtc cagaggatgc tgagtcagcc cttgctgata
     9361 gcagagaaag ccataaaggc gaagagccca ccatcagtgt acattggaga agtctttctt
     9421 cccggggttt cagccaaccc agactcctgg agtcatccgt ggaccctgta gatgaaaagg
     9481 agttatctgt cacagattca ctgtcagcgg cttctgaaac tggagggaag gaaaatgtta
     9541 acaatgtgag tcaagaccag gaggaaaaac aactcaagat ggatcacact gccttcttta
     9601 aaaagtttct gacctgccct aaaatcctag agtcctctgt agatcccatt gatgagataa
     9661 gtgtgataga gtacaccagg gctggaaaac cagagccctc tgaaaccaca ccacagggcg
     9721 ccagagaagg gggtcaatca aatgacggaa acatgggcca cgaagcggaa atccagccgg
     9781 ccattttgca agttccatgt ctccagggaa ccattctgag tgaaaataga atcagcagaa
     9841 gccaagaagg cagtatgaag caggaggctg aacaaattca acctgaggag gcaaaaactg
     9901 ccatttggca agtcctgcaa cccagcgaag gcggtgaaag aattccaagt ggatgtagca
     9961 taggccaaat acaagaaagc agtgatggga gcttagggga ggctgagcaa agcaaaaagg
    10021 acaaagcaga attgatttcc cccacttcac ctctttctag ttgtcttcca ataatgactc
    10081 acgcttctct tggggttgac acgcacaact ccacaggcca aattcatgac gtccctgaaa
    10141 atgacatagt tgagcccaga aagcgtcagt atgtgtttcc tgtttcacag aaaaggggaa
    10201 ctattgagaa tgagcgtggg aaacctttgc cctcttctcc tgatcttacc aggttccctt
    10261 gtacttcatc tcctgaagga aatgtcacag actttttgat aagccacaaa atggaggaac
    10321 ctaaaataga ggtgcttcaa attggggaaa ccaaaccccc aagctcatct agctcctcag
    10381 cgaagacctt ggcatttatt tcaggagaac gtgagttaga gaaagcccct aaattactgc
    10441 aggatccatg tcaaaagggc accctgggct gtgcgaaaaa gtccagggag agagagaagt
    10501 ccctggaagc ccgagcaggc aaatcgccag ggaccctcac agcagtgacg gggtcagagg
    10561 aggtcaagag gaagccagaa gccccaggca gtggacattt agctgaggga gtaaagaaga
    10621 aaattttgtc cagggtggca gcactgaggc tgaaactgga agaaaaggaa aatatcagaa
    10681 agaactcagc ctttcttaaa aagatgccca aactcgaaac atcattatca cacacagaag
    10741 agaaacaaga cccaaaaaag ccatcttgca aaagagaagg aagagctcca gtattactga
    10801 aaaagatcca agctgagatg ttccctgaac actctggaaa tgtaaaatta agctgccaat
    10861 ttgcagaaat tcatgaagat tctactatct gctggacaaa agattcaaag tccatagccc
    10921 aagtgcagag aagtgcaggg gacaactcca ctgtttcctt tgccatcgtg caagccagtc
    10981 cgaaggacca gggactctat tactgctgca tcaagaacag ctacggaaaa gtgactgctg
    11041 aatttaacct cacagctgaa gttctcaaac agctgtcaag tcgccaggat actaaaggat
    11101 gtgaagagat tgaattcagc caactcatct tcaaagaaga cttcctccat gacagctact
    11161 ttgggggccg cctgcgtggt cagatcgcca cggaggagct gcactttgga gaaggggttc
    11221 accgcaaagc cttccgcagc acagtgatgc acggcctcat gcctgtcttc aaacctggcc
    11281 atgcctgtgt gcttaaggtg cacaatgcca ttgcctatgg gaccagaaat aatgatgagc
    11341 tcatccaaag gaactacaaa ctcgctgccc aggaatgcta tgttcaaaat actgccaggt
    11401 attatgccaa gatctacgct gctgaagcac agcctctgga aggctttgga gaagtacctg
    11461 agatcattcc tatttttctt atccatcggc ctgagaacaa tatcccgtat gctacagtgg
    11521 aggaggagct gattggagaa tttgtgaagt attccatcag ggatgggaaa gaaataaact
    11581 tcttgagaag agaatcagaa gctggtcaga aatgttgcac cttccagcac tgggtgtacc
    11641 agaaaacaag tggctgcctc ctggtgacgg acatgcaagg tgtaggaatg aagctaactg
    11701 acgttggcat agcaacgctg gctaaagggt acaagggatt taaaggcaac tgttccatga
    11761 ccttcattga tcagtttaaa gcactacacc agtgtaacaa gtattgcaaa atgctgggac
    11821 tgaaatccct tcaaaacaac aaccagaaac agaagcagcc gagcattggg aaaagcaaag
    11881 ttcaaacaaa ctctatgaca ataaagaagg cagggcctga gaccccaggc gaaaagaaaa
    11941 cctcaaaccc agctttcttg tacaaagtgg gcggcaagcc catccccaac cccctgctgg
    12001 gcctggacag caccgctagc atcccgctgc tgaacgctaa acagattctg ggacagctgg
    12061 acggcgggag cgtggcagtc ctgcctgtgg tcgactccac caatcagtac ctgctggatc
    12121 gaatcggcga gctgaagagt ggggatgctt gcattgcaga atatcagcag gcagggagag
    12181 gaagcagagg gaggaaatgg ttctctcctt ttggagctaa cctgtacctg agtatgtttt
    12241 ggcgcctgaa gcggggacca gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg
    12301 cagaagcgct gcgaaagctg ggagcagaca aggtgcgagt caaatggccc aatgacctgt
    12361 atctgcagga tagaaagctg gcaggcatcc tggtggagct ggccggaata acaggcgatg
    12421 ctgcacagat cgtcattggc gccgggatta acgtggctat gaggcgcgtg gaggaaagcg
    12481 tggtcaatca gggctggatc acactgcagg aagcagggat taacctggac aggaatactc
    12541 tggccgctat gctgatccga gagctgcggg cagccctgga actgttcgag caggaaggcc
    12601 tggctccata tctgtcacgg tgggagaagc tggataactt catcaataga cccgtgaagc
    12661 tgatcattgg ggacaaagag attttcggga ttagccgggg gattgataaa cagggagccc
    12721 tgctgctgga acaggacgga gttatcaaac cctggatggg cggagaaatc agtctgcggt
    12781 ctgccgaaaa gtaagccaac tttattatac atagttgata tccagcacag tggcggccgc
    12841 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
    12901 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
    12961 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
    13021 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
    13081 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
    13141 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
    13201 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
    13261 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
    13321 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
    13381 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
    13441 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
    13501 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
    13561 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
    13621 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
    13681 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
    13741 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
    13801 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
    13861 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
    13921 tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca
    13981 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
    14041 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
    14101 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
    14161 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
    14221 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
    14281 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
    14341 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
    14401 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
    14461 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
    14521 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
    14581 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atctcgaggg cccggtacct
    14641 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
    14701 ggactggaag ggctagctca ctcccaacga agacaagatc tgctttttgc ttgtactggg
    14761 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    14821 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    14881 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
    14941 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
    15001 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    15061 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    15121 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
    15181 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    15241 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
    15301 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
    15361 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
    15421 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
    15481 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
    15541 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
    15601 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
    15661 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    15721 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    15781 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    15841 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    15901 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
    15961 tacaattt
//
