LOCUS       sequence-390049-       12673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express eGFP fluorescence after Y93H correction and mCherry as 
            transfection control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Rieffer AE, Chen Y, Salamango DJ, Moraes SN, Harris RS
  TITLE     APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels.
  JOURNAL   CRISPR J. 2023 Oct;6(5):430-446. doi: 10.1089/crispr.2023.0027. Epub
            2023 Sep 6.
  PUBMED    37672599
REFERENCE   2  (bases 1 to 12673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2023.0027"; journalName: "CRISPR J"; date: 
            "2023-10"; volume: "6"; issue: "5"; pages: "430-446"
COMMENT     Sequence Label: eGFP Y93H CC editing reporter
FEATURES             Location/Qualifiers
     source          1..12673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             481..738
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1903..2136
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2321..2365
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2659..2685
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     enhancer        3002..3305
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3306..3509
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3460..3480
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3506..3530
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3546..4253
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3691..3709)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3959..3978
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4278..4331
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(4377..4398)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4638..4657)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4985..5006
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5079..5647
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5241..5258)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5468..5487
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5686..6705
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5686..6705
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_RNA        6865..6940
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     LTR             7028..7661
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9301..9318)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9472..10060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9552..9571)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10231..11022,11023..11091))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10854..10873
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11092..11196)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11264..11282
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11320..11342)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11442..11461
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atcagagtcc ttggtgtgga gggagggacc agcgcagctt ccagccatcc acctgatgaa
       61 cagaacctag ggaaagcccc agttctactt acaccaggaa aggctggaag ggctaatttg
      121 gtcccaaaaa agacaagaga tccttgatct gtggatctac cacacacaag gctacttccc
      181 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
      241 cttcaagtta gtaccagttg aaccagagca agtagaagag gccaatgaag gagagaacaa
      301 cagcttgtta caccctatga gccagcatgg gatggaggac ccggagggag aagtattagt
      361 gtggaagttt gacagcctcc tagcatttcg tcacatggcc cgagagctgc atccggagta
      421 ctacaaagac tgctgacatc gagctttcta caagggactt tccgctgggg actttccagg
      481 gaggtgtggc ctgggcggga ctggggagtg gcgagccctc agatgctaca tataagcagc
      541 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      601 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg ctcaaagtag
      661 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      721 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag taaagccaga
      781 ggagatctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      841 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      901 cgagagcgtc ggtattaagc gggggagaat tagataaatg ggaaaaaatt cggttaaggc
      961 cagggggaaa gaaacaatat aaactaaaac atatagtatg ggcaagcagg gagctagaac
     1021 gattcgcagt taatcctggc cttttagaga catcagaagg ctgtagacaa atactgggac
     1081 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacaatag
     1141 cagtcctcta ttgtgtgcat caaaggatag atgtaaaaga caccaaggaa gccttagata
     1201 agatagagga agagcaaaac aaaagtaaga aaaaggcaca gcaagcagca gctgacacag
     1261 gaaacaacag ccaggtcagc caaaattacc ctatagtgca gaacctccag gggcaaatgg
     1321 tacatcaggc catatcacct agaactttaa atcgacatag cagaataggc gttactcgac
     1381 agaggagagc aagaaatgga gccagtagat cctagactag agccctggaa gcatccagga
     1441 agtcagccta aaactgcttg taccaattgc tattgtaaaa agtgttgctt tcattgccaa
     1501 gtttgtttca tgacaaaagc cttaggcatc tcctatggca ggaagaagcg gagacagcga
     1561 cgaagagctc atcagaacag tcagactcat caagcttctc tatcaaagca gtaagtagta
     1621 catgggcgcg cccatgtggc aggaagtagg aaaagcaatg tatgcccctc ccatcagtgg
     1681 acaaattaga tgttcatcaa atattactgg gctgctatta acaagagatg gtggtaataa
     1741 caacaatggg tccgagatct tcagacctgg aggaggcgat atgagggaca attggagaag
     1801 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1861 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1921 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1981 cagacaatta ttgtctgata tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     2041 gcaacagcat ctgttgcaac tcacagtctg gggcatcaaa cagctccagg caagaatcct
     2101 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2161 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2221 gatttggaat aacatgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2281 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2341 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2401 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2461 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2521 cccaatcccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2581 cagagacaga tccattcgat tagtgaacgg atccttagca cttatctggg acgatctgcg
     2641 gagcctgtgc ctcttcagct accaccgctt gagagactta ctcttgattg taacgaggat
     2701 tgtggaactt ctgggacgca gggggtggga agccctcaaa tattggtgga atctcctaca
     2761 atattggagt caggagctaa agaatagtgc tgttagcttg ctcaatgcca cagccatagc
     2821 agtagctgag gggacagata gggttataga agtagtacaa ggagcttcta gagctattcg
     2881 ccacatacct agaagaataa gacagggctt ggaaaggatt ttgctataag atgggtggcg
     2941 cggccgctta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     3001 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     3061 tgacgtcaat aatgacgtat gttcccatag taacgccaat tgggactttc cattgacgtc
     3121 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     3181 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     3241 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     3301 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     3361 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     3421 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     3481 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc cagctggcta
     3541 ccggtatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     3601 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     3661 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     3721 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     3781 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     3841 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     3901 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     3961 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     4021 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     4081 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     4141 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     4201 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagcccggtg
     4261 cagatctcga gtacaaggag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
     4321 atcccggccc tatggtcagt aaaggtgaag aactgttcac cggggtggtg cccatcctgg
     4381 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     4441 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     4501 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     4561 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggccac gtccaggagc
     4621 ggaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     4681 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     4741 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     4801 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     4861 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4921 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     4981 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggt atggatgaat
     5041 tatataaata acgtacgctt aattaacgga tccggaattc cgctcccccc cccctaacgt
     5101 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     5161 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     5221 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     5281 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     5341 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     5401 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     5461 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     5521 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     5581 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     5641 atgataagct tgccacaacc cgtaccaaag atggatagat ccggaaagcc tgaactcacc
     5701 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     5761 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     5821 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     5881 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
     5941 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     6001 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     6061 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     6121 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     6181 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     6241 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     6301 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     6361 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     6421 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     6481 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     6541 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     6601 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     6661 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagagtag atgccgaccg
     6721 aacaaggcat gctcttcaac ctcaataacg ttatatcctg attcacgtaa tatttttggg
     6781 gtaaatttag ttcctgttcc attaactgcg ctaaaaataa tttttaaatc ttttttagct
     6841 tcttgctctt ttttgtacgt ctctgtttta gagctagaaa tagcaagtta aaataaggct
     6901 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgaat tcgttcggta
     6961 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7021 gggggactgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     7081 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc aggggtcaga
     7141 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     7201 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tggaatggat
     7261 gaccctgaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     7321 gcccgagagc tgcatccgga gtacttcaag aactgctgac atcgagcttg ctacaaggga
     7381 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     7441 ctcagatgct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     7501 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7561 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7621 atccctcaga cccttttagt cagtgtggaa aatctctagc acccaggagg tagaggttgc
     7681 agtgagccaa gatcgcgcca ctgcattcca gcctgggcaa gaaaacaaga ctgtctaaaa
     7741 taataataat aagttaaggg tattaaatat atttatacat ggaggtcata aaaatatata
     7801 tatttgggct gggcgcagtg gctcacacct gcgcccggcc ctttgggagg ccgaggcagg
     7861 tggatcacct gagtttggga gttccagacc agcctgacca acatggagaa accccttctc
     7921 tgtgtatttt tagtagattt tattttatgt gtattttatt cacaggtatt tctggaaaac
     7981 tgaaactgtt tttcctctac tctgatacca caagaatcat cagcacagag gaagacttct
     8041 gtgatcaaat gtggtgggag agggaggttt tcaccagcac atgagcagtc agttctgccg
     8101 cagactcggc gggtgtcctt cggttcagtt ccaacaccgc ctgcctggag agaggtcaga
     8161 ccacagggtg agggctcagt ccccaagaca taaacaccca agacataaac acccaacagg
     8221 tccaccccgc ctgctgccca ggcagagccg attcaccaag acgggaatta ggatagagaa
     8281 agagtaagtc acacagagcc ggctgtgcgg gagaacggag ttctattatg actcaaatca
     8341 gtctccccaa gcattcgggg atcagagttt ttaaggataa cttagtgtgt agggggccag
     8401 tgagttggag atgaaagcgt agggagtcga aggtgtcctt ttgcgccgag tcagttcctg
     8461 ggtgggggcc acaagatcgg atgagccagt ttatcaatcc gggggtgcca gctgatccat
     8521 ggagtgcagg gtctgcaaaa tatctcaagc actgattgat cttaggtttt acaatagtga
     8581 tgttacccca ggaacaattt ggggaaggtc agaatcttgt agcctgtagc tgcatgactc
     8641 ctaaaccata atttcttttt tgtttttttt tttttatttt tgagacaggg tctcactctg
     8701 tcacctaggc tggagtgcag tggtgcaatc acagctcact gcagcctcaa cgtcgtaagc
     8761 tcaagcgatc ctcccacctc agcctgcctg gtagctgaga ctacaagcga cgccccagtt
     8821 aatttttgta tttttggtag aggcagcgtt ttgccgtgtg gccctggctg gtctcgaact
     8881 cctgggctca agtgatccag cctcagcctc ccaaagtgct gggacaaccg gggccagtca
     8941 ctgcacctgg ccctaaacca taatttctaa tcttttggct aatttgttag tcctacaaag
     9001 gcagtctagt ccccaggcaa aaagggggtt tgtttcggga aagggctgtt actgtctttg
     9061 tttcaaacta taaactaagt tcctcctaaa cttagttcgg cctacaccca ggaatgaaca
     9121 aggagagctt ggaggttaga agcacgatgg aattggttag gtcagatctc tttcactgtc
     9181 tgagttataa ttttgcaatg gtggttcaaa gactgcccgc ttctgacacc agtcgctgca
     9241 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     9301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     9361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     9421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9901 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    10021 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    10081 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    10141 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    10201 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    10261 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    10321 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    10381 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    10441 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    10501 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    10561 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    10621 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    10681 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    10741 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10801 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10861 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10921 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10981 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    11041 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    11101 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    11161 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    11221 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    11281 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    11341 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    11401 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
    11461 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
    11521 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    11581 ctcttcgcta ttacgccagg ggaggcagag attgcagtaa gctgagatcg cagcactgca
    11641 ctccagcctg ggcgacagag taagactctg tctcaaaaat aaaataaata aatcaatcag
    11701 atattccaat cttttccttt atttatttat ttattttcta ttttggaaac acagtccttc
    11761 cttattccag aattacacat atattctatt tttctttata tgctccagtt ttttttagac
    11821 cttcacctga aatgtgtgta tacaaaatct aggccagtcc agcagagcct aaaggtaaaa
    11881 aataaaataa taaaaaataa ataaaatcta gctcactcct tcacatcaaa atggagatac
    11941 agctgttagc attaaatacc aaataaccca tcttgtcctc aataatttta agcgcctctc
    12001 tccaccacat ctaactcctg tcaaaggcat gtgccccttc cgggcgctct gctgtgctgc
    12061 caaccaactg gcatgtggac tctgcagggt ccctaactgc caagccccac agtgtgccct
    12121 gaggctgccc cttccttcta gcggctgccc ccactcggct ttgctttccc tagtttcagt
    12181 tacttgcgtt cagccaaggt ctgaaactag gtgcgcacag agcggtaaga ctgcgagaga
    12241 aagagaccag ctttacaggg ggtttatcac agtgcaccct gacagtcgtc agcctcacag
    12301 ggggtttatc acattgcacc ctgacagtcg tcagcctcac agggggttta tcacagtgca
    12361 cccttacaat cattccattt gattcacaat ttttttagtc tctactgtgc ctaacttgta
    12421 agttaaattt gatcagaggt gtgttcccag aggggaaaac agtatataca gggttcagta
    12481 ctatcgcatt tcaggcctcc acctgggtct tggaatgtgt cccccgaggg gtgatgacta
    12541 cctcagttgg atctccacag gtcacagtga cacaagataa ccaagacacc tcccaaggct
    12601 accacaatgg gccgccctcc acgtgcacat ggccggagga actgccatgt cggaggtgca
    12661 agcacacctg cgc
//
