LOCUS       sequence-39005-e        3734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   He A, Kong SW, Ma Q, Pu WT
  TITLE     Co-occupancy by multiple cardiac transcription factors identifies 
            transcriptional enhancers active in heart.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7.
  PUBMED    21415370
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Apr 5. 108(14):5632-7."
COMMENT     Sequence Label: pEntr-GATA4flbio
FEATURES             Location/Qualifiers
     source          1..3734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     25..48
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    67..166
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1558..1581
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1594..1638
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(1656..1672)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1656..1672)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(1672..1771)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1883..1902)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1894..2703
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1961..1980)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2793..3381
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3282..3301
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      3546..3632
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      join(3724..3734,1..17)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctgacggatg gcctttttgc gtttctacaa actcttcctg ttagttagtt acttaagctc
       61 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
      121 aagcaatgct tttttataat gccaactttg tacaaaaaag caggctcttt aaaggaacca
      181 attcagtcga ctggatccgg taccttcgaa ctcgagacca tggagtcgac gtaccaaagc
      241 ctggccatgg ccgccaacca cggccccccg cccggcgcct acgaagcagg tggccctggc
      301 gccttcatgc acagcgcggg cgccgcgtcc tcgcccgtct acgtgcccac tccgcgggtg
      361 ccgtcctctg tgctgggcct gtcctacctg cagggcggtg gcagtgccgc tgcagctgga
      421 accacctcgg gtggcagctc cggggccggc ccgtcgggtg cagggcctgg gacccagcag
      481 ggtagccctg gctggagcca agctggagcc gagggagccg cctacacccc gccgcccgtg
      541 tccccgcgct tctctttccc ggggactact gggtccctgg cggccgctgc cgccgctgcc
      601 gcagcccggg aagctgcagc ctacggcagt ggcggcgggg cggcgggcgc tggtctggct
      661 ggccgagagc agtacgggcg tccgggcttc gccggctcct actccagccc ctacccagcc
      721 tacatggccg acgtgggagc atcctgggcc gcagccgctg ccgcctctgc cggccccttc
      781 gacagcccag tcctgcacag cctgcctgga cgggccaacc ctggaagaca ccccaatctc
      841 gatatgtttg atgacttctc agaaggcaga gagtgtgtca attgtggggc catgtccacc
      901 ccactctgga ggcgagatgg gacgggacac tacctgtgca atgcctgtgg cctctatcac
      961 aagatgaacg gcatcaaccg gcccctcatt aagcctcagc gccgcctgtc cgcttcccgc
     1021 cgggtaggcc tctcctgtgc caactgccag actaccacca ccacgctgtg gcgtcgtaat
     1081 gccgagggtg agcctgtatg taatgcctgc ggcctctaca tgaagctcca tggggttccc
     1141 aggcctcttg caatgcggaa ggaggggatt caaaccagaa aacggaagcc caagaacctg
     1201 aataaatcta agacgccagc aggtcctgct ggtgagaccc tccctccctc cagtggtgcc
     1261 tccagcggta actccagcaa tgccactagc agcagcagca gcagtgaaga gatgcgcccc
     1321 atcaagacag agcccgggct gtcatctcac tatgggcaca gcagctccat gtcccagaca
     1381 ttcagtactg tgtccggcca cgggccctcc atccatccag tgctgtctgc tctgaagctg
     1441 tccccacaag gctatgcatc tcctgtcact cagacatcgc aggccagctc caagcaggac
     1501 tcttggaaca gcctggtcct ggctgacagt catggggaca taatcaccgc gagatctgac
     1561 tacaaagacg atgacgataa aggtgatggg tccggcctga acgacatctt cgaggctcag
     1621 aaaatcgaat ggcacgaagg cgcgccgagc tcgaggatcc actagttcta gacccagctt
     1681 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
     1741 tcactatcag tcaaaataaa atcattattt gccatccagc tgcagctctg gcccgtgtct
     1801 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     1861 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     1921 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     1981 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     2041 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     2101 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     2161 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     2221 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     2281 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     2341 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     2401 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     2461 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     2521 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2581 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     2641 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     2701 taatcagaat tggttaattg gttgtaacat tattcagatt gggccccgtt ccactgagcg
     2761 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2821 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2881 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2941 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3001 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3061 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3121 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3181 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3241 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3301 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3361 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3421 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3481 attaccgcta gcatggatct cggggacgtc taactactaa gcgagagtag ggaactgcca
     3541 ggcatcaaat aaaacgaaag gctcagtcgg aagactgggc ctttcgtttt atctgttgtt
     3601 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgtga
     3661 agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaacta
     3721 agcagaaggc catc
//
