LOCUS       sequence-390051-       12673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express eGFP fluorescence after Y93H correction and mCherry as 
            transfection control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Rieffer AE, Chen Y, Salamango DJ, Moraes SN, Harris RS
  TITLE     APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels.
  JOURNAL   CRISPR J. 2023 Oct;6(5):430-446. doi: 10.1089/crispr.2023.0027. Epub
            2023 Sep 6.
  PUBMED    37672599
REFERENCE   2  (bases 1 to 12673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2023.0027"; journalName: "CRISPR J"; date: 
            "2023-10"; volume: "6"; issue: "5"; pages: "430-446"
COMMENT     Sequence Label: eGFP Y93H GC editing reporter
FEATURES             Location/Qualifiers
     source          1..12673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             489..746
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1911..2144
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2329..2373
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2667..2693
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     enhancer        3010..3313
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3314..3517
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3468..3488
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3514..3538
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3554..4261
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3699..3717)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3967..3986
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4286..4339
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(4385..4406)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4646..4665)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4993..5014
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5087..5655
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5249..5266)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5476..5495
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5694..6713
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5694..6713
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_RNA        6873..6948
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     LTR             7036..7669
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9309..9326)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9480..10068)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9560..9579)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10239..11030,11031..11099))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10862..10881
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11100..11204)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11272..11290
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11328..11350)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11450..11469
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acctgcgcat cagagtcctt ggtgtggagg gagggaccag cgcagcttcc agccatccac
       61 ctgatgaaca gaacctaggg aaagccccag ttctacttac accaggaaag gctggaaggg
      121 ctaatttggt cccaaaaaag acaagagatc cttgatctgt ggatctacca cacacaaggc
      181 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
      241 ggatggtgct tcaagttagt accagttgaa ccagagcaag tagaagaggc caatgaagga
      301 gagaacaaca gcttgttaca ccctatgagc cagcatggga tggaggaccc ggagggagaa
      361 gtattagtgt ggaagtttga cagcctccta gcatttcgtc acatggcccg agagctgcat
      421 ccggagtact acaaagactg ctgacatcga gctttctaca agggactttc cgctggggac
      481 tttccaggga ggtgtggcct gggcgggact ggggagtggc gagccctcag atgctacata
      541 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
      601 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      661 caaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
      721 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaagta
      781 aagccagagg agatctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
      841 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
      901 gatgggtgcg agagcgtcgg tattaagcgg gggagaatta gataaatggg aaaaaattcg
      961 gttaaggcca gggggaaaga aacaatataa actaaaacat atagtatggg caagcaggga
     1021 gctagaacga ttcgcagtta atcctggcct tttagagaca tcagaaggct gtagacaaat
     1081 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1141 tacaatagca gtcctctatt gtgtgcatca aaggatagat gtaaaagaca ccaaggaagc
     1201 cttagataag atagaggaag agcaaaacaa aagtaagaaa aaggcacagc aagcagcagc
     1261 tgacacagga aacaacagcc aggtcagcca aaattaccct atagtgcaga acctccaggg
     1321 gcaaatggta catcaggcca tatcacctag aactttaaat cgacatagca gaataggcgt
     1381 tactcgacag aggagagcaa gaaatggagc cagtagatcc tagactagag ccctggaagc
     1441 atccaggaag tcagcctaaa actgcttgta ccaattgcta ttgtaaaaag tgttgctttc
     1501 attgccaagt ttgtttcatg acaaaagcct taggcatctc ctatggcagg aagaagcgga
     1561 gacagcgacg aagagctcat cagaacagtc agactcatca agcttctcta tcaaagcagt
     1621 aagtagtaca tgggcgcgcc catgtggcag gaagtaggaa aagcaatgta tgcccctccc
     1681 atcagtggac aaattagatg ttcatcaaat attactgggc tgctattaac aagagatggt
     1741 ggtaataaca acaatgggtc cgagatcttc agacctggag gaggcgatat gagggacaat
     1801 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     1861 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     1921 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1981 gtacaggcca gacaattatt gtctgatata gtgcagcagc agaacaattt gctgagggct
     2041 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaaaca gctccaggca
     2101 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2161 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2221 ctggaacaga tttggaataa catgacctgg atggagtggg acagagaaat taacaattac
     2281 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2341 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2401 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2461 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2521 acccacctcc caatcccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     2581 gagagagaca gagacagatc cattcgatta gtgaacggat ccttagcact tatctgggac
     2641 gatctgcgga gcctgtgcct cttcagctac caccgcttga gagacttact cttgattgta
     2701 acgaggattg tggaacttct gggacgcagg gggtgggaag ccctcaaata ttggtggaat
     2761 ctcctacaat attggagtca ggagctaaag aatagtgctg ttagcttgct caatgccaca
     2821 gccatagcag tagctgaggg gacagatagg gttatagaag tagtacaagg agcttctaga
     2881 gctattcgcc acatacctag aagaataaga cagggcttgg aaaggatttt gctataagat
     2941 gggtggcgcg gccgcttaat agtaatcaat tacggggtca ttagttcata gcccatatat
     3001 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     3061 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaattg ggactttcca
     3121 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     3181 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     3241 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     3301 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     3361 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     3421 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3481 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcca
     3541 gctggctacc ggtatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     3601 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     3661 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     3721 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     3781 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     3841 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     3901 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     3961 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     4021 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     4081 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     4141 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     4201 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     4261 gcccggtgca gatctcgagt acaaggaggg cagaggaagt cttctaacat gcggtgacgt
     4321 ggaggagaat cccggcccta tggtcagtaa aggtgaagaa ctgttcaccg gggtggtgcc
     4381 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4441 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4501 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     4561 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aagggcacgt
     4621 ccaggagcgg accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4681 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4741 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     4801 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     4861 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4921 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     4981 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggtat
     5041 ggatgaatta tataaataac gtacgcttaa ttaacggatc cggaattccg ctcccccccc
     5101 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     5161 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     5221 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     5281 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     5341 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     5401 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     5461 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     5521 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     5581 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     5641 aaaacacgat gataagcttg ccacaacccg taccaaagat ggatagatcc ggaaagcctg
     5701 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
     5761 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     5821 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     5881 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
     5941 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
     6001 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
     6061 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     6121 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     6181 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     6241 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     6301 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     6361 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     6421 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     6481 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     6541 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     6601 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
     6661 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagagtagat
     6721 gccgaccgaa caaggcatgc tcttcaacct caataacgtt atatcctgat tcacgtaata
     6781 tttttggggt aaatttagtt cctgttccat taactgcgct aaaaataatt tttaaatctt
     6841 ttttagcttc ttgctctttt ttgtacgtct ctgttttaga gctagaaata gcaagttaaa
     6901 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgaattc
     6961 gttcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7021 aagaaaaggg gggactggaa gggctaattc actcccaaag aagacaagat atccttgatc
     7081 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     7141 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     7201 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     7261 gaatggatga ccctgagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     7321 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgacat cgagcttgct
     7381 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     7441 ggcgagccct cagatgctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     7501 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7561 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7621 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcac ccaggaggta
     7681 gaggttgcag tgagccaaga tcgcgccact gcattccagc ctgggcaaga aaacaagact
     7741 gtctaaaata ataataataa gttaagggta ttaaatatat ttatacatgg aggtcataaa
     7801 aatatatata tttgggctgg gcgcagtggc tcacacctgc gcccggccct ttgggaggcc
     7861 gaggcaggtg gatcacctga gtttgggagt tccagaccag cctgaccaac atggagaaac
     7921 cccttctctg tgtattttta gtagatttta ttttatgtgt attttattca caggtatttc
     7981 tggaaaactg aaactgtttt tcctctactc tgataccaca agaatcatca gcacagagga
     8041 agacttctgt gatcaaatgt ggtgggagag ggaggttttc accagcacat gagcagtcag
     8101 ttctgccgca gactcggcgg gtgtccttcg gttcagttcc aacaccgcct gcctggagag
     8161 aggtcagacc acagggtgag ggctcagtcc ccaagacata aacacccaag acataaacac
     8221 ccaacaggtc caccccgcct gctgcccagg cagagccgat tcaccaagac gggaattagg
     8281 atagagaaag agtaagtcac acagagccgg ctgtgcggga gaacggagtt ctattatgac
     8341 tcaaatcagt ctccccaagc attcggggat cagagttttt aaggataact tagtgtgtag
     8401 ggggccagtg agttggagat gaaagcgtag ggagtcgaag gtgtcctttt gcgccgagtc
     8461 agttcctggg tgggggccac aagatcggat gagccagttt atcaatccgg gggtgccagc
     8521 tgatccatgg agtgcagggt ctgcaaaata tctcaagcac tgattgatct taggttttac
     8581 aatagtgatg ttaccccagg aacaatttgg ggaaggtcag aatcttgtag cctgtagctg
     8641 catgactcct aaaccataat ttcttttttg tttttttttt tttatttttg agacagggtc
     8701 tcactctgtc acctaggctg gagtgcagtg gtgcaatcac agctcactgc agcctcaacg
     8761 tcgtaagctc aagcgatcct cccacctcag cctgcctggt agctgagact acaagcgacg
     8821 ccccagttaa tttttgtatt tttggtagag gcagcgtttt gccgtgtggc cctggctggt
     8881 ctcgaactcc tgggctcaag tgatccagcc tcagcctccc aaagtgctgg gacaaccggg
     8941 gccagtcact gcacctggcc ctaaaccata atttctaatc ttttggctaa tttgttagtc
     9001 ctacaaaggc agtctagtcc ccaggcaaaa agggggtttg tttcgggaaa gggctgttac
     9061 tgtctttgtt tcaaactata aactaagttc ctcctaaact tagttcggcc tacacccagg
     9121 aatgaacaag gagagcttgg aggttagaag cacgatggaa ttggttaggt cagatctctt
     9181 tcactgtctg agttataatt ttgcaatggt ggttcaaaga ctgcccgctt ctgacaccag
     9241 tcgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9301 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9361 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     9421 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9481 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9541 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9601 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9661 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9721 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9781 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9841 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9901 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9961 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    10021 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10081 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    10141 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    10201 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10261 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10321 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10381 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10441 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    10501 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    10561 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    10621 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10681 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10741 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10801 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10861 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10921 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10981 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    11041 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    11101 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    11161 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    11221 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    11281 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    11341 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    11401 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
    11461 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    11521 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
    11581 gtgcgggcct cttcgctatt acgccagggg aggcagagat tgcagtaagc tgagatcgca
    11641 gcactgcact ccagcctggg cgacagagta agactctgtc tcaaaaataa aataaataaa
    11701 tcaatcagat attccaatct tttcctttat ttatttattt attttctatt ttggaaacac
    11761 agtccttcct tattccagaa ttacacatat attctatttt tctttatatg ctccagtttt
    11821 ttttagacct tcacctgaaa tgtgtgtata caaaatctag gccagtccag cagagcctaa
    11881 aggtaaaaaa taaaataata aaaaataaat aaaatctagc tcactccttc acatcaaaat
    11941 ggagatacag ctgttagcat taaataccaa ataacccatc ttgtcctcaa taattttaag
    12001 cgcctctctc caccacatct aactcctgtc aaaggcatgt gccccttccg ggcgctctgc
    12061 tgtgctgcca accaactggc atgtggactc tgcagggtcc ctaactgcca agccccacag
    12121 tgtgccctga ggctgcccct tccttctagc ggctgccccc actcggcttt gctttcccta
    12181 gtttcagtta cttgcgttca gccaaggtct gaaactaggt gcgcacagag cggtaagact
    12241 gcgagagaaa gagaccagct ttacaggggg tttatcacag tgcaccctga cagtcgtcag
    12301 cctcacaggg ggtttatcac attgcaccct gacagtcgtc agcctcacag ggggtttatc
    12361 acagtgcacc cttacaatca ttccatttga ttcacaattt ttttagtctc tactgtgcct
    12421 aacttgtaag ttaaatttga tcagaggtgt gttcccagag gggaaaacag tatatacagg
    12481 gttcagtact atcgcatttc aggcctccac ctgggtcttg gaatgtgtcc cccgaggggt
    12541 gatgactacc tcagttggat ctccacaggt cacagtgaca caagataacc aagacacctc
    12601 ccaaggctac cacaatgggc cgccctccac gtgcacatgg ccggaggaac tgccatgtcg
    12661 gaggtgcaag cac
//
