LOCUS       sequence-39006-e        3362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   He A, Kong SW, Ma Q, Pu WT
  TITLE     Co-occupancy by multiple cardiac transcription factors identifies 
            transcriptional enhancers active in heart.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7.
  PUBMED    21415370
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Apr 5. 108(14):5632-7."
COMMENT     Sequence Label: pEntr-Nkx2-5flbio
FEATURES             Location/Qualifiers
     source          1..3362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1466..1489
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1502..1546
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     protein_bind    complement(1591..1690)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1802..1821)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1813..2622
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSVRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1880..1899)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2712..3300
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3201..3220
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg
      481 acgccgccgc gatcgcatgt tccccagccc tgcgctcaca cccacgcctt tctcagtcaa
      541 agacatcctg aacctggagc agcagcagcg tagcctggcg tctggggacc tgtctgcgcg
      601 cctcgaggcc accctggccc ctgcctcctg catgctggcc gccttcaagc ccgaggccta
      661 ctctggcccc gaggcggcag cgtccggcct ggcagagctg cgcgcggaga tgggccccgc
      721 gccttcgccc cccaagtgct ctcctgcttt cccagccgcc cccacatttt acccgggagc
      781 ctacggtgac cctgacccag ccaaagaccc tcgggcggat aaaaaagagc tgtgcgcgct
      841 gcagaaggca gtggagctgg acaaagccga gacggatggc gccgagagac cacgcgcacg
      901 gcggcgacgg aagccacgcg tgctcttctc gcaggcgcag gtctacgagc tggagcggcg
      961 cttcaagcaa cagcggtacc tgtcggcgcc agagcgcgac cagctggcca gcgtgctgaa
     1021 gctcacgtcc acgcaggtca agatctggtt ccagaaccgt cgctacaagt gcaagcgaca
     1081 gcggcaggac cagactctgg agcttctggg gccgccgccg ccgcccgcgc gcaggatcgc
     1141 ggtgcccgtg ctggtgcgcg acgggaagcc ctgcctgggg gaccccgcgg cctacgctcc
     1201 cgcctacggc gtgggtctca atgcctatgg ctacaacgcc tacccctacc ccagctacgg
     1261 cggcgcggcc tgtagtcccg gctacagctg cgccgcctac cccgctgcgc cccccgccgc
     1321 gcagcccccc gccgcctccg ccaacagcaa cttcgtgaac tttggcgtcg gggacttgaa
     1381 caccgtgcag agtcccggga tgccgcaggg caattcgggc gtctccacgc tgcacggcat
     1441 ccgagcctgg agcggaccga cgcgtgacta caaagacgat gacgataaag gtgatgggtc
     1501 cggcctgaac gacatcttcg aggctcagaa aatcgaatgg cacgaaggcg cgccgagctc
     1561 gaggtaggcg gccgcactcg agatatctag acccagcttt cttgtacaaa gttggcatta
     1621 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     1681 tcattatttg ccatccagct gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     1741 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     1801 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     1861 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1921 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1981 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     2041 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     2101 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     2161 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     2221 tgtcctttta acagcgttcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     2281 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     2341 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     2401 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     2461 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     2521 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     2581 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     2641 ttgtaacatt attcagattg ggccccgttc cactgagcgt cagaccccgt agaaaagatc
     2701 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2761 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2821 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2881 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2941 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3001 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3061 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3121 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3181 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3241 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3301 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3361 tt
//
