LOCUS       sequence-390060-        1257 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway middle entry vector expressing constitutively active 
            zebrafish map2k2a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Greysson-Wong J, Rode R, Ryu JR, Chan JL, Davari P, Rinker KD, 
            Childs SJ
  TITLE     rasa1-related arteriovenous malformation is driven by aberrant 
            venous signalling.
  JOURNAL   Development. 2023 Sep 15;150(18):dev201820. doi: 10.1242/dev.201820.
            Epub 2023 Sep 14.
  PUBMED    37708300
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2023-09-15"; volume: "150(18):dev201820. 
            doi"; pages: " 10.1242/dev.201820. Epub 2023 Sep 14"
COMMENT     Sequence Label: pME map2k2a_s219D
FEATURES             Location/Qualifiers
     source          1..1257
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..29
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1229..1253)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggacaagt ttgtacaaaa aagcaggctc caccatggca ccaaaaagaa gaccagtccc
       61 cttgattata acccccactg gagagggaca gtcaaccaac attgatgcag cagcagaggc
      121 caacctggag gccttgcaga ggaagttagg tgagctggat ttggatgaac agcagaggaa
      181 gcgcctcgaa gctttcctca cccagaaagc ccaggtgggc gagctcaagg atgaagattt
      241 cgaacccata tgtgaacttg gcgctggtaa tggtggagtg gttcataaag tccgtcacaa
      301 accctcgaga ctggttatgg ccaggaagct tatacacttg gaaatcaaac cagccatcag
      361 gaaccagatt attcgagagc tgcaggttct acacgagtgt aactcaccgt acattgtagg
      421 gttttacggc gccttctaca gtgatggaga aatcagcatc tgtatggagc acatggatgg
      481 aggctctttg gatcaagtgc tcaaagaagc cagaagaatc cctgaggaaa ttttgggcaa
      541 agttagcata gctgtactca gaggtttggc gtatcttcgg gagaagcacc aaataatgca
      601 cagagacgtt aagccctcca acatcctggt gaactcgcgc ggtgagatca aactgtgcga
      661 ctttggtgtg agtggccagc tcatcgactc tatggccaac tccttcgttg gaacacggtc
      721 gtacatgtcg ccggagagac tccagggcac gcactactcg gttcagtcgg atgtgtggag
      781 catgggccta tcactggttg agcttgctat tggacgctac cccatccccc ctcccgatgc
      841 taaggaactg gaggccatat ttggccggcc agtgctggat gcaggtgggg cagaaggtca
      901 cagcatgtct ccaagaccca ggcctccagg acgaccagtt agtggacacg gaatggacag
      961 caggccagca atggctatat ttgagctact ggactacatt gtcaatgagc ctccacccaa
     1021 gctcccccat ggcgtcttca ccacagactt tgaggagttt gtgacaaaat gcctcataaa
     1081 gaaccctgct gacagagctg acctcaagat gctaatgggt cacacgttca tcaagcgggc
     1141 tgaggttgag gaggtggatt ttgctggatg gttgtgtaaa accatgggcc ttcaccagcc
     1201 tagcacaccc actcatagcg ctgaatgaac ccagctttct tgtacaaagt ggtcccc
//
