LOCUS       sequence-390061-         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway middle entry vector expressing constitutively actve 
            zebrafish rras.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Greysson-Wong J, Rode R, Ryu JR, Chan JL, Davari P, Rinker KD, 
            Childs SJ
  TITLE     rasa1-related arteriovenous malformation is driven by aberrant 
            venous signalling.
  JOURNAL   Development. 2023 Sep 15;150(18):dev201820. doi: 10.1242/dev.201820.
            Epub 2023 Sep 14.
  PUBMED    37708300
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2023-09-15"; volume: "150(18):dev201820. 
            doi"; pages: " 10.1242/dev.201820. Epub 2023 Sep 14"
COMMENT     Sequence Label: pME rras_Q61R
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..29
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(629..653)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggacaagt ttgtacaaaa aagcaggctt caccatgtca acggaagaaa gatacaagtt
       61 ggtggttgtg ggcggtggag gtgtggggaa aagcgccctc accattcagt tcatccagtc
      121 gtactttgtg tctgactacg acccaaccat tgaagactct tacactaaaa tctgcaccgt
      181 ggacgggaag gagacacgat tagacatcct cgacacagca gggcgagagg agtttggagc
      241 catgagagag cagtacatgc gctctggaga gggcttctta ctggtgtttg ctcttaatga
      301 cagtggcagt tataacgaaa ttcagaaatt tcacactcag atattacggg ttaaggatcg
      361 agatgacttt cctatggttc ttgttggcaa caaatcagat cttgaccagc agagagtgat
      421 ctcaaaagag gaagccatga cgtttgcccg agaaaacagg atccactata tggaatcatc
      481 agccaagaac cgtcacaacg tggatgaagc cttcatggag gttgtgcgag caataaggaa
      541 gtttcaggag acagagagtc caccgcttcc cgctaatcat acagggaagc agaagagtgg
      601 tggctgtccc tgcaccctac tctgagtaac ccagctttct tgtacaaagt ggtcccc
//
