LOCUS       sequence-39008-e        3923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   He A, Kong SW, Ma Q, Pu WT
  TITLE     Co-occupancy by multiple cardiac transcription factors identifies 
            transcriptional enhancers active in heart.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7.
  PUBMED    21415370
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Apr 5. 108(14):5632-7."
COMMENT     Sequence Label: pEntr-SRFflbio
FEATURES             Location/Qualifiers
     source          1..3923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2027..2050
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2063..2107
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     protein_bind    complement(2152..2251)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2363..2382)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2374..3183
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSVRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2441..2460)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3273..3861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3762..3781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg
      481 acgccgccgc gatcgcatgt taccgaccca agctggggcc gcggcggctc tgggccgggg
      541 ctcggccctg gggggcagcc tgaaccggac cccgacgggg cggccgggcg gcggcggcgg
      601 gacacgcggg gctaacgggg gccgggtccc cgggaatggc gcggggctcg ggcccggccg
      661 cctggagcgg gaggctgcgg cagcggcggc aaccaccccg gcgcccaccg cgggggccct
      721 ctacagcggc agcgagggcg actcggagtc gggcgaggag gaggagctgg gcgccgagcg
      781 gcgcggcctg aagcggagcc tgagcgagat ggagatcggt atggtggtcg gtgggcccga
      841 ggcgtcggca gcggccaccg ggggctacgg gccggtgagc ggcgcggtga gcggggccaa
      901 gccgggtaag aagacccggg gccgcgtgaa gatcaagatg gagttcatcg acaacaagct
      961 gcggcgctac acgaccttca gcaagaggaa gacgggcatc atgaagaagg cctatgagct
     1021 gtccacgctg acagggacac aggtgctgtt gctggtggcc agtgagacag gccatgtgta
     1081 tacctttgcc acccgaaaac tgcagcccat gatcaccagt gagaccggca aggcactgat
     1141 tcagacctgc ctcaactcgc cagactctcc accccgttca gaccccacaa cagaccagag
     1201 aatgagtgcc actggctttg aagagacaga tctcacctac caggtgtcgg agtctgacag
     1261 cagtggggag accaaggaca cactgaagcc ggcgttcaca gtcaccaacc tgccgggtac
     1321 aacctccacc atccaaacag cacctagcac ctctaccacc atgcaagtca gcagcggccc
     1381 ctcctttccc atcaccaact acctggcacc agtgtctgct agtgtcagcc ccagtgctgt
     1441 cagcagtgcc aatgggactg tgctgaagag tacaggcagc ggccctgtct cctctggggg
     1501 ccttatgcag ctgcctacca gcttcaccct catgcctggt ggggcagtgg cccagcaggt
     1561 cccagtgcag gccattcaag tgcaccaggc cccacagcaa gcgtctccct cccgtgacag
     1621 cagcacagac ctcacgcaga cctcctccag cgggacagtg acgctgcccg ccaccatcat
     1681 gacgtcatcc gtgcccacaa ctgtgggtgg ccacatgatg taccctagcc cgcatgcggt
     1741 gatgtatgcc cccacctcgg gcctgggtga tggcagcctc accgtgctga atgccttctc
     1801 ccaggcacca tccaccatgc aggtgtcaca cagccaggtc caggagccag gtggcgtccc
     1861 ccaggtgttc ctgacagcat catctgggac agtgcagatc cctgtttcag cagttcagct
     1921 ccaccagatg gctgtgatag ggcagcaggc cgggagcagc agcaacctca ccgagctaca
     1981 ggtggtgaac ctggacaccg cccacagcac caagagtgaa acgcgtgact acaaagacga
     2041 tgacgataaa ggtgatgggt ccggcctgaa cgacatcttc gaggctcaga aaatcgaatg
     2101 gcacgaaggc gcgccgagct cgaggtaggc ggccgcactc gagatatcta gacccagctt
     2161 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
     2221 tcactatcag tcaaaataaa atcattattt gccatccagc tgcagctctg gcccgtgtct
     2281 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     2341 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     2401 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     2461 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     2521 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     2581 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     2641 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     2701 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     2761 cattcgattc ctgtttgtaa ttgtcctttt aacagcgttc gcgtatttcg tctcgctcag
     2821 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     2881 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     2941 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     3001 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     3061 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     3121 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     3181 taatcagaat tggttaattg gttgtaacat tattcagatt gggccccgtt ccactgagcg
     3241 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3301 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3361 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3421 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3481 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3541 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3601 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3661 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3721 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3781 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3841 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3901 tgctggcctt ttgctcacat gtt
//
