LOCUS       sequence-390102-        5561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the ASH neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pMZ43
FEATURES             Location/Qualifiers
     source          1..5561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2619..2768
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        3306..3410
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3411..3479,3480..4271)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3629..3648)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4442..5030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4931..4950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5184..5201
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5318..5339
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5354..5359,5360..5377,5378..5384)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5392..5408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5397..5419
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5416..5432
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5416..5432
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggcccaag aataaatgct tcgtttgtct
       61 ccctttctgt aaaatacgat gcaaaacgtt aaaaactcgc catttcattc ctgacattcc
      121 tgaaaaacaa gctaattgtt aagagagaaa atgggaaaaa ggaaaaatga gaagagaaag
      181 cgatttttta acgttttgta tcgtattcta cagaaagaga gacaaacaga gcatttttcg
      241 agtaaaaact gtgaaaactt aattatttct catttaaaaa atagttgaac acttttttat
      301 ccaaaaaaac gaacaaaatc gagttaaatc gtggaatttc gaacagaaaa cgaataaaaa
      361 actgcaaatt accgcacgaa acaagcgaga aaaaagcgtg ccgcaggctt gcgttgacgc
      421 tcaagacaaa actttttcaa ataatatatc ctataaggat tatcattatc attaatatta
      481 catcaactgg aatactggta tctttcaaga cacctagcat cattacagcg ggtctgggtg
      541 ctagaatcaa tggaatagtg aaaaccgagc aagtataatc agttgcgaca atccatccgt
      601 gaagaatcaa taggcatcgt ttaactgtga tcatttgcgt gggcgttttg aataaaatac
      661 aaaataatcc gaagacatga attggagctg atacaactga agcgatgtgg aaacttgata
      721 ttaaaaactc aggagtatca atgtatttta tatcaggggt acaagaaaaa tttatttttg
      781 acattggaat aaaatgagta gttgaaaagt ggaggtagtt acttgggcaa aaaatgaaaa
      841 aaaaaattga aaaaccagct gccctcggaa taatgaatat aatattaaaa atggaccaaa
      901 ttcagtgagg aattgtgttt tagaatcgaa catatttttt gcatttttat ttcctatttt
      961 ttaatgtttc catggaaaac attaaaaccc gcatggaatt caaatataaa acaacaacaa
     1021 aaaattgagt gagcaattag tgttgtatat ttacgtgcct gtaaaaaaaa aactgactga
     1081 tcaaatataa ttttaaacat cgaaacaaga atttgttact gaaaattgaa aaaagttttc
     1141 aaaaagtttt tttttctaat ttttttttaa accagctttt cattttgatt ctgataacat
     1201 acaaatcagg gcaaaccgga aaataaattt gatgaatcgc cgaaattttt gacaataatt
     1261 cataagaata tccaaattac gtgtaaaacc ctacagcgag agagagatcg tccaattttt
     1321 gaagttgtcg gaaaaatgtc atgaatatac gcaaatttac cgaaaactca tgctaaattt
     1381 gaaaattcga gttttgaata aaaattagaa agcattatat ataataatag aaaaaaaata
     1441 atttggaatg tttctgaaaa atattgtttt tttttcttga aaatttccca aagatacaca
     1501 tattcaaact gaatttttct tctgtttctg aaaattttga aatcatgcat ttccggcaaa
     1561 acccgcagtt atgctagttt tccctccgcc ggcaggagaa gagagacgca gacagcgaga
     1621 gtgtctcgtt tctctgcgct ctctcgaaat ttcaaagtta tcggaaattt gccatgatta
     1681 tacttatttt gactgggaaa atatgctgaa cacaaattta taagactaat agcttagaaa
     1741 aatgaagttt ttaaaatttc aaaaaaaaaa attccatttt atttttcaaa aacccgatta
     1801 ctttctatat tttgacgttt ctagaacaca gtttataatg taacaccaaa tcatttctca
     1861 gattaatcaa attcctcaga attcggaaca ttcggtcaga agtatacgtc gtagccaaac
     1921 tcaattaccc aaataatctg cacctacata aaatgctcat tttaccggaa aaagagcaca
     1981 taagctggcg cgcctctaga ggatccccgg gattggccaa aggacccaaa ggtatgtttc
     2041 gaatgatact aacataacat agaacatttt caggaggacc cttggctagg atgggcccta
     2101 aaaagaagcg taaagctagc atgaagctgt tgtcttcaat ggaacaagct tgcgacatat
     2161 gccggttaaa aaaactcagg tgctcgaagg aaaagcccaa gtgtgccaag tgtctgaaga
     2221 acaactcgga gtgccgttat tctcccaagg ctatgaggtc cccactgacc agggcgcatc
     2281 tgacagaggt agaatcaagg ttggaaaaat tggaagattt gtttctgctc atgtttcctc
     2341 gggaaaacct ggacagcatt ttgaacatgg actctttgga cgaagtgaaa gtaatgttga
     2401 aacaattata tatgcaggac tatggtgaca cggatgctgc tatggatagc ttgaccccta
     2461 tggaaaatgg catgtctgaa gggccgagaa aacacatgaa gactgcgaca tcatcgccaa
     2521 aagtggatga agatggaagc caaagtcagt tgagtgtatc gccggaattc ccgctgcagt
     2581 ctggtggcgg aggggctcct aggggttccg gacgggctga cgcattggac gattttgatc
     2641 tggatatgct gggaagtgac gccctcgatg attttgacct tgacatgctt ggttcggatg
     2701 cccttgatga ctttgacctc gacatgctcg gcagtgacgc ccttgatgat ttcgacctgg
     2761 acatgctgat taactaaggt accgaattcg ctagccggcc atacaagtaa tccggatgat
     2821 cgacgccaac gtcgttgaat tttcaaattt taaatactga atatttgttt tttttcctat
     2881 tatttattta ttctctttgt gttttttttc ttgctttcta aaaaattaat tcaatccaaa
     2941 tctaaacatt tttttttctc tttccgtctc ccaattcgta ttccgctcct ctcatctgaa
     3001 cacaatgtgc aagtttattt atcttctcgc tttcatttca ttaggacgtg gggggaattg
     3061 gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa taaggacaca caatatgcaa
     3121 caacattcaa ttcagaaata tggaggaagg tttaaaagaa aacataaaaa tatatagagg
     3181 aggaaggaaa actagtaaaa aataagcaaa gaaattaggc gaacgatgag aattgtcctc
     3241 acttgggccc aaaaggcctt ttttttttgg cgccgacgtc aggtggcact tttcggggaa
     3301 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3361 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3421 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3481 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3541 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3601 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3661 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3721 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3781 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3841 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3901 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3961 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4021 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4081 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4141 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4201 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4261 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4321 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4381 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4441 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4501 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4561 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4621 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4681 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4741 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4801 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4861 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4921 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4981 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5041 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5101 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5161 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5221 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     5281 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     5341 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     5401 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa
     5461 catgatctta ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat
     5521 cactcacaac gatggatacg ctaacaactt ggaaatgaaa t
//
