LOCUS       sequence-39012-e         601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 601)
  AUTHORS   White MD, Milne RV, Nolan MF
  TITLE     A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or 
            Down-Regulate Neuronal Gene Expression in vivo.
  JOURNAL   Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4.
  PUBMED    21772812
REFERENCE   2  (bases 1 to 601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2011"; volume: "4"; pages: "8. Epub 2011 Jul 4"
COMMENT     Sequence Label: pENTR-L3-HCN1miR-EGFP-L2
FEATURES             Location/Qualifiers
     source          1..601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..96
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           237..263
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           327..368
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     443..462
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      494..503
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(545..566)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 caataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat
       61 gcttttttat aatgccaact ttgtataata aagttgctaa ggtagccttg cagaagttgg
      121 tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag
      181 aaactgggct tgtcgagaca gagaagactc ttgcgtttcg gtcgacttct agagctctgg
      241 aggcttgctg aaggctgtat gctgtaaaga cgacagtagg tatcagtttt ggccactgac
      301 tgactgatac cttgtcgtct ttacaggaca caaggcctgt tactagcact cacatggaac
      361 aaatggccga attcggcgct agctgctgat atcgcatacg cgtggaccag ataggcacct
      421 attggtctta ctgacatcca ctttgccttt ctctccacag gtgtcggtac cgcgggcccg
      481 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      541 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      601 c
//
