LOCUS       sequence-39014-e         583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 583)
  AUTHORS   White MD, Milne RV, Nolan MF
  TITLE     A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or 
            Down-Regulate Neuronal Gene Expression in vivo.
  JOURNAL   Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4.
  PUBMED    21772812
REFERENCE   2  (bases 1 to 583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2011"; volume: "4"; pages: "8. Epub 2011 Jul 4"
COMMENT     Sequence Label: pENTR-R4r-HCN1miR-EGFP-R3r
FEATURES             Location/Qualifiers
     source          1..583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(28..151)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           292..318
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           382..423
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     498..517
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      549..558
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcactaatac catctaagta gttgattcat agtgactgga tatgttgtgt tttacagtat
       61 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
      121 gtttctcgtt caacttttct atacaaagtt gctaaggtag ccttgcagaa gttggtcgtg
      181 aggcactggg caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact
      241 gggcttgtcg agacagagaa gactcttgcg tttcggtcga cttctagagc tctggaggct
      301 tgctgaaggc tgtatgctgt aaagacgaca gtaggtatca gttttggcca ctgactgact
      361 gataccttgt cgtctttaca ggacacaagg cctgttacta gcactcacat ggaacaaatg
      421 gccgaattcg gcgctagctg ctgatatcgc atacgcgtgg accagatagg cacctattgg
      481 tcttactgac atccactttg cctttctctc cacaggtgtc ggtaccgcgg gcccgggatc
      541 caccggtcgc caccatggtg agcaagggcg aggagctgtt cac
//
